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Volume 13 Issue 5
Sep.  2022
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LUO Xin, PANG Kun, CHEN Jianxiong, WANG Hongzhe, XU Xinjie, LI Bing, JIA Xinmiao, YOU Xin. Differences of Intestinal Flora in Children with Autism Spectrum Disorder with Different Levels of Serum Total 25-hydroxyvitamin D[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(5): 812-820. DOI: 10.12290/xhyxzz.2022-0254
Citation: LUO Xin, PANG Kun, CHEN Jianxiong, WANG Hongzhe, XU Xinjie, LI Bing, JIA Xinmiao, YOU Xin. Differences of Intestinal Flora in Children with Autism Spectrum Disorder with Different Levels of Serum Total 25-hydroxyvitamin D[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(5): 812-820. DOI: 10.12290/xhyxzz.2022-0254
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Differences of Intestinal Flora in Children with Autism Spectrum Disorder with Different Levels of Serum Total 25-hydroxyvitamin D

  • 1.

    Department of Rheumatology and Clinical Immunology, Key Laboratory of Rheumatology & Clinical Immunology, Ministry of Education, National Clinical Research Center for Dermatologic and Immunologic Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China

  • 2.

    Institute of Genomic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China

  • 3.

    School of Information Technology & Management, University of International Business and Economics, Beijing 100029, China

  • 4.

    Medical Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China

Funds: 

CAMS Innovation Fund for Medical Sciences 2017-I2M-3-017

Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences 2019XK320030

More Information
  • Corresponding author:

    JIA Xinmiao, E-mail: jiaxinmiaohappy@126.com

    YOU Xin, E-mail: youxin@pumch.cn

  • Received Date: May 12, 2022
  • Accepted Date: June 26, 2022
  • Available Online: August 01, 2022
  • Issue Publish Date: September 29, 2022
    Graphical Abstract
    • Abstract
  •   Objective  To investigate the differences of intestinal flora in Vitamin D adequacy and deficiency groups of children with autism spectrum disorder(ASD) and the correlation between serum total 25-hydroxyvitamin D [T-25(OH)D] levels and intestinal flora.
      Methods  ASD children who attended the outpatient clinic of the department of Rheumatology and Clinical Immunology of Peking Union Medical College Hospital during October 2009 and February 2022 were retrospectively included in the study. According to the serum T-25(OH)D levels, they were divided into Vitamin D adequacy group[T-25(OH)D > 30 μg/L], Vitamin D insufficiency group[20 μg/L≤T-25(OH)D≤30 μg/L] and Vitamin D deficiency group[T-25(OH)D < 20 μg/L].Serum T-25(OH)D levels were measured using liquid chromatography-tandem mass spectrometry. Human gut metagenome data from these children with ASD were analyzed using bioinformatics methods.
      Results  46 children with ASD who met the inclusion and exclusion criteria were included in the study. The numbers of patients in Vitamin D adequacy group, Vitamin D insufficiency group and Vitamin D deficiency group were respectively 15, 16 and 15. Linear discriminant analysis revealed that the bacteria abundance of Bilophila wadsworthia, Adlercreutzia equolifaciens, Asaccharobacter celatus and Escherichia coli were significantly enriched, while the bacteria abundance of Bacteroides fragilis and Hungatella hathewayi were significantly lower in the Vitamin D deficiency group. The relative abundance of Bilophila wadsworthia and Adlercreutzia equolifaciens were negatively correlated with serum T-25(OH)D levels(r=-0.45, fdr=0.055, P=0.002;r=-0.44, fdr=0.055, P=0.003), and the relative abundance of Bacteroides fragilis was positively correlated with serum T-25(OH)D levels (r=0.42, fdr=0.073, P=0.004).
      Conclusions  Vitamin D deficiency in ASD may exacerbate ASD flora disorders, and decreased serum T-25(OH)D levels may facilitate potentially harmful bacteria but inhibit probiotic colonization. This study provides partial evidence that children with ASD should be actively supplemented with vitamin D.
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