Abstract: Cardiovascular disease (CVD) is one of the most serious diseases endangering human health at present, including myocardial ischemia syndrome, myocardial fibrosis, atrial fibrillation and other diseases. microRNAs (miRNAs) are a class of small non-coding RNA that regulate gene expression by recognizing homologous sequences and interfering with transcription, translation, or epigenetic processes. In recent years, it has been found that miR-223 is related to the occurrence and development of various CVD, and is a potential specific therapeutic target. This paper summarized the relevant studies of miR-223 in CVD by taking myocardial ischemia syndrome, myocardial fibrosis and atrial fibrillation as the starting point, and discussed its application prospects and challenges as a specific therapeutic target. It provides a new way of diagnosis and treatment of CVD.