Abstract: Objective To explore the value of portable oto-endoscopy system in clinical teaching of otolaryngology residents. Methods The postgraduate students serving as resident doctors in the Department of Otolaryngology of Peking Union Medical College Hospital from February to March 2022 and from February to March 2023 were selected as the research objects. Random number table method was used to divide them into experimental group and control group. The control group was first taught by theoretical explanation + electrootoscope system, and the experimental group was first taught by theoretical explanation + portable otoscope system. After one month, the two groups interchanged their teaching methodologies. The results of theoretical assessment, self-evaluation at the end of the first month of clinical learning and satisfaction with teaching effectiveness at the end of two months of clinical learning were compared between the two groups. Results A total of 36 residents were included in this study, with 18 in each group. After one month of clinical study, the theoretical test scores of the experimental group were significantly higher than those of the control group (93.17±4.16) points vs. (82.28±4.46) points, P=0.000. The results of the questionnaire survey showed that the self-evaluation scores of the test group in the five dimensions of ear disease learning, clinical learning efficiency, clinical data collection, doctor-patient communication and equipment use were significantly higher than those of the control group (all P <0.05). After two months of ear rotation, the satisfaction of the 36 students on portable ear endoscopy teaching was significantly higher than that of electric ear endoscopy (57.9% vs. 47.4%, P=0.040). Conclusion The portable oto-endoscope system can display the anatomy and diseases of otolaryngology more vividly and intuitively in the clinical teaching of otolaryngology, facilitate the management of clinical data, increase the learning interest of residents, fully mobilize the image thinking of medical students, and improve the post competence of residents more efficiently.