Abstract: Norovirus is a linear single-stranded positive-sense RNA virus. It is one of the main pathogens causing acute gastroenteritis in humans. The transmission route of norovirus infection is complex, along with a low infection dose. Currently there are different laboratory-based detection methods, including direct microscopy, immunological assay, and molecular biology test. Being on the alert for norovirus epidemics and outbreaks, regulating patient management, hand hygiene, implementation of cleaning and disinfection measures, and health education are the key points of prevention and control of norovirus infection.