Email Alert RSS
Volume 1 Issue 1
Jul.  2010
Turn off MathJax
Article Contents
Abstract
References
Related Articles
Wei-duan ZHONG, Li WANG, Jie CUI, Feng-chun ZHANG. Immune Response of Peripheral Blood Mononuclear Cells to Lipopolysaccharide in vitro in Patients with Primary Biliary Cirrhosis[J]. Medical Journal of Peking Union Medical College Hospital, 2010, 1(1): 60-65.
Citation: Wei-duan ZHONG, Li WANG, Jie CUI, Feng-chun ZHANG. Immune Response of Peripheral Blood Mononuclear Cells to Lipopolysaccharide in vitro in Patients with Primary Biliary Cirrhosis[J]. Medical Journal of Peking Union Medical College Hospital, 2010, 1(1): 60-65.
shu

Immune Response of Peripheral Blood Mononuclear Cells to Lipopolysaccharide in vitro in Patients with Primary Biliary Cirrhosis

  • Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100032, China

More Information
  • Corresponding author:

    ZHANG Feng-chun Tel: 010-88068792, E-mail:zhangfccra@yahoo.com.cn

  • Received Date: March 08, 2010
  • Issue Publish Date: July 29, 2010
    Graphical Abstract
    • Abstract
  •   Objective  To explore the roles of lipopolysaccharide (LPS) and cytokines in the pathogenesis of primary biliary cirrhosis (PBC).
      Methods  The peripheral blood mononuclear cells (PBMCs) were collected from PBC patients, patients with primary Sjögren's syndrome (pSS), and healthy controls and then cultured in vitro in RPMI 1640 medium with or without LPS. The immune responses of PBMCs to 0.01mg/ml LPS in vitro were investigated by measuring the levels of cytokines(IL-1β, IL-2, IL-6, IL-10, TNF-α, IFN-γ) in supernates using enzyme-linked immunosorbent assay (ELISA).
      Results  The response of freshly extracted PBMCs to LPS were remarkably different from that of the resuscitated PBMCs. In the supernates of PBMCs cultured without LPS for 24 hours in vitro, the level of IL-2 was significantly higher in PBC patients than in healthy controls (P < 0.05). In the supernates of PBMCs cultured with 0.01mg/ml LPS for 24 hours in vitro, the levels of IL-1β, IL-2, and TNF-α were significantly higher in PBC patients than in healthy controls (P < 0.05). After the stimulation of LPS, the elevation rate of IL-1β and decrease rate of IL-2 in PBC patients were significantly higher than those in healthy controls (P < 0.05). Compared with the pSS patients, the levels of cytokines in PBC patients showed no difference in culture media without LPS, while IL-6 level was significantly increased in the supernates cultured with LPS in PBC patients. IFN-γ were seldomly detected in the the supernates of PBMCs from PBC patients, even after the LPS concentrations were increased to 0.1 and 1.0 mg/ml.
      Conclusions  The occurence of PBC is related to bacterial infections, in which LPS may play an important role. Many cytokines are involved in the pathogenesis of PBC, especially Th1 subtype.
    • FullText(HTML)
    • References (17)
  • [1]
    Wasilenko ST, Mason GE, Mason AL.Primary biliary cirrhosis, bacteria and molecula mimicry:what's the molecule and where's the mimic[J]? Liver Int, 2009, 29:779-782. DOI: 10.1111/j.1478-3231.2009.02051.x
    [2]
    Ballot E, Bandin O, Chazouilleres O, et al.Immune response to lipopolysaccharide in primary biliary cirrhosis and autoimmune diseases[J].J Autoimmun, 2004, 22:153- 158. DOI: 10.1016/j.jaut.2003.11.002
    [3]
    Heathcote EJ.Management of primary biliary cirrhosis[J]. Hepatology, 2000, 31:1005-1013. DOI: 10.1053/he.2000.5984
    [4]
    Vitali C, Bombardieoi S, Jonssin R, et al.Classification criteria for Sjögren's syndrome:a revised version of European criteria proposed by the American European Consensus Group[J].Ann Rheum Dis, 2002, 61:554-558. DOI: 10.1136/ard.61.6.554
    [5]
    Brown SB, Savill J.Pagocytosis triggers macrophage release of Fas ligand and induces apoptosis of bystander leukocytes [J].J Immunol, 1999, 162:480-485. http://europepmc.org/abstract/med/9886423
    [6]
    Nagano T, Yamamoto K, Matsumoto S, et al.Cytokine profile in the liver of primary biliary cirrhosis[J].J Clin Immunol, 1999, 19:422-427. DOI: 10.1023/A:1020511002025
    [7]
    Solís Herruzo JA, Solís Muñoz P, Muñoz Yagǜe T.The pathogenesis of primary biliary cirrhosis[J].Rev Esp Enferm Dig, 2009, 101:413-423.
    [8]
    Mao TK, Lian ZX, Selmi C, et al.Altered monocytes responses to defined TLR ligands in patients with primary biliary cirrhosis[J].Hepatology, 2005, 42:802-808. DOI: 10.1002/hep.20859
    [9]
    Barak V, Selmi C, Schlesinger M, et al.Serum inflammatory cytokines, complement components, and soluble interleukin 2 receptor in primary biliary cirrhosis[J].J Autoimmun, 2009, 33:178-182. DOI: 10.1016/j.jaut.2009.09.010
    [10]
    Parikh-Patel A, Gold EB, Worman H, et al.Risk factors for primary biliary cirrhosis in a cohort of patients from the United States[J].Hepatology, 2001, 33:16-21. DOI: 10.1053/jhep.2001.21165
    [11]
    Sasatomi K, Noguchi K, Sakisaka S, et al.Abnormal accumulation of endotoxin in biliary epithelial cells inprimary biliary cirrhosis and primary sclerosing cholangitis[J].J Hepatol, 1998, 29:409-416. DOI: 10.1016/S0168-8278(98)80058-5
    [12]
    Honda Y, Yamagiwa S, Matsuda Y, et al.Altered expression of TLR homolog RP105 on monocytes hypersensitive to LPS in patients with primary biliary cirrhosis[J].J Hepatol, 2007, 47:404-411. DOI: 10.1016/j.jhep.2007.03.012
    [13]
    Dumoulin FL, Leifeld L, Honecker U, et al.Intrahepatic expression of interleukin-1 beta and tumor necrosis factor-alpha in chronic hepatitis C [J].J Infect Dis, 1999, 180: 1704-1708. DOI: 10.1086/315070
    [14]
    Yamashiki M, Kosaka Y, Nishimura A, et al.Analysis of serum Cytokine Levels in primary biliary cirrhosis patients and healthy adults[J].J Clin Lab Anal, 1998, 12:77-82. DOI: 10.1002/(SICI)1098-2825(1998)12:2<77::AID-JCLA1>3.0.CO;2-G
    [15]
    Lohr HF, Schlaak JF, Gerken G, et al.Phenotypic alanalysis and cytokine release of liver-infiltrating and peripheral blood T lymphocytes from patients with chronic hepatitis of different etiology[J].Liver, 1994, 14:161-166. http://www.ncbi.nlm.nih.gov/pubmed/8078396/
    [16]
    Matsumoto T, Morizane T, Aoki Y, et al.Autoimmune hepatitis in primary Sjögren's Syndrome:pathological study of the livers and labial salivary glands in 17 patients with primary Sjögren's Syndrome[J].Pathol Int, 2005, 55:70- 76. DOI: 10.1111/j.1440-1827.2005.01790.x
    [17]
    Liu B, Zhang FC, Zhang ZL, et al.Interstitial lung disease and Sjögren's syndrome in primary biliary cirrhosis:a causal or casual association [J]? Clin Rheumatol, 2008, 27: 1299-1306. DOI: 10.1007/s10067-008-0917-x
    • Related Articles

  • Related Articles

    [1]ZHU He, WEN Bei, XU Li, HUANG Yuguang. Mechanism of Wnt5a on Keratinocyte Regulating MMP9 for CRPS-Ⅰ Peripheral Sensitization[J]. Medical Journal of Peking Union Medical College Hospital, 2024, 15(2): 335-343. DOI: 10.12290/xhyxzz.2023-0551
    [2]XIE Lantian, RUAN Guiren, LIU Xiaoqing, ZHANG Lifan, ZHANG Yueqiu, QIU Zhifeng, SHI Xiaochun. Changes of Peripheral Blood Lymphocyte Subsets in 212 Active Tuberculosis Patients: A Uni-centered Descriptive Study[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(1): 131-138. DOI: 10.12290/xhyxzz.2022-0645
    [3]XIAO Nan, LI Zhanfeng, YAO Jianxin, PAN Zhiyao, YAO Zhifeng. Long Non-coding RNA and Cancer Stem Cells[J]. Medical Journal of Peking Union Medical College Hospital, 2021, 12(3): 373-379. DOI: 10.12290/xhyxzz.20190231
    [4]Teng ZHU, Hong-zhong JIN. mRNA Expression of Methylase and Methyl-CpG Binding Protein in Peripheral Blood Mononuclear Cells from Patients with Generalized Pustular Psoriasis[J]. Medical Journal of Peking Union Medical College Hospital, 2019, 10(4): 358-363. DOI: 10.3969/j.issn.1674-9081.2019.04.009
    [5]Hong Yang, Shanshan Hu, Gechong Ruan, Xiaoou Yang, Jiaming Qian. Expression of ARHI and HuR in PANC-1 Cells[J]. Medical Journal of Peking Union Medical College Hospital, 2017, 8(1): 39-42. DOI: 10.3969/j.issn.1674-9081.2017.01.009
    [6]Zhong-juan LIU, Gui-yu SONG, Zhao-xia ZHAO, Lin ZHANG, Xu-zhen QIN, Rui-li ZHANG, Hui-juan HAN, Zi-jian GUO. Effect of CA125 on the Cellular Immune Function in Patients with Ovarian Cancer[J]. Medical Journal of Peking Union Medical College Hospital, 2014, 5(3): 273-277. DOI: 10.3969/j.issn.1674-9081.2014.03.006
    [7]Gao ZENG, Peng-ran SUN, An-lei LIU, Jie LIU, Shu-bin GUO. Expressions of Lipocalin 2 and Its Relative Cytokines in Endotoxic Mice[J]. Medical Journal of Peking Union Medical College Hospital, 2013, 4(3): 260-264. DOI: 10.3969/j.issn.1674-9081.2013.03.008
    [8]Jun LI, Lin-na WANG, He-yi ZHENG. Levels of Interleukin-2 in Peripheral Blood of Patients with Secondary Syphilis[J]. Medical Journal of Peking Union Medical College Hospital, 2012, 3(4): 395-397. DOI: 10.3969/j.issn.1674-9081.2012.04.007
    [9]Xiao-hua SHI, Zhi-yong LIANG, Huan-wen WU, Xin-yu REN, Tong-hua LIU. Effect of RNA Interference Plasmid on the Expression of Oncogene AKT2 in Pancreatic Cancer Cell Line Panc-1[J]. Medical Journal of Peking Union Medical College Hospital, 2012, 3(1): 102-108. DOI: 10.3969/j.issn.1674-9081.2012.01.021
    [10]Xi LI, Dan-xu MA, Lei ZHANG, Ting ZHANG, Li WANG, Qun SHI, Qian WANG, Qing-jun WU, Xuan ZHANG, Feng-chun ZHANG, Yong-zhe LI. Expression of Perforin in the Peripheral Blood of Patients with Primary Biliary Cirrhosis and Its Clinical Significance[J]. Medical Journal of Peking Union Medical College Hospital, 2010, 1(2): 125-131.

  • Cited by

    Periodical cited type(6)

    1. 王德礼，张颖，徐烨，周效竹，崔向丽，陈世财. 基于WOS数据库药师参与抗菌药物管理的文献计量和可视化分析. 中国医院用药评价与分析. 2024(09): 1054-1058 .
    2. 陈晓瑾，王萍萍，廖莉，陈俊松，林先耀，周红萍. 某儿童医院联合关键绩效指标的抗菌药物精准管理实践. 中华医院管理杂志. 2023(05): 378-382 .
    3. 马莉，冯变玲，王金萍，韩小年，昝鑫，尚秦玉，彭莉蓉. PDCA循环法用于碳青霉烯类抗菌药物多学科协作管理项目的效果分析. 中国药业. 2021(17): 15-17 .
    4. 赵多爱，赵晓燕，周蕾，方晓燕，杨海燕，高婷. 金昌市女性盆腔炎病原菌谱及耐药性分析. 国际检验医学杂志. 2020(16): 2024-2027 .
    5. 朱珠，张波，李乃适，唐彦，梅丹，李大魁. 1927年版北京协和医院处方集撷要. 协和医学杂志. 2020(05): 631-637 . 本站查看
    6. 林梅英，刘益峰. 经皮冠状动脉介入术使用抗菌药物临床药师干预效果分析. 人民军医. 2019(12): 1172-1175 .

    Other cited types(2)

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (152) PDF downloads (13) Cited by(8)
    Related
    Links: National Health Commission of the People's Republic of China Chinese Academy of Medical Sciences(CAMS) AMLC Check CNKI Database CNKI Term Online Chinese General Practice
    Address：Room 302,Third Floor,Building 7, No. 1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing 100730, China
    Tel：010-69154261/4262
    E - mail：medj@pumch.cn mjpumch@126.com
    Copyright of website：Editorial Office of Medical Journal of Peking Union Medical College Hospital

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return
    x Close Forever Close