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Jul.  2010
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Wei-duan ZHONG, Li WANG, Jie CUI, Feng-chun ZHANG. Immune Response of Peripheral Blood Mononuclear Cells to Lipopolysaccharide in vitro in Patients with Primary Biliary Cirrhosis[J]. Medical Journal of Peking Union Medical College Hospital, 2010, 1(1): 60-65.
Citation: Wei-duan ZHONG, Li WANG, Jie CUI, Feng-chun ZHANG. Immune Response of Peripheral Blood Mononuclear Cells to Lipopolysaccharide in vitro in Patients with Primary Biliary Cirrhosis[J]. Medical Journal of Peking Union Medical College Hospital, 2010, 1(1): 60-65.
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Immune Response of Peripheral Blood Mononuclear Cells to Lipopolysaccharide in vitro in Patients with Primary Biliary Cirrhosis

  • Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100032, China

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  • Corresponding author:

    ZHANG Feng-chun Tel: 010-88068792, E-mail:zhangfccra@yahoo.com.cn

  • Received Date: March 08, 2010
  • Issue Publish Date: July 29, 2010
    Graphical Abstract
    • Abstract
  •   Objective  To explore the roles of lipopolysaccharide (LPS) and cytokines in the pathogenesis of primary biliary cirrhosis (PBC).
      Methods  The peripheral blood mononuclear cells (PBMCs) were collected from PBC patients, patients with primary Sjögren's syndrome (pSS), and healthy controls and then cultured in vitro in RPMI 1640 medium with or without LPS. The immune responses of PBMCs to 0.01mg/ml LPS in vitro were investigated by measuring the levels of cytokines(IL-1β, IL-2, IL-6, IL-10, TNF-α, IFN-γ) in supernates using enzyme-linked immunosorbent assay (ELISA).
      Results  The response of freshly extracted PBMCs to LPS were remarkably different from that of the resuscitated PBMCs. In the supernates of PBMCs cultured without LPS for 24 hours in vitro, the level of IL-2 was significantly higher in PBC patients than in healthy controls (P < 0.05). In the supernates of PBMCs cultured with 0.01mg/ml LPS for 24 hours in vitro, the levels of IL-1β, IL-2, and TNF-α were significantly higher in PBC patients than in healthy controls (P < 0.05). After the stimulation of LPS, the elevation rate of IL-1β and decrease rate of IL-2 in PBC patients were significantly higher than those in healthy controls (P < 0.05). Compared with the pSS patients, the levels of cytokines in PBC patients showed no difference in culture media without LPS, while IL-6 level was significantly increased in the supernates cultured with LPS in PBC patients. IFN-γ were seldomly detected in the the supernates of PBMCs from PBC patients, even after the LPS concentrations were increased to 0.1 and 1.0 mg/ml.
      Conclusions  The occurence of PBC is related to bacterial infections, in which LPS may play an important role. Many cytokines are involved in the pathogenesis of PBC, especially Th1 subtype.
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    • References (17)
  • [1]
    Wasilenko ST, Mason GE, Mason AL.Primary biliary cirrhosis, bacteria and molecula mimicry:what's the molecule and where's the mimic[J]? Liver Int, 2009, 29:779-782. DOI: 10.1111/j.1478-3231.2009.02051.x
    [2]
    Ballot E, Bandin O, Chazouilleres O, et al.Immune response to lipopolysaccharide in primary biliary cirrhosis and autoimmune diseases[J].J Autoimmun, 2004, 22:153- 158. DOI: 10.1016/j.jaut.2003.11.002
    [3]
    Heathcote EJ.Management of primary biliary cirrhosis[J]. Hepatology, 2000, 31:1005-1013. DOI: 10.1053/he.2000.5984
    [4]
    Vitali C, Bombardieoi S, Jonssin R, et al.Classification criteria for Sjögren's syndrome:a revised version of European criteria proposed by the American European Consensus Group[J].Ann Rheum Dis, 2002, 61:554-558. DOI: 10.1136/ard.61.6.554
    [5]
    Brown SB, Savill J.Pagocytosis triggers macrophage release of Fas ligand and induces apoptosis of bystander leukocytes [J].J Immunol, 1999, 162:480-485. http://europepmc.org/abstract/med/9886423
    [6]
    Nagano T, Yamamoto K, Matsumoto S, et al.Cytokine profile in the liver of primary biliary cirrhosis[J].J Clin Immunol, 1999, 19:422-427. DOI: 10.1023/A:1020511002025
    [7]
    Solís Herruzo JA, Solís Muñoz P, Muñoz Yagǜe T.The pathogenesis of primary biliary cirrhosis[J].Rev Esp Enferm Dig, 2009, 101:413-423.
    [8]
    Mao TK, Lian ZX, Selmi C, et al.Altered monocytes responses to defined TLR ligands in patients with primary biliary cirrhosis[J].Hepatology, 2005, 42:802-808. DOI: 10.1002/hep.20859
    [9]
    Barak V, Selmi C, Schlesinger M, et al.Serum inflammatory cytokines, complement components, and soluble interleukin 2 receptor in primary biliary cirrhosis[J].J Autoimmun, 2009, 33:178-182. DOI: 10.1016/j.jaut.2009.09.010
    [10]
    Parikh-Patel A, Gold EB, Worman H, et al.Risk factors for primary biliary cirrhosis in a cohort of patients from the United States[J].Hepatology, 2001, 33:16-21. DOI: 10.1053/jhep.2001.21165
    [11]
    Sasatomi K, Noguchi K, Sakisaka S, et al.Abnormal accumulation of endotoxin in biliary epithelial cells inprimary biliary cirrhosis and primary sclerosing cholangitis[J].J Hepatol, 1998, 29:409-416. DOI: 10.1016/S0168-8278(98)80058-5
    [12]
    Honda Y, Yamagiwa S, Matsuda Y, et al.Altered expression of TLR homolog RP105 on monocytes hypersensitive to LPS in patients with primary biliary cirrhosis[J].J Hepatol, 2007, 47:404-411. DOI: 10.1016/j.jhep.2007.03.012
    [13]
    Dumoulin FL, Leifeld L, Honecker U, et al.Intrahepatic expression of interleukin-1 beta and tumor necrosis factor-alpha in chronic hepatitis C [J].J Infect Dis, 1999, 180: 1704-1708. DOI: 10.1086/315070
    [14]
    Yamashiki M, Kosaka Y, Nishimura A, et al.Analysis of serum Cytokine Levels in primary biliary cirrhosis patients and healthy adults[J].J Clin Lab Anal, 1998, 12:77-82. DOI: 10.1002/(SICI)1098-2825(1998)12:2<77::AID-JCLA1>3.0.CO;2-G
    [15]
    Lohr HF, Schlaak JF, Gerken G, et al.Phenotypic alanalysis and cytokine release of liver-infiltrating and peripheral blood T lymphocytes from patients with chronic hepatitis of different etiology[J].Liver, 1994, 14:161-166. http://www.ncbi.nlm.nih.gov/pubmed/8078396/
    [16]
    Matsumoto T, Morizane T, Aoki Y, et al.Autoimmune hepatitis in primary Sjögren's Syndrome:pathological study of the livers and labial salivary glands in 17 patients with primary Sjögren's Syndrome[J].Pathol Int, 2005, 55:70- 76. DOI: 10.1111/j.1440-1827.2005.01790.x
    [17]
    Liu B, Zhang FC, Zhang ZL, et al.Interstitial lung disease and Sjögren's syndrome in primary biliary cirrhosis:a causal or casual association [J]? Clin Rheumatol, 2008, 27: 1299-1306. DOI: 10.1007/s10067-008-0917-x
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