Email Alert RSS
Volume 1 Issue 2
Oct.  2010
Turn off MathJax
Article Contents
Abstract
References
Related Articles
Zheng MIAO, Jia-ming QIAN, Jing-nan LI, Xiao-yan TANG, Xiao-ou YANG. Establishment of Two Murine Models of Ulcerative Colitis and Comparison of the Mechanism of Colitis-related Inflammatory Reactions[J]. Medical Journal of Peking Union Medical College Hospital, 2010, 1(2): 145-149.
Citation: Zheng MIAO, Jia-ming QIAN, Jing-nan LI, Xiao-yan TANG, Xiao-ou YANG. Establishment of Two Murine Models of Ulcerative Colitis and Comparison of the Mechanism of Colitis-related Inflammatory Reactions[J]. Medical Journal of Peking Union Medical College Hospital, 2010, 1(2): 145-149.
shu

Establishment of Two Murine Models of Ulcerative Colitis and Comparison of the Mechanism of Colitis-related Inflammatory Reactions

  • Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100730, China

More Information
  • Corresponding author:

    LI Jing-nan Tel:010-65295262, E-mail:lijn2008@gmail.com

  • Received Date: July 29, 2010
  • Issue Publish Date: October 29, 2010
    Graphical Abstract
    • Abstract
  •   Objective  To establish two murine models of ulcerative colitis (UC) and explore the mechanism of colitits-related inflammatory reactions.
      Methods  Totally 12 C57BL mice were randomly divided into two groups:dextransulfatesodium (DSS) model group (n=8, allowed to drink 3% DSS for 5 days) and DSS control group (n=4, allowed to drink water for 5 days). In addition, 8 BALB/C mice were randomly divided into oxazolone (OXZ) model group(n=4, mice were skin-sensitized with 3% OXZ 0.2 ml in 100% ethanol for 2 days followed by intrarectal administration of 0.8% OXZ 0.15 ml in 50% ethanol 5 days later) and OXZ control group(n=4, mice were skin-sensitized with 100% ethanol for 2 days followed by intrarectal administration of 50% ethanol 0.15 ml 5 days later). The disease activity index (DAI), tissular general score, and histological score were calculated. The levels of myeloperoxidase (MPO), interleukin 4 (IL-4), interferon-γ (INF-γ), tumor necrosis factor-α(TNF-α), and nuclear factor-Kappa B (NF-κB) in the colon tissue were determined.
      Results  DAI, tissular general score, and histological score were significantly different in either DSS or OXZ model group when compared with their control groups. MPO, TNF-α, and NF-κB significantly increased in DSS and OXZ model groups. In addition, INF-γ significantly increased in DSS model group and IL-4 significantly increased in OXZ model group.
      Conclusions  Both DSS and OXZ can induce ulcerative colitis in mice. OXZ-induced murine colitis is highly associated with T helper cell type 2 (Th2), which is more similar to human UC.
    • FullText(HTML)
    • References (13)
  • [1]
    Abraham C, Cho JH.Inflammatory bowel disease[J]. New Engl J Med, 2009, 361:2066-2078. DOI: 10.1056/NEJMra0804647
    [2]
    Melgar S, Karlsson A, Michaelsson E.Acute colitis induced by dextran sulphate sodium progresses into chronicity in C57BL/6 but not in BALB/c mice-correlation between symptoms and inflammation[J]. Am J Physiol Gastrointest Liver Physiol, 2005, 288:G1328-G1338. DOI: 10.1152/ajpgi.00467.2004
    [3]
    Heller F, Fuss IJ, Nieuwenhuis EE, et al.Oxazolone colitis, a Th2 colitis model resembling ulcerative colitis, is mediated by IL-13 -producing NK-T cells[J]. Immunity, 2002, 17:629-638. DOI: 10.1016/S1074-7613(02)00453-3
    [4]
    Okayasu I, Hatakeyama S, Yamada M, et al. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice[J]. Gastroenterology, 1990, 98:694-702. DOI: 10.1016/0016-5085(90)90290-H
    [5]
    Luk HH, Ko JK, Fung HS, et al.Delineation of the protective action of zinc sulfate on ulcerative colitis in rats[J]. Eur J Pharmacol, 2002, 443:197-204. DOI: 10.1016/S0014-2999(02)01592-3
    [6]
    Ekstrom GM.Oxazolone-induced colitis in rats:effects of budesonide, cyclosporin A, and 5-aminosalicylic acid[J]. Scand J Gastroenterol, 1998, 33:174-179. DOI: 10.1080/00365529850166914
    [7]
    Fausto SM, Aaron DL, Jesus KY.Role of cytokines in inflammatory bowel disease[J]. World Gastroenterol, 2008, 14:4280-4288. DOI: 10.3748/wjg.14.4280
    [8]
    Forbes E, Murase T, Yang M, et al.Immunopathogenesis of experimental ulcerative colitis is mediated by eosinophil peroxidase[J]. J Immunol, 2004, 172:5664-5675. DOI: 10.4049/jimmunol.172.9.5664
    [9]
    Choi SY, Hur SJ, An CS, et al.Anti-inflammatory effects of Inonotus obliquus in colitis induced by dextran sodium sulfate [J]. Biomed Biotechnol, 2010-03-10 [Epub ahead of print]. http://www.hindawi.com/journals/jbb/.
    [10]
    Ryotaro K, Satoko K, Tomiko O, et al.Oxazolone-induced Colitis in BALB/C mice:a new method to evaluate the efficacy of therapeutic agents for ulcerative Colitis[J]. Pharmacol Sci, 2004, 96:307-313. DOI: 10.1254/jphs.FP0040214
    [11]
    Dharmani P, Chadee K.Biologic therapies against inflammatory bowel disease:a dysregulated immune system and the cross talk with gastrointestinal mucosa hold the key[J]. Curr Mol Pharmacol, 2008, 1:195-212. DOI: 10.2174/1874467210801030195
    [12]
    David QS, Stephan RT.Immunopathogenesis of inflammatory bowel disease[J]. World J Gastroenterol, 2008, 14: 390-400. http://doi.med.wanfangdata.com.cn/qk/wjg200803007
    [13]
    Neurath MF, Finotto S, Glimcher LH.The role of Th1/Th2 polarization in mucosal immunity[J]. Nat Med, 2002, 8: 567-573 DOI: 10.1038/nm0602-567
    • Related Articles

  • Related Articles

    [1]ZHANG Lu, LI Jian. Castleman Disease in China: State-of-the-art Technology Before the Era of IL-6 Targeted Therapy[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(5): 911-914. DOI: 10.12290/xhyxzz.2023-0227
    [2]LIU Yanting, ZHANG Lu, CHEN Miao, ZHANG Yan, WEI Chong, PAN Boju, ZHAO Jiawei. A Case of Small B-cell Lymphocytic Lymphoma Diagnosed One Year after Evans Syndrome[J]. Medical Journal of Peking Union Medical College Hospital, 2021, 12(3): 423-426. DOI: 10.12290/xhyxzz.20200063
    [3]TAN Yu-ting, SHI Xiao-chun, LIU Xiao-qing. Testing and Prophylaxis of Latent Tuberculosis Infection in Patients Receiving Tumor Necrosis Factor-α Antagonists[J]. Medical Journal of Peking Union Medical College Hospital, 2021, 12(1): 86-91. DOI: 10.12290/xhyxzz.20180406
    [4]Yuan XIA, Xiang-pei LI. The Role of MicroRNA Regulation of Interferon-α in the Pathogenesis of Systemic Lupus Erythematosus[J]. Medical Journal of Peking Union Medical College Hospital, 2019, 10(6): 673-678. DOI: 10.3969/j.issn.1674-9081.2019.06.021
    [5]Ju QIAO, Feng LI, Chun-xia HE, Chao WU, Hong-zhong JIN. Effects of the Receptor-IgG Fc Fusion Protein of Recombinant Type Ⅱ Human Tumor Necrosis Factor-α on the Levels of Serum Adipokines in Psoriatic Patients: A Prospective Non-randomized Controlled Trial[J]. Medical Journal of Peking Union Medical College Hospital, 2019, 10(3): 243-248. DOI: 10.3969/j.issn.1674-9081.2019.03.010
    [6]Qian CAO. The Crucial Problems in the Treatment of Crohn's Disease with Anti-tumor Necrosis Factor-α Monoclonal Antibody[J]. Medical Journal of Peking Union Medical College Hospital, 2017, 8(4-5): 207-209. DOI: 10.3969/j.issn.1674-9081.2017.05.003
    [8]Ying ZHANG, Jin-song GAO, Jun-tao LIU, Hai-yuan LIU, Ying-na SONG, Xiao-ming GONG, Zhi-jing SUN. Effectiveness of B-lynch Suture in Treatment and Prevention of Postpartum Hemorrhage[J]. Medical Journal of Peking Union Medical College Hospital, 2013, 4(2): 174-177. DOI: 10.3969/j.issn.1674-9081.2013.02.019
    [9]Yan XU, Cai-yan LIU, Yao ZHANG, Ling-yan KONG, Feng FENG, Zheng-yu JIN, Li-ying CUI. Serum Uric Acid Levels in Chinese Patients with Classic Multiple Sclerosis: Before and After Treatment with Interferon-beta 1b[J]. Medical Journal of Peking Union Medical College Hospital, 2012, 3(3): 282-286. DOI: 10.3969/j.issn.1674-9081.2012.03.008
    [10]Lin WANG, Wen ZHANG, Meng-tao LI, Yan ZHAO. Correlation of Memory B Cell and B Cell Activation Factor with Clinical Indicators in Primary Sj?gren's Syndrome Patients and Their First-degree Relatives[J]. Medical Journal of Peking Union Medical College Hospital, 2010, 1(2): 132-136.

  • Cited by

    Periodical cited type(2)

    1. 梁桂丽，李灿美，邢成锋，董运龙，王娟，雷梓，缪应雷，兰丹凤. GC-C~(-/-)小鼠在DSS诱导下肠道炎症损伤的变化. 昆明医科大学学报. 2022(07): 1-8 .
    2. 孔红梅，程毅东. 复方谷氨酰胺联合益生菌对活动期溃疡性结肠炎肠道屏障功能及细胞因子的影响. 实用临床医药杂志. 2014(13): 29-32 .

    Other cited types(12)

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (981) PDF downloads (90) Cited by(14)
    Related
    Links: National Health Commission of the People's Republic of China Chinese Academy of Medical Sciences(CAMS) AMLC Check CNKI Database CNKI Term Online Chinese General Practice
    Address：Room 302,Third Floor,Building 7, No. 1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing 100730, China
    Tel：010-69154261/4262
    E - mail：medj@pumch.cn mjpumch@126.com
    Copyright of website：Editorial Office of Medical Journal of Peking Union Medical College Hospital

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return
    x Close Forever Close