Email Alert RSS
Volume 11 Issue 2
Mar.  2020
Turn off MathJax
Article Contents
Abstract
References
Related Articles
Ying LI, Ge ZHANG, Yang YANG, Dong-xue LI, He WANG, Ying-chun XU. Evaluation of Influence Factors in the Identification of Clinical Filamentous Fungi by Matrix Assisted Laser Desorption Ionization-time of Flight Mass Spectrometry[J]. Medical Journal of Peking Union Medical College Hospital, 2020, 11(2): 156-161. DOI: 10.3969/j.issn.1674-9081.20170245
Citation: Ying LI, Ge ZHANG, Yang YANG, Dong-xue LI, He WANG, Ying-chun XU. Evaluation of Influence Factors in the Identification of Clinical Filamentous Fungi by Matrix Assisted Laser Desorption Ionization-time of Flight Mass Spectrometry[J]. Medical Journal of Peking Union Medical College Hospital, 2020, 11(2): 156-161. DOI: 10.3969/j.issn.1674-9081.20170245
shu

Evaluation of Influence Factors in the Identification of Clinical Filamentous Fungi by Matrix Assisted Laser Desorption Ionization-time of Flight Mass Spectrometry

  • 1.

    Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China

  • 2.

    Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China

More Information
  • Corresponding author:

    XU Ying-chun Tel: 86-10-69159766, E-mail: xycpumch@139.com

  • Received Date: November 06, 2017
  • Issue Publish Date: March 29, 2020
    Graphical Abstract
    • Abstract
  •   Objective  This study aimed to evaluate the potential influence of culture conditions, incubation time, and the interpreting cut-off value(COV) in the identification of filamentous fungi by matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS).
      Methods  Seventy-eight isolates of filamentous fungi were cultivated in sabouraud dextrose agar(SDA) or potato dextrose agar(PDA) media with an incubation temperature of 28℃ or 35℃ separately, namely four different culture conditions.MALDI-TOF MS identification was carried out at different time including the 2nd, 3rd, 4th, 5th and 7th days of incubation of the isolates. Differences in identification capacities of MALDI-TOF MS under the tested parameters were analyzed to determine the optimal culture condition and incubation time of filamentous fungi. The influence of lowered interpreting COV in MALDI-TOF MS identification of filamentous fungi was also evaluated.
      Results  No significant difference was detected between the SDA and PDA culture media in the identification capacities of MALDI-TOF MS(χ2=0.467, P=0.792). The identification capacity of MALDI-TOF MS with the incubation temperature of 28℃ was superior to that of 35℃(χ2=7.195, P=0.027). Higher identification rates of species level(40.3%, 34.3%) and genus level(44.8%, 46.3%) were achieved in isolates incubated for 2 and 3 days. The identification rate of species level was increased significantly from 32.1% to 85.9%(χ2=40.119, P < 0.01) without increasing the false identification rate after adjusting the interpreting COV from score > 2.0 to > 1.7.
      Conclusion  The performance of MALDI-TOF MS in the identification of filamentous fungi could be improved by applying the optimal culture condition of incubating in SDA or PDA media under the temperature of 28℃ for 2 to 3 days and by adjusting the interpreting COV of the species level with a score > 1.7.
    • FullText(HTML)
    • References (16)
  • [1]
    Taccone FS, Van den Abeele AM, Bulpa P, et al. Epidemiology of invasive aspergillosis in critically ill patients:clinical presentation, underlying conditions, and outcomes[J]. Crit Care, 2015, 19:7. DOI: 10.1186/s13054-014-0722-7
    [2]
    Liao Y, Chen M, Hartmann T, et al. Epidemiology of opportunistic invasive fungal infections in China:review of literature[J]. Chin Med J(Engl), 2013, 126:361-368. http://europepmc.org/abstract/med/23324290
    [3]
    Slavin M, van Hal S, Sorrell TC, et al. Invasive infections due to filamentous fungi other than Aspergillus:epidemiology and determinants of mortality[J]. Clin Microbiol Infect, 2015, 21:490.e1-e10. DOI: 10.1016/j.cmi.2014.12.021
    [4]
    Blum G, Hörtnagl C, Jukic E, Erbeznik T, et al. New insight into amphotericin B resistance in Aspergillus terreus[J]. Antimicrob Agents Chemother, 2013, 57:1583-1588. DOI: 10.1128/AAC.01283-12
    [5]
    Martos AI, Romero A, González MT, et al. Evaluation of the Etest method for susceptibility testing of Aspergillus spp. and Fusarium spp. to three echinocandins[J]. Med Mycol, 2010, 48:858-861. DOI: 10.3109/13693781003586943
    [6]
    Alastruey-Izquierdo A, Castelli MV, Cuesta I, et al. In vitro activity of antifungals against Zygomycetes[J]. Clin Microbiol Infect, 2009, 15:71-76. DOI: 10.1111/j.1469-0691.2009.02984.x
    [7]
    Halliday CL, Chen SC, Kidd SE, et al. Antifungal susceptibilities of non-Aspergillus filamentous fungi causing invasive infection in Australia:support for current antifungal guide-line recommendations[J]. Int J Antimicrob Agents, 2016, 48:453-458. DOI: 10.1016/j.ijantimicag.2016.07.005
    [8]
    Singhal N, Kumar M, Kanaujia PK, et al. MALDI-TOF mass spectrometry:an emerging technology for microbial identification and diagnosis[J]. Front Microbiol, 2015, 6:791. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525378/
    [9]
    Cassagne C, Normand AC, L'Ollivier C, et al. Performance of MALDI-TOF MS platforms for fungal identification[J]. Mycoses, 2016, 59:678-690. DOI: 10.1111/myc.12506
    [10]
    Schulthess B, Ledermann R, Mouttet F, et al. Use of the Bruker MALDI Biotyper for identification of molds in the clinical mycology laboratory[J]. J Clin Microbiol, 2014, 52:2797-2803. DOI: 10.1128/JCM.00049-14
    [11]
    Lau AF, Drake SK, Calhoun LB, et al. Development of a clinically comprehensive database and a simple procedure for identification of molds from solid media by MALDI-TOF MS[J]. J Clin Microbiol, 2013, 51:828-834. DOI: 10.1128/JCM.02852-12
    [12]
    Li Y, Wang H, Zhao YP, et al. Evaluation of the Bruker Biotyper Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry system for identification of Aspergillus species directly from growth on solid agar media[J]. Front Microbiol, 2017, 8:1209. DOI: 10.3389/fmicb.2017.01209
    [13]
    李颖, 徐英春.评价ITS、BenA和CaM序列分析在曲霉菌种鉴定方面的应用[J].中国真菌学杂志, 2017, 12:74-77. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgzjxzz201702003
    [14]
    李颖, 郭莉娜, 徐英春.内转录间隔区(ITS)序列分析技术对丝状真菌临床分离株鉴定能力的评估[J].中华微生物学和免疫学杂志, 2017, 37:607-610. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zhwswxhmyx201708010
    [15]
    Alanio A, Beretti JL, Dauphin B, et al. MALDI-TOF MS for fast and accurate identification of clinically relevant Aspergillus species[J]. Clin Microbiol Infect, 2011, 17:750-755. DOI: 10.1111/j.1469-0691.2010.03323.x
    [16]
    Samson RA, Visagie CM, Houbraken J, et al. Phylogeny, identification and nomenclature of the genus Aspergillus[J]. Stud Mycol, 2014, 78:141-173. DOI: 10.1016/j.simyco.2014.07.004
    • Related Articles

  • Related Articles

    [1]WANG Yutong, ZHU Weiguo, SUN Xueqin, TONG Jiali, ZHOU Jingya, ZHAO Qing, LI Bocheng, ZHANG Wei, LIU Xiaokun, DONG Rui, XIE Chen, HAN Ding. Factors Influencing Inpatient Costs for Patients Undergoing Surgery for Intrauterine Lesions under DRG Payment[J]. Medical Journal of Peking Union Medical College Hospital, 2024, 15(5): 1069-1076. DOI: 10.12290/xhyxzz.2024-0375
    [2]YAN Xinchun, HUO Li. Evaluation of Von Hippel-Lindau Syndrome Through Novel Small Molecular Tracer 68Ga-NY104 PET/CT Imaging[J]. Medical Journal of Peking Union Medical College Hospital, 2024, 15(4): 911-915. DOI: 10.12290/xhyxzz.2024-0216
    [3]DAO Riao, TIAN Yuan, CHEN Huan, ZHANG Yuelun, YU Chunhua. Incidence and Influencing Factors of Subacute and Chronic Pain in Patients Returning to ICU After Surgery[J]. Medical Journal of Peking Union Medical College Hospital, 2024, 15(3): 598-603. DOI: 10.12290/xhyxzz.2023-0580
    [4]WANG Xiaodan, ZHANG Zhan, LIU Zhaohui. Analysis of Influencing Factors for High-risk Human Papillomavirus Infection[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(1): 153-158. DOI: 10.12290/xhyxzz.2022-0193
    [5]FU Jianlei, SU Longxiang, PAN Wenjun, LIN Guoying, CHAI Wenzhao, WANG Xiaoting. Analysis of the Situation and Influencing Factors of Fungal Detection in Adult Patients with Severe Diseases in Tibet Region[J]. Medical Journal of Peking Union Medical College Hospital, 2021, 12(5): 791-797. DOI: 10.12290/xhyxzz.2021-0531
    [7]Zi-jian GUO, Zhong-juan LIU, Rui-li ZHANG, Hui-juan HAN, Xu-zhen QIN, Li ZHANG, Tao LIU, Xue LI, Yu XIAO, Qian LIU, Liang-yu XIA, Xin-qi CHENG. Diagnostic Value of Serum Pro-gastrin-releasing Peptide and Neuron-specific Enolase for Small Cell Lung Cancer and Its Influencing Factors[J]. Medical Journal of Peking Union Medical College Hospital, 2014, 5(3): 259-263. DOI: 10.3969/j.issn.1674-9081.2014.03.003
    [8]Hui-ming PENG, Xi-sheng WENG, Ji-liang ZHAI, Jin LIN, Jin JIN, Wen-wei QIAN, Li-juan ZHAO. Allogeneic Transfusion Rate and Related Factors in Primary Total Knee Arthroplasty[J]. Medical Journal of Peking Union Medical College Hospital, 2014, 5(2): 135-141. DOI: 10.3969/j.issn.1674-9081.2014.02.002
    [9]Jing-hu SUI, Ye WANG, Xiao-wen LIU, Lei WANG, Dong YANG, Ling-xin WEI, Xiao-ming DENG. Effects of Different Manual Airway Maneuvers on Bonfils Intubation Fiberscope-guided Tracheal Intubation[J]. Medical Journal of Peking Union Medical College Hospital, 2011, 2(4): 338-342. DOI: 10.3969/j.issn.1674-9081.2011.04.011
    [10]Jing-ran ZHEN, Cai-hong MA, Mei-jing XIE, Qi YU, Cheng-yan DENG, Fang-fang HE, Gui-an CHEN, Dong-zi YANG. Vaginal Fungal Infection and Its Influencing Factors during In Vitro Fertilization-embryo Transfer Cycles:A Multicenter Open-label Study[J]. Medical Journal of Peking Union Medical College Hospital, 2011, 2(2): 139-142. DOI: 10.3969/j.issn.1674-9081.2011.02.010

  • Cited by

    Periodical cited type(7)

    1. 蒲川成，冉学军，覃勇志，余江. 同期和分期全膝关节置换术治疗双侧膝骨关节炎的比较. 骨科. 2017(04): 278-282 .
    2. 叶茂，邹毅，张玉峰，桂鹏，冷华伟，张均泉. 同期与分期全膝关节置换术对双侧膝关节重度骨关节炎患者HSS、VAS评分的影响. 齐齐哈尔医学院学报. 2017(23): 2784-2785 .
    3. 韩晓鹏，王小铁. 双侧全膝关节置换同一住院周期分期和一期手术的对比研究. 临床医药实践. 2016(03): 176-178 .
    4. 宝音，庞奕晖，段丽军，葛茂林，李大文，白杰. 双膝关节骨性关节炎同期置换与分期置换的171例对比分析. 武警后勤学院学报(医学版). 2016(09): 737-739 .
    5. 李小鑫. 同期双侧全膝关节置换治疗膝骨关节炎临床研究. 现代预防医学. 2015(06): 1144-1146 .
    6. 李伟，张世权，刘安庆，郝敏，谭纪锋，贺艳，陈幕芬. 双侧一期人工膝关节置换术临床疗效分析. 西部医学. 2014(08): 1011-1013 .
    7. 戴金良，马健，王洪彬，康立新. 同期与分期双侧全膝关节置换术的回顾性研究. 中华关节外科杂志(电子版). 2014(06): 723-725 .

    Other cited types(6)

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (497) PDF downloads (143) Cited by(13)
    Related
    Links: National Health Commission of the People's Republic of China Chinese Academy of Medical Sciences(CAMS) AMLC Check CNKI Database CNKI Term Online Chinese General Practice
    Address：Room 302,Third Floor,Building 7, No. 1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing 100730, China
    Tel：010-69154261/4262
    E - mail：medj@pumch.cn mjpumch@126.com
    Copyright of website：Editorial Office of Medical Journal of Peking Union Medical College Hospital

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return
    x Close Forever Close