Volume 5 Issue 3
Jul.  2014
Turn off MathJax
Article Contents
Juan DU, Jia XU, Ye GUO, Feng ZHANG, Wei WU, Jian-ying LI, Yue LI, Jing JIN, Min CHENG, Wei CUI. Clinical Application of A Fully-automatic Urine Sediment Analyzer[J]. Medical Journal of Peking Union Medical College Hospital, 2014, 5(3): 290-296. doi: 10.3969/j.issn.1674-9081.2014.03.009
Citation: Juan DU, Jia XU, Ye GUO, Feng ZHANG, Wei WU, Jian-ying LI, Yue LI, Jing JIN, Min CHENG, Wei CUI. Clinical Application of A Fully-automatic Urine Sediment Analyzer[J]. Medical Journal of Peking Union Medical College Hospital, 2014, 5(3): 290-296. doi: 10.3969/j.issn.1674-9081.2014.03.009

Clinical Application of A Fully-automatic Urine Sediment Analyzer

doi: 10.3969/j.issn.1674-9081.2014.03.009
More Information
  • Corresponding author: CUI Wei Tel: 010-69159713, E-mail:cuiw@pumch.cn
  • Received Date: 2014-05-04
  • Publish Date: 2014-07-30
  •   Objective  To evaluate the clinical application of a fully-automatic urine sediment analyzer and establish the reference range of urinary formed elements.  Methods  The precision, carryover rate, analytical measurement range, and comparability with manual microscopy method were evaluated, and the reference ranges of red blood cells (RBC), white blood cells (WBC), squamous epithelial cells (EC), and casts by the instrument were analyzed.  Results  The low, medium, and high values of precision were 129.10%, 20.59%, and 7.80% for RBC, and 65.73%, 14.30%, and 13.00% for WBC. The medium and high values of precision for EC were 27.01% and 21.46%. The carryover rate of RBC, WBC, and EC were all 0. The measurement range was 0-83 608/μl for RBC and 0-15 624/μl for WBC. As to the measurement range of bacteria, there were obvious differences according to the different species of bacteria. When compared the instrument results with the manual microscopy, the consistency rates were 82.80% for RBC, 73.82% for WBC, and 57.56% for EC. The reference ranges of urinary formed elements were as follows: the range of urinary RBC was 0-8/μl for males and 0-12/μl for females; the range of urinary WBC was 0-12/μl for males and 0-21/μl for females; the range of urinary EC was 0-13/μl for males and 0-42/μl for females; the range of urinary cast was 0-4/μl for males and 0-4/μl for females; and the range of urinary crystal was 0-34/μl for males and 0-2/μl for females.  Conclusions  This fully-atomatic urine sediment analyzer has better recognition performance for RBC and WBC. We can carry on the manual recognition using audit function by instruments, and manual morphological examination when necessary, to detect crystals, casts, fungi, and irregular cell components which are difficult to identify automatically.
  • loading
  • [1] 熊力凡, 李树仁, 丁磊, 等.临床检验基础[M].3版.北京: 人民卫生出版社, 2003: 62-65.
    [2] Clinical and Laboratory Standards Institute. Precision performance of clinical chemistry devices second edition. EP5-A[S].Wayne, PA: CLSI, 1999.
    [3] 李建英, 陈倩, 史风梅, 等.全自动干化学尿液分析仪及配套试带的临床性能验证[J].中华检验医学杂志, 2012, 35:1112-1117. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zhyxjy201212013
    [4] Dos Santos JC, Weber LP, Perez LR.Evaluation of urinalysis parameters to predict urinary-tract infection[J].Braz J Infect Dis, 2007, 11:479-481. http://europepmc.org/abstract/MED/17962874
    [5] 夏少梅, 林桢, 李婪, 等.泌尿系统感染常见病原菌分布与耐药性分析[J].检验医学与临床, 2009, 19:1603-1605. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jyyxylc200919002
    [6] 陈雨, 程闽, 李薇, 等.自动化尿液干化学和有形成分分析复检规则的制定和应用[J].中华检验医学杂志, 2011, 34:501-506. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zhyxjy201106005
    [7] 任军伟, 陈建魁, 丛王隆, 等.Cobised尿沉渣分析仪与Urisys2400尿液分析仪人工显微镜复查筛选规则的建立[J].现代检验医学杂志, 2013, 28:39-47. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=sxyxjy201302011
    [8] 顾可梁.尿有形成分的识别与检查方法的选择[J].中华检验医学杂志, 2005, 28:572-575. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zhyxjy200506005
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(2)  / Tables(8)

    Article Metrics

    Article views (130) PDF downloads(5) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return