Volume 5 Issue 3
Jul.  2014
Turn off MathJax
Article Contents
Zhong-juan LIU, Gui-yu SONG, Zhao-xia ZHAO, Lin ZHANG, Xu-zhen QIN, Rui-li ZHANG, Hui-juan HAN, Zi-jian GUO. Effect of CA125 on the Cellular Immune Function in Patients with Ovarian Cancer[J]. Medical Journal of Peking Union Medical College Hospital, 2014, 5(3): 273-277. doi: 10.3969/j.issn.1674-9081.2014.03.006
Citation: Zhong-juan LIU, Gui-yu SONG, Zhao-xia ZHAO, Lin ZHANG, Xu-zhen QIN, Rui-li ZHANG, Hui-juan HAN, Zi-jian GUO. Effect of CA125 on the Cellular Immune Function in Patients with Ovarian Cancer[J]. Medical Journal of Peking Union Medical College Hospital, 2014, 5(3): 273-277. doi: 10.3969/j.issn.1674-9081.2014.03.006

Effect of CA125 on the Cellular Immune Function in Patients with Ovarian Cancer

doi: 10.3969/j.issn.1674-9081.2014.03.006
More Information
  • Corresponding author: GUO Zi-jian Tel: 010-69159743, E-mail:guozj@pumch.cn
  • Received Date: 2014-04-04
  • Publish Date: 2014-07-30
  •   Objective  To detect the effects of CA125 on cellular immune function of the ovarian cancer (OC) patients in vitro.  Methods  Totally 58 patients with confirmed OC in our hospital from May 2012 to June 2013 were divided into three groups based on the CA125 levels. Meanwhile, 20 healthy subjects(control group) and 20 patients with benign gynecological diseases (benign diseases group) were also included. The in vitro ability of healthy human lymphocytes in secreting interferon-γ (IFN-γ) under the stimulation of phytohemagglutinin(PHA) was observed by cell culture, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT).  Results  In OC patients, the count and percentage of lymphocytes decreased gradually with elevated levels of CA125. The concentration of IFN-γ in the supernatant of cultured lymphocytes from healthy individuals decreased significantly under the stimulation of PHA and high concentration of CA125[(36.16±16.89)ng/ml]compared with that of PHA alone [(45.68±18.01)ng/ml] or PHA and low concentration of CA125 [(46.22±19.30)ng/ml](both P < 0.05). Compared with the control group(spots number 573.0) and the benign diseases group(spots number 523.0), the high(spots number 108.2) and intermediate(spots number 371.6) concentration of CA125 had significant inhibitory effect on the cellular immune system, as detected by ELISPOT (P < 0.01, P < 0.05, respectively). After removal of CA125 from the serum by antibody, the inhibitory effect was weakened significantly.  Conclusions  The concentration of CA125 in OC patient is negatively correlated with the number and activity of lymphocytes, in particular their abilities in releasing cytokines. CA125 is not only a biomarker of OC but also may serve as an immunosuppressive factor.
  • loading
  • [1] Kobayashi E, Ueda Y, Matsuzaki S, et al. Biomarkers for screening, diagnosis, and monitoring of ovarian cancer[J]. Cancer Epidemiol Biomarkers Prev, 2012, 21:1902-1912. doi:  10.1158/1055-9965.EPI-12-0646
    [2] Rooth C. Ovarian cancer:risk factors, treatment and management[J]. Br J Nurs, 2013, 22:S23-S30. doi:  10.12968/bjon.2013.22.Sup18.S23
    [3] Yin BW, Lloyd KO. Molecular cloning of the CA125 ovarian cancer antigen:identification as a new mucin, MUC16[J]. J Biol Chem, 2001, 276:27371-27375. doi:  10.1074/jbc.M103554200
    [4] Gubbels JA, Felder M, Horibata S, et al. MUC16 provides immune protection by inhibiting synapse formation between NK and ovarian tumor cells[J]. Mol Cancer, 2010, 9:11. doi:  10.1186/1476-4598-9-11
    [5] Belisle JA, Gubbels JA, Raphael CA, et al. Peritoneal natural killer cells from epithelial ovarian cancer patients show an altered phenotype and bind to the tumour marker MUC16(CA125)[J]. Immunology, 2007, 122:418-429. doi:  10.1111/j.1365-2567.2007.02660.x
    [6] Patankar MS, Jing Y, Morrison JC, et al. Potent suppression of natural killer cell response mediated by the ovarian tumor marker CA125[J]. Gynecol Oncol, 2005, 99:704-713. doi:  10.1016/j.ygyno.2005.07.030
    [7] Lanier LL. Natural killer cell receptor signaling[J]. Curr Opin Immunol, 2003, 15:308-314. doi:  10.1016/S0952-7915(03)00039-6
    [8] Tyler C, Kapur A, Felder M, et al. The mucin MUC16(CA125) binds to NK cells and monocytes from peripheral blood of women with healthy pregnancy and preeclampsia[J]. Am J Reprod Immunol, 2012, 68:28-37. doi:  10.1111/j.1600-0897.2012.01113.x
    [9] Radka S, Weston BS, Kieran W, et al. Exploring the glycosylation of serum CA125[J]. Int J Mol Sci, 2013, 14:15636-15654. doi:  10.3390/ijms140815636
    [10] 朱兰, 郎景和.肿瘤标记物在妇科领域中的应用[J].中国实验诊断学, 2012, 6:301-302. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgsyzdx200205029
    [11] Gipson IK, Blalock T, Tisdale A, et al. MUC16 is lost from the uterodome (pinopode) surface of the receptive human endometrium:in vitro evidence that MUC16 is a barrier to trophoblast adherence[J]. Biol Reprod, 2007, 78:134-142.
    [12] Pfisterer J, Mahner S. Towards individualised treatment in ovarian cancer[J]. Lancet Oncol, 2013, 14:101-102. doi:  10.1016/S1470-2045(13)70006-8
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(1)  / Tables(3)

    Article Metrics

    Article views (104) PDF downloads(4) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return