Volume 5 Issue 3
Jul.  2014
Turn off MathJax
Article Contents
Wei LI, Chun-mei HUANG, Wei WU, Ying-ying HU, Qian CHEN, ye GUO, Yu-juan ZHANG, Wei CUI. Short-term Biological Variation of Glycated Hemoglobin-A1c[J]. Medical Journal of Peking Union Medical College Hospital, 2014, 5(3): 270-272. doi: 10.3969/j.issn.1674-9081.2014.03.005
Citation: Wei LI, Chun-mei HUANG, Wei WU, Ying-ying HU, Qian CHEN, ye GUO, Yu-juan ZHANG, Wei CUI. Short-term Biological Variation of Glycated Hemoglobin-A1c[J]. Medical Journal of Peking Union Medical College Hospital, 2014, 5(3): 270-272. doi: 10.3969/j.issn.1674-9081.2014.03.005

Short-term Biological Variation of Glycated Hemoglobin-A1c

doi: 10.3969/j.issn.1674-9081.2014.03.005
  • Received Date: 2014-04-30
  • Publish Date: 2014-07-30
  •   Objective  To study the short-term biological variation of glycated hemoglobin-A1c (HbA1c).  Methods  Blood samples from 30 healthy volunteers [17 men and 13 women; aged 23-34 years (median, 24 years)] were drawn at 8:00 am, 12:00 pm, and 4:00 pm each day for 3 days (days 1, 3 and 5), and HbA1c values were measured in duplicate by high performance liquid chromatography (Variant Ⅱ Yurbo Analyser, Bio-Rad).  Results  The within-subject coefficient of variations (CVI) of total, male, and female subjects in this study were 1.39%, 1.49%, and 1.32%, respectively. The between-subject coefficient of variations (CVG) of the three groups mentioned above were 2.65%, 2.33%, and 2.90%, respectively. The analytical coefficient of variations (CVA) of these three groups were all 0.6%. The index of individuality (II) of the three groups were 0.5, 0.6, and 0.5, respectively. The 95% probabilities of reference change value (RCV) of the three groups were 4.22%, 4.45%, and 4.02%, respectively. The 99% probabilities of RCV were 5.19%, 5.86%, and 5.29%, respectively. Furthermore, intra- and inter-day biological variations of HbA1c were fairly constant among the population examined(P=0.28, 0.31), and no significant difference was observed between the male and female participants(P=0.18).  Conclusions  The short-term CVI and CVG of HbA1c in healthy individuals are minimal. The laboratory findings are quite constant and homogeneous for HbA1c in short term and between genders.
  • loading
  • [1] Fraser CG, Harris EK. Generation and application of data on biological variation in clinical chemistry[J]. Crit Rev Clin Lab Sci, 1989, 27:409-437. http://ndt.oxfordjournals.org/external-ref?access_num=10.3109/10408368909106595&link_type=DOI
    [2] Harris KH. Effects of intra-and interindividual variation on the appropriate use of normal ranges[J]. Clin Chem, 1974, 20:1535-1542. http://www.bioone.org/servlet/linkout?suffix=i1042-7260-38-2-217-b9&dbid=8&doi=10.1638%2F1042-7260(2007)038[0217%3ASBVOCC]2.0.CO%3B2&key=4430131
    [3] Ucar F, Erden G, Ginis Z, et al. Estimation of biological variation and reference change value of glycated hemoglobin (HbA1c) when two analytical methods are used[J]. Clin Biochem, 2013, 46:1548-1553. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=5cbe70ede524ea45d8213d511157d274
    [4] Kilpatrick ES, Maylor PW, Keevil BG. Biological variation of glycated haemoglobin.Implications for diabetes screening and monitoring[J]. Diabetes Care, 1998, 21:261-264. http://jcp.bmj.com/lookup/ijlink?linkType=ABST&journalCode=diacare&resid=21/2/261&atom=%2Fjclinpath%2F57%2F4%2F344.atom
    [5] Rohlfing C, Wiedmeyer HM, Little R, et al. Biological variation of glycohemoglobin[J]. Clin Chem, 2002, 48:1116-1118. http://europepmc.org/abstract/MED/12089188
    [6] Fraser CG. Biological variation:from principles to practice[M]. Washington DC:AACC Press, 2001.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (220) PDF downloads(11) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return