Protective Effect of Hypertonic Saline Solution on Lung Injury Induced by Intestinal Ischemia-reperfusion in Rabbits

  Objective  To study the effect of hypertonic saline solution (HS) on lung function in intestinal ischemia-reperfusion (ⅡR) models.

  Methods  Totally 72 rabbits were randomly divided into 4 groups (n=18):control group, ⅡR group, 4%HS group, and 7.5%HS group. The ⅡR models were produced by clamping super mesenteric artery (SMA) for 1 hour and declamping SMA for 6 hours in ⅡR group, 4%HS group, and 7.5%HS group, whereas animals in control group underwent sham operation. The corresponding sodium chloride solution was infused in the 2 treatment groups in 5 minutes before declamping SMA. Before and 2, 4, 6 hours after ⅡR, the plasma levels of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were measured. At 6 hours after ⅡR, 8 rabbits in each group were killed to measure the levels of lung water and myeloperoxidase (MPO) activity in lung tissue. Meanwhile, lung morphological changes were observed under light microscope.

  Results  The plasma TNF-α and IL-10 levels, the levels of lung water and MPO activity in lung tissue, and lung morphological score significantly increased in ⅡR group when compared with the control group(P < 0.05). Compared with the ⅡR group, the TNF-α levels in plasma, the levels of lung water and MPO activity in lung tissue, and lung morphological score in both two HS groups significantly decreased (P < 0.05), particularly in 7.5%HS group which was significantly lower than those in the 4%HS group (P < 0.05).

  Conclusions  HS has a protective effect on the lung function during ⅡR. Such a protective effect increases along with the increase of HS concentration within certain range.