Comparison of Two Different Scaffolds in Terms of Their in vitro Compatibilities with Chondrogenic-like Cells

  Objective  To study the compatibility of two different scaffolds with chondrogenic-like cells in vitro.

  Methods  Chondrogenic-like cells were derived from bone marrow mesenchymal stem cells. Chondrogenic-like cells and two different scaffolds(PGA scaffold and PGA\collagen composite scaffold) were cocultured in vitro. Morphological features were observed by HE staining and electron microscopy. Cell adhesion and proliferation were detected with MTT assay and compared to the control group.

  Results  Histological and ultrastructural observations showed the cells grew well on the surface of the PGA\collagen composite scaffold. The cells were not adhered on the surface of the PGA scaffold. The MTT assay showed that both two scaffolds did not inhibit the proliferation of chondrogenic-like cells.

  Conclusion  The PGA\collagen composite scaffold has good biocompatibility with chondrogenic-like cells and can be used as a vehicle for trachea tissue engineering.