Volume 2 Issue 1
Jan.  2011
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Xin-jie BAO, Jun-ji WEI, Ming FENG, Shan LU, Chun-hua ZHAO, Ren-zhi WANG. Effect of Transplantation of Bone Marrow-derived Mesenchymal Stem Cells on the Apoptosis of Neurons in Ischemic Rats[J]. Medical Journal of Peking Union Medical College Hospital, 2011, 2(1): 19-23. doi: 10.3969/j.issn.1674-9081.2011.01.004
Citation: Xin-jie BAO, Jun-ji WEI, Ming FENG, Shan LU, Chun-hua ZHAO, Ren-zhi WANG. Effect of Transplantation of Bone Marrow-derived Mesenchymal Stem Cells on the Apoptosis of Neurons in Ischemic Rats[J]. Medical Journal of Peking Union Medical College Hospital, 2011, 2(1): 19-23. doi: 10.3969/j.issn.1674-9081.2011.01.004

Effect of Transplantation of Bone Marrow-derived Mesenchymal Stem Cells on the Apoptosis of Neurons in Ischemic Rats

doi: 10.3969/j.issn.1674-9081.2011.01.004
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  • Corresponding author: WANG Ren-zhi Tel:010-65296071, E-mail:wangrz@126.com
  • Received Date: 2010-10-12
  • Publish Date: 2011-01-30
  •   Objective  To investigate the apoptosis of neurons in the penumbra of cerebral ischemic rats transplanted with bone marrow derived-mesenchymal stem cells(BMSCs).  Methods  Bone marrow of a healthy adult volunteer was collected, and BMSCs were cultivated and harvested.Adult rats subjected to 2 h middle cerebral artery occlusion(MCAO)were randomly assigned to the BMSCs-treated group(n=12)and the control group(n=12), in which rats were transplanted with 5×105 BMSCs or saline, respectively, into the ipsilateral brain parenchyma on the 3rd post-MCAO day.The modified neurological severity scores(mNSS)was used to measure functional recovery.On the 14th post-MCAO day, apoptotic cells in the penumbra were detected by TUNEL analysis.On the 28th post-MCAO day, neurons were stained by immunoflu-orescence, and hematoxylin-eosin staining was used to evaluate the relative ischemic volume.  Results  TUNEL-positive cells were significantly reduced(P=0.017), and in contrast, neuronal nuclei antigen(NeuN)-positive cells were significantly increased(P=0.015)in the penumbra in BMSCs-treated group compared with control group.The relative ischemic volume was decreased in the BMSCs-treated group(28.3±4.5)% compared with the control group(36.8±5.6)%(P=0.041).The mNSS was significantly decreased in BMSCs-treated group at each time point starting on the 14th post-MCAO day compared with control group(P < 0.05).  Conclusion  BMSCs treatment can decrease ischemic volume and improve functional recovery after cerebral ischemia, which may, at least partially, attribute to the reduced apoptosis of neurons in the penumbra.
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