Volume 14 Issue 5
Sep.  2023
Turn off MathJax
Article Contents
WANG Zhe, ZHAO Ying, XU Yingchun. Advances in Laboratory Diagnosis of Bacterial Vaginosis[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(5): 1053-1059. doi: 10.12290/xhyxzz.2022-0677
Citation: WANG Zhe, ZHAO Ying, XU Yingchun. Advances in Laboratory Diagnosis of Bacterial Vaginosis[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(5): 1053-1059. doi: 10.12290/xhyxzz.2022-0677

Advances in Laboratory Diagnosis of Bacterial Vaginosis

doi: 10.12290/xhyxzz.2022-0677
More Information
  • Corresponding author: ZHAO Ying, E-mail: zhaoying28062806@163.com
  • Received Date: 2022-11-28
  • Accepted Date: 2023-01-13
  • Publish Date: 2023-09-30
  • Bacterial vaginosis (BV), an imbalance of normal vaginal microecology, is manifested as a mixed infection. It is characterized by a decrease of normal hydrogen peroxide-producing lactobacillus and an overgrowth of anaerobic bacteria (mobiluncus, prevotella) and facultative bacteria (gardnerella vaginalis, atopo vaginalis). BV is a risk factor for pelvic inflammatory disease, infertility, sexually transmitted pathogens (human papillomavirus, human immunodeficiency virus, neisseria gonorrhoeae, chlamydia trachomatis, etc.) infection. In addition, BV during pregnancy can lead to adverse obstetrical outcomes, including early miscarriage, premature delivery, postpartum endometritis, and low birth weight. The pathogens causing BV are diverse and have a high recurrence rate after treatment. About 10% to 50% of BV patients have no clinical symptoms and are easy to miss diagnosis. Rapid and accurate laboratory detection can provide timely diagnosis and treatment for BV patients and avoid adverse outcomes. Therefore, this paper reviews the research progress of diagnostic criteria, vaginal microecological evaluation and novel detection methods for BV, in order to provide reference for clinical diagnosis and treatment of BV.
  • loading
  • [1] Marrazzo JM, Martin DH, Watts DH, et al. Bacterial vaginosis: Identifying research gaps proceedings of a workshop sponsored by DHHS/NIH/NIAID[J]. Sex Transm Dis, 2010, 37: 732-744. doi:  10.1097/OLQ.0b013e3181fbbc95
    [2] Ravel J, Moreno I, Simón C. Bacterial vaginosis and its association with infertility, endometritis, and pelvic inflammatory disease[J]. Am J Obstet Gynecol, 2021, 224: 251-257. doi:  10.1016/j.ajog.2020.10.019
    [3] Force UPST, Owens DK, Davidson KW, et al. Screening for bacterial vaginosis in pregnant persons to prevent preterm delivery: US Preventive Services Task Force Recommendation Statement[J]. JAMA, 2020, 323: 1286-1292. doi:  10.1001/jama.2020.2684
    [4] 中华医学会妇产科学分会感染性疾病协作组. 细菌性阴道病诊治指南(2021修订版)[J]. 中华妇产科杂志, 2021, 56: 3-6.
    [5] Coudray MS, Madhivanan P. Bacterial vaginosis-A brief synopsis of the literature[J]. Eur J Obstet Gynecol Reprod Biol, 2020, 245: 143-148. doi:  10.1016/j.ejogrb.2019.12.035
    [6] Amsel R, Totten PA, Spiegel CA, et al. Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations[J]. Am J Med, 1983, 74: 14-22. doi:  10.1016/0002-9343(83)91112-9
    [7] Workowski KA, Bachmann LH, Chan PA, et al. Sexually Transmitted Infections Treatment Guidelines, 2021[J]. MMWR Recomm Rep, 2021, 70: 1-187.
    [8] American College of Obstetricians and Gynecologists. Vaginitis in nonpregnant patients. ACOG Practice Bulletin No. 215[J]. Obstet Gynecol, 2020, 135: 243-245. doi:  10.1097/AOG.0000000000003605
    [9] Coleman JS, Gaydos CA. Molecular Diagnosis of Bacterial Vaginosis: an Update[J]. J Clin Microbiol, 2018, 56: e00342-18.
    [10] Nugent RP, Krohn MA, Hillier SL. Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation[J]. J Clin Microbiol, 1991, 29: 297-301. doi:  10.1128/jcm.29.2.297-301.1991
    [11] Mitchell CM, Srinivasan S, Ma N, et al. Bacterial Communities Associated With Abnormal Nugent Score in Postmenopausal Versus Premenopausal Women[J]. J Infect Dis, 2021, 223: 2048-2052. doi:  10.1093/infdis/jiaa675
    [12] 王辰, 王慧慧, 李焕荣, 等. 《2018欧洲国际性病控制联盟/世界卫生组织关于阴道分泌物(阴道炎症)管理指南》解读[J]. 中国实用妇科与产科杂志, 2018, 34: 1360-1365. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGSF201812016.htm
    [13] Jung H, Ehlers MM, Peters RPH, et al. Growth Forms of Gardnerella spp. and Lactobacillus spp. on Vaginal Cells[J]. Front Cell Infect Microbiol, 2020, 10: 71. doi:  10.3389/fcimb.2020.00071
    [14] 中华医学会妇产科学分会感染性疾病协作组. 阴道微生态评价的临床应用专家共识[J]. 中华妇产科杂志, 2016, 51: 721-723. doi:  10.3760/cma.j.issn.0529-567x.2016.10.001
    [15] 杨文方, 李旭, 王翔, 等. 阴道微生态的临床研究及意义[J]. 实用妇产科杂志, 2012, 28: 205-209. https://www.cnki.com.cn/Article/CJFDTOTAL-SFCZ201203021.htm
    [16] 王敏, 宋磊, 姚立红, 等. 223例健康育龄妇女阴道内乳酸杆菌菌群的鉴定[J]. 中国妇产科临床杂志, 2009: 200-203. https://www.cnki.com.cn/Article/CJFDTOTAL-FKLC200903016.htm
    [17] Ng S, Chen M, Kundu S, et al. Large-scale characterisation of the pregnancy vaginal microbiome and sialidase activity in a low-risk Chinese population[J]. NPJ Biofilms Microbiomes, 2021, 7: 89. doi:  10.1038/s41522-021-00261-0
    [18] Word Health Organization. Laboratory diagnosis of sexually transmitted infections, including human immunodeficiency virus[EB/OL ]. (2013-01-01)[2023-08-01]. https://apps.who.int/iris/bitstream/handle/10665/85343/9789241505840_eng.pdf.
    [19] Liu GJ, Wang B, Zhang Y, et al. A tetravalent sialic acid-coated tetraphenylethene luminogen with aggregation-induced emission characteristics: design, synthesis and application for sialidase activity assay, high-throughput screening of sialidase inhibitors and diagnosis of bacterial vaginosis[J]. Chem Commun (Camb), 2018, 54: 10691-10694. doi:  10.1039/C8CC06300A
    [20] Wu S, Lin X, Hui KM, et al. A Biochemiluminescent Sialidase Assay for Diagnosis of Bacterial Vaginosis[J]. Sci Rep, 2019, 9: 20024. doi:  10.1038/s41598-019-56371-5
    [21] 袁丽莉, 袁丽艳. 阴道分泌物BV3项及常规检测结果分析[J]. 检验医学与临床, 2011, 8: 1372-1373. https://www.cnki.com.cn/Article/CJFDTOTAL-JYYL201111059.htm
    [22] 经先振, 王齐, 牛国平. 阴道分泌物干化学酶法与传统方法检测结果的比较[J]. 医学理论与实践, 2015, 28: 3267-3268, 3283. https://www.cnki.com.cn/Article/CJFDTOTAL-YXLL201523065.htm
    [23] 何亮, 尹洪莉, 林维佳, 等. 三种检测方法对细菌性阴道病诊断价值的比较[J]. 昆明医科大学学报, 2020, 41: 72-76. https://www.cnki.com.cn/Article/CJFDTOTAL-KMYX202008014.htm
    [24] 吴秀珍, 马菲菲, 林丽华, 等. 阴道微生态评价系统检测阴道分泌物诊断细菌性阴道病的价值[J]. 医疗装备, 2021, 34: 32-33. https://www.cnki.com.cn/Article/CJFDTOTAL-YLZB202120015.htm
    [25] 蔡斌, 马洁, 吴晓梅, 等. 阴道微生态评价在妇科门诊中的应用价值[J]. 中国微生态学杂志, 2022, 34: 711-713. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGWS202206016.htm
    [26] 魏冰, 陆庭嫣, 沈俐, 等. BD Affirm VP芯片检测技术在阴道分泌物常规检测中的应用[J]. 检验医学, 2017, 32: 1134-1136. https://www.cnki.com.cn/Article/CJFDTOTAL-SHYY201712016.htm
    [27] Thompson A, Timm K, Borders N, et al. Diagnostic performance of two molecular assays for the detection of vaginitis in symptomatic women[J]. Eur J Clin Microbiol Infect Dis, 2020, 39: 39-44.
    [28] Deng T, Shang A, Zheng Y, et al. Log (Lactobacillus crispatus/ Gardnerella vaginalis): a new indicator of diagnosing bacterial vaginosis[J]. Bioengineered, 2022, 13: 2981-2991.
    [29] Niu XX, Sun RH, Liu ZH, et al. Length-Heterogeneity Polymerase Chain Reaction as a Diagnostic Tool for Bacterial Vaginosis[J]. Chin Med J (Engl), 2018, 131: 2242-2243.
    [30] Lai L, Wu Y, Li Y, et al. A Capillary Electrophoresis Method for Analyzing the Diversity of Vaginal Microbiome[J]. Clin Lab, 2022, 68: 523-531.
    [31] Ruffier d'Epenoux L, Tessier E, Guillouzouic A, et al. Assessment of the Performance of the Aptima Bacterial Vaginosis Assay Over a 3-Month Period in a French Hospital[J]. Microbiol Spectr, 2022, 10: e0130122.
    [32] 吕治, 彭国丽, 王克迪, 等. 荧光原位杂交技术诊断细菌性阴道病的方法学研究[J]. 临床和实验医学杂志, 2015: 1139-1142. https://www.cnki.com.cn/Article/CJFDTOTAL-SYLC201514003.htm
    [33] Sousa LGV, Castro J, França A, et al. A New PNA-FISH Probe Targeting Fannyhessea vaginae[J]. Front Cell Infect Microbiol, 2021, 11: 779376.
    [34] Bostwick GD, Hunt CA. Utility of Next-generation sequencing in Managing Bacterial Vaginosis: Examples from Clinical Practice[EB/OL]. (2016-08-10)[2023-08-01]. https://www.longdom.org/open-access/utility-of-nextgener-ation-sequencing-in-manag-ing-bacterial-vaginosis-examples-from-clinical-practice-2167-0420-1000322.pdf.
    [35] 戴媛媛, 马筱玲. 宏基因组二代测序技术在临床病原学诊断中的应用[J]. 临床检验杂志, 2021, 39: 1-5. https://www.cnki.com.cn/Article/CJFDTOTAL-LCJY202101002.htm
    [36] 《中华传染病杂志》编辑委员会. 中国宏基因组学第二代测序技术检测感染病原体的临床应用专家共识[J]. 中华传染病杂志, 2020, 38: 681-689. https://www.cnki.com.cn/Article/CJFDTOTAL-HBYX202107002.htm
    [37] Rodríguez-Nava C, Cortés-Sarabia K, Avila-Huerta MD, et al. Nanophotonic Sialidase Immunoassay for Bacterial Vaginosis Diagnosis[J]. ACS Pharmacol Transl Sci, 2021, 4: 365-371.
    [38] Srinivasan S, Munch MM, Sizova MV, et al. More Easily Cultivated Than Identified: Classical Isolation With Molecular Identification of Vaginal Bacteria[J]. J Infect Dis, 2016, 214 Suppl 1: S21-S28.
    [39] Liu Z, Bian L, Yeoman CJ, et al. Bacterial Vaginosis Monitoring with Carbon Nanotube Field-Effect Transistors[J]. Anal Chem, 2022, 94: 3849-3857.
    [40] Song Y, He L, Zhou F, et al. Segmentation, Splitting, and Classification of Overlapping Bacteria in Microscope Images for Automatic Bacterial Vaginosis Diagnosis[J]. IEEE J Biomed Health Inform, 2017, 21: 1095-1104.
    [41] Wang Z, Zhang L, Zhao M, et al. Deep Neural Networks Offer Morphologic Classification and Diagnosis of Bacterial Vaginosis[J]. J Clin Microbiol, 2021, 59: e02236-20.
    [42] Hao R, Liu L, Zhang J, et al. A Data-Efficient Framework for the Identification of Vaginitis Based on Deep Learning[J]. J Healthc Eng, 2022, 2022: 1929371.
    [43] 王瑶, 孙宏莉, 赵颖, 等. 自动化镜检结合人工智能分析系统对阴道分泌物革兰氏染色涂片形态学的准确性评价[J]. 协和医学杂志, 2021, 12: 503-509. doi:  10.12290/xhyxzz.2021-0412
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Tables(1)

    Article Metrics

    Article views (238) PDF downloads(118) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return