Abstract: Anti-Müllerian hormone(AMH) is a dimeric glycoprotein secreted by follicular granulosa cell and sertoli cell. AMH inhibits the recruitment and growth of follicles in female, and adjusts the function of leydig cells in male. Serum AMH level is a potential marker for ovarian reserve. At present, the article elaborated its clinical application:the level of serum AMH can be used to evaluate ovarian reserve, to predict ovarian response during assisted reproductive process, to evaluate ovarian reserve of cancer patients, to diagnose polycystic ovary syndrome, premature ovarian failure and predictive menopause. There are few studies on the detection methods and reference intervals. With the development of detection technology, AMH has become more widely used in clinical medicine. The physiology, clinical application, assay methods and reference intervals of AMH are discussed in this article.