Abstract: The diagnosis and surgical treatment of insulinoma is dependent on accurate localization with imaging modalities. Glucagon-like peptide-1 receptor (GLP-1R) imaging that targets pancreatic β cells has been highlighted for detecting insulinomas in recent years. Since the high sensitivity and specificity (se. 99%, sp. 100%) of GLP-1R imaging in localizing insulinoma, GLP-1R positron emission tomography/computed tomography (PET/CT) with ⁶⁸Ga-exendin-4 is recommended to be introduced into clinical practice. This paper introduced the application of GLP-1R imaging in insulinoma, and took the GLP-1R PET/CT with ⁶⁸Ga-exendin-4 as an example to elaborate the operation standard of this technique, aiming to provide a reference for clinical practice.