Abstract: Infectious diseases are still the main diseases that threaten human health. Traditional diagnostic technology has been unable to meet the clinical needs. The development of pathogenic diagnosis technology is of vital importance to the diagnosis and treatment of infectious diseases. In recent years, non-cultured new diagnostic technologies have been rapidly developing, especially the real-time detection based on lateral flow immunoassay and real-time polymerase chain reaction. The continuous application of proteomics mass-spectrometry and high-throughput sequencing technology in clinical practice makes the clinical diagnosis of infectious diseases more convenient, faster and more accurate. However, it is necessary to correctly handle the problems in the clinical application of new diagnostic technology. Diagnostic tests should be assessed from the angle of evidence-based medicine and the applied crowds to gather the best evidence, so that they can be applied to the appropriate people and achieve the best effect, making the application of new technology in etiologic diagnosis really solve the problem of clinical diagnosis and eventually provide the basis for scientific decision-making about the clinical diagnosis and treatment of infectious diseases.