Abstract:
Objective To investigate the pathogen distribution of positive blood culture in Peking Union Medical College Hospital from January 2008 to December 2010.
Methods BacT/Alert 3D 480 and BD Bactec 9120 automatic blood culture instruments were used for blood culture. Microorganism identification methods including Vitek 2 compact and Pheonix 100 microbial identification system, API series kit, and Chromagar chromogenic medium were used to identify the pathogens.
Results The number of positive blood culture from outpatients and inpatients of Peking Union Medical College Hospital in 2008, 2009, and 2010 was 928, 942, and 913, respectively. The most common bacteria was coagulase-negative Staphylococci (27.0%, 27.7%, 26.0%), the second was Escherichia coli (13.3%, 14.4%, 17.1%), followed by Staphylococcus aureus (6.8%, 6.3%, 5.0%), Klebsiella pneumonia (4.8%, 4.1%, 6.1%), Acinetobacter baumannii (4.7%, 5.8%, 5.6%), Pseudomonas aeruginosa (3.0%, 4.0%, 3.4%), Enterococcus faecium (5.0%, 4.8%, 3.0%), and Enterococcus faecalis (2.5%, 3.4%, 3.0%). The most common fungi from blood culture in the three years were Candida albican (1.0%, 1.6%, 2.1%) and Candida parapsilosis (1.1%, 0.7%, 1.3%).
Conclusion We should pay more attention to the isolation and identification of fastidious bacteria, less common bacteria, and fungi.