Expression and Significance of GATA-3、 H3K27me3 in Tibet with Bladder Urothelial Carcinoma
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摘要: 目的 探讨西藏地区膀胱尿路上皮癌(bladder urothelialcarcinoma, BUC)患者 GATA-3 和 H3K27me3 的表达及其临床意义。 方法 回顾性收集西藏自治区人民医院 2016 年 1 月-2021 年 12 月间行膀胱癌手术的 BUC 患者的临床资料及其病理组织,以经尿道膀胱黏膜活检术获取的正常膀胱组织作为对照,采用免疫组化染色方法检测 BUC 和正常膀胱组织中 GATA-3 和 H3K27me3 的表达水平,同时结合临床病理特征分析 GATA-3 和 H3K27me3 在藏族 BUC 患者中的表达差异。 结果 共入选 BUC患者 70 例, 其中男性 51 例,女性 19 例,平均年龄(60.5±12.0)岁,同时收集同期正常膀胱组织标本 20 份,均来自于藏族患者。免疫组化检测结果显示, GATA-3 在 BUC及正常膀胱组织中高表达率分别为 70%(49/70)和 100%(20/20), GATA-3 高表达与男性、病理分级低、组织非浸润相关(P 均<0.05); H3K27me3 在 BUC 及正常膀胱组织中高表达率分别为 45.7%(32/70)和 20%(4/20), H3K27me3 高表达仅与男性相关(P<0.05)。 结论 GATA-3 在西藏地区 BUC 患者膀胱组织中表达下调,且随着肿瘤级别的升高表达显著下调,提示 GATA-3 可能参与 BUC 发生、发展并与其恶性程度相关,为临床诊疗及判断疾病预后提供了参考。H3K27me3 在西藏地区 BUC 患者膀胱组织中表达上调,提示 H3K27me3 有望成为诊断膀胱尿路上皮癌的新型免疫标志物。Abstract: Objective To investigate the expression and clinical significance of GATA-3 and H3K27me3 in Tibet with bladder urothelial carcinoma (BUC). Methods BUC and normal bladder tissues were collected retrospectively from January 2016 to December 2021 in the People's Hospital of Tibet Autonomous Region. The expression of GATA-3 and H3K27me3 in both tissues was detected by immunohistochemical method, and the clinical and pathological characteristics were statistically analyzed. Results A total of 70 patients with BUC were selected, including 51 males and 19 females, with an average age of (60.5± 12.0) years. At the same time, 20 normal bladder tissue samples were collected during the same period. All cases were Tibetan patients. Immunohistochemistry results showed that the high expression rate of GATA-3 in BUC and normal bladder tissue was 70% (49/70) and 100% (20/20), respectively. High expression of GATA-3 was associated with male, low pathological grade, and non invasive tissue (P<0.05). The high expression rate of H3K27me3 in BUC and normal bladder tissue was 45.7% (32/70) and 20% (4/20), respectively. High expression of H3K27me3 was only associated with male (P<0.05). Conclusions The expression of GATA-3 is down regulated in patients with BUC in Tibet, and significantly down regulated with the increase of tumor grade, suggesting that GATA-3 may be involved in the occurrence and development of BUC and related to its malignancy, providing a reference for clinical diagnosis and treatment and judging disease prognosis. The expression of H3K27me3 in BUC in Tibet is higher than that in normal bladder tissue, suggesting that H3K27me3 may be a new immune marker for diagnosis of bladder urothelial carcinoma.
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Key words:
- Tibet /
- Bladder urothelial carcinoma /
- Clinicopathological characteristics /
- GATA-3 /
- H3K27me3
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