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SLAMF7在小鼠肠道组织及肠道炎症中的表达及意义

杨燕,  陆雪珂,  葛秀珍,  娄运伟,  常廷民

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杨燕, 陆雪珂, 葛秀珍, 娄运伟, 常廷民. SLAMF7在小鼠肠道组织及肠道炎症中的表达及意义[J]. 协和医学杂志. doi: 10.12290/xhyxzz.2023-0540
引用本文: 杨燕, 陆雪珂, 葛秀珍, 娄运伟, 常廷民. SLAMF7在小鼠肠道组织及肠道炎症中的表达及意义[J]. 协和医学杂志. doi: 10.12290/xhyxzz.2023-0540
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YANG Yan, LU Xueke, GE Xiuzhen, LOU Yunwei, CHANG Tingmin. Expression and Significance of SLAMF7 in Intestinal Tissue and Intestinal Inflammation in Mice[J]. Medical Journal of Peking Union Medical College Hospital. doi: 10.12290/xhyxzz.2023-0540
Citation: YANG Yan, LU Xueke, GE Xiuzhen, LOU Yunwei, CHANG Tingmin. Expression and Significance of SLAMF7 in Intestinal Tissue and Intestinal Inflammation in Mice[J]. Medical Journal of Peking Union Medical College Hospital. doi: 10.12290/xhyxzz.2023-0540
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SLAMF7在小鼠肠道组织及肠道炎症中的表达及意义

doi: 10.12290/xhyxzz.2023-0540

  • 1 新乡医学院第一附属医院消化内科, 河南新乡 453100;

  • 2 新乡医学院医学技术学院, 河南新乡 453003

基金项目: 

国家自然科学基金（81971491）；河南省高校科技创新人才支持计划（23HASTIT049）

详细信息
    通讯作者:

    娄运伟,E-mail:yloul@xxmu.edu.cn

    常廷民,E-mail:ctminmail@163.com

  • 中图分类号: R392

Expression and Significance of SLAMF7 in Intestinal Tissue and Intestinal Inflammation in Mice

  • 1 Department of Gastroenterology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, China;

  • 2 School of Medical Technology, Xinxiang Medical University, Xinxiang, Henan 453003, China

Funds: 

National Natural Science Foundation of China (81971491)

    摘要
  • 摘要: 目的 探讨细胞表面受体信号淋巴细胞活化分子受体7（signaling lymphocytic activation molecule receptor family 7，SLAMF7）在小鼠正常肠道组织和肠道炎症组织中的表达及意义。 方法 选择8~10周龄C57BL/6J野生型（wild-type，WT）雄性小鼠5只，正常饮用水喂养，提取其结肠固有层淋巴细胞（lamina propria lymphocytes，LPLs）和肠上皮细胞（intestinal epithelial cells，IECs），Trizol试剂提取细胞总RNA，逆转录聚合酶链式反应（reverse transcription polymerase chain reaction，RT-PCR）和实时荧光定量聚合酶链式反应（real-time fluorescence quantitative polymerase chain reaction，RT-qPCR）检测细胞中SLAMF7的mRNA表达情况。选择8~10周龄C57BL/6J野生型（wild-type，WT）雄性小鼠10只，随机分为对照组和模型组，每组各5只。对照组用正常饮用水喂养5 d；模型组用含2.5%葡聚糖硫酸酯钠盐（dextran sodium sulfate，DSS）的饮用水喂养5 d，建立小鼠溃疡性结肠炎（ulcerative colitis，UC）模型。第5天处死两组小鼠，采用流式细胞术检测对照组与模型组SLAMF7在免疫细胞亚群中的表达情况。 结果 相较于结肠IECs，SLAMF7在结肠LPLs中表达较高（P=0.017）； DSS诱导肠炎后，SLAMF7在中性粒细胞中的表达上调（P=0.001），在CD4+T细胞、CD8+T细胞、B细胞、巨噬细胞、经典树突状细胞（conventional dendritic cell，cDC）中的表达无明显变化（P均>0.05）。 结论 SLAMF7可能通过中性粒细胞相关途径在UC的发生发展过程中发挥重要作用。
    Abstract: Objective To investigate the expression and significance of cell surface receptor signaling lymphocyte activation molecule receptor 7 (SLAMF7) in normal intestinal tissues and intestinal inflammatory tissues of mice. Methods Five C57BL/6J wild-type male mice aged 8-10 weeks were taken and fed normally. Lamina propria lymphocytes (LPLs) and intestinal epithelial cells (IECs) were extracted, and total cell RNA was extracted by Trizol reagent. The mRNA expression of SLAMF7 in cells was detected by reverse transcription polymerase chain reaction (RT-PCR) and real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). Ten C57BL/6J wild-type male mice aged 8-10 weeks were randomly divided into control group (n=5) and model group (n=5). The control group was fed with normal drinking water for 5 days, the model group was fed with 2.5% dextran sodium sulfate (DSS) drinking water for 5 days to establish a model of ulcerative colitis(UC), and the mice in both groups were killed on the 5th day. Flow cytometry was used to detect the expression of SLAMF7 in immune cell subpopulations in the control group and the model group. Results Compared with colonic IECs, the expression of SLAMF7 was higher in colonic LPLs (P=0.017). After DSS induced enteritis, the expression of SLAMF7 was up-regulated in neutrophils (P=0.001), but had no significant changes in CD4+T cells, CD8+T cells, B cells, macrophages and conventional dendritic cell (all P＞0.05). Conclusion SLAMF7 may play a certain role in the occurrence and development of UC through neutrophilrelated pathways.
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  • 网络出版日期:  2024-02-06

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