宏基因组高通量测序技术的临床应用：现状、挑战与前景

刁振丽; 李金明

doi:10.12290/xhyxzz.2023-0089

宏基因组高通量测序技术的临床应用：现状、挑战与前景

doi: 10.12290/xhyxzz.2023-0089

刁振丽^{1, 2},
李金明^{1, 2, ,}

1.
北京医院国家老年医学中心国家卫生健康委临床检验中心中国医学科学院老年医学研究院，北京 100730
2.
国家卫生健康委临床检验中心/北京协和医学院/中国医学科学院，北京 100730

详细信息

通讯作者:
李金明, E-mail: jmli@nccl.org.cn

中图分类号: R446.5; R51
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- 被引次数: 0
出版历程
- 收稿日期: 2023-02-17
- 录用日期: 2023-03-20
- 网络出版日期: 2023-03-29
- 刊出日期: 2023-09-30

Metagenomic Next-generation Sequencing: Current Status, Challenges and Prospects of Clinical Application

DIAO Zhenli^{1, 2},
LI Jinming^{1, 2
, ,}

1.
National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, Beijing 100730, China
2.
National Center for Clinical Laboratories, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China

More Information

Corresponding author: LI Jinming, E-mail: jmli@nccl.org.cn

摘要

摘要: 传统微生物学检测方法难以满足临床复杂多变的病原体检测需求。近年来，新兴的宏基因组高通量测序(metagenomic next-generation sequencing, mNGS)技术被广泛应用于各种感染性疾病诊断、新发及突发传染病病因分析、耐药基因检测和宿主免疫应答分析等领域。mNGS检测流程十分复杂，检测过程中每一步骤引入的变异均会影响检测结果的准确性。检测方法缺乏标准化、人员认知和能力不足、仪器试剂成本高昂是mNGS临床推广面临的主要障碍，因此亟需对检测方法进行标准化、规范化，快速提升检测人员的认知和综合能力。未来，整合mNGS病原体信息、转录组信息和耐药基因信息以提高对感染性疾病患者的临床管理水平是mNGS检测的重要发展方向。
- 宏基因组 /
- 高通量测序 /
- 感染 /
- 标准化
Abstract: Traditional microbial detection methods have difficulty meeting the needs of pathogen detection in the clinic. In recent years, metagenomic next-generation sequencing (mNGS) technology has emerged and been widely used in the diagnosis of various infectious diseases, the etiological analysis of emerging infectious diseases, the detection of drug resistance genes and the analysis of the host immune response. However, the mNGS workflow is complicated, and the variation introduced in each step will influence the accuracy of the detection results. In addition, the absence of standardized methods, the lack of personnel knowledge and ability, and the high cost of instruments and reagents are also creating main obstacles to the clinical application of mNGS. Therefore, standardization and normalization of methods as well as improvement of personnel knowledge and ability are urgently needed for mNGS detection. The integration of pathogen, transcriptome and antibiotic resistance genes information to comprehensively improve the clinical management of patients with infectious diseases is an important direction of mNGS detection.
- metagenomic /
- next-generation sequencing /
- infectious /
- standardized
注释:

作者贡献：刁振丽负责资料收集和论文撰写；李金明负责选题设计并审阅定稿。

利益冲突：所有作者均声明不存在利益冲突

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参考文献(34)

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宏基因组高通量测序技术的临床应用：现状、挑战与前景

doi: 10.12290/xhyxzz.2023-0089

通讯作者:
李金明, E-mail: jmli@nccl.org.cn

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Metagenomic Next-generation Sequencing: Current Status, Challenges and Prospects of Clinical Application

Corresponding author: LI Jinming, E-mail: jmli@nccl.org.cn

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宏基因组高通量测序技术的临床应用：现状、挑战与前景

doi: 10.12290/xhyxzz.2023-0089

通讯作者: 李金明, E-mail: jmli@nccl.org.cn

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出版历程

Metagenomic Next-generation Sequencing: Current Status, Challenges and Prospects of Clinical Application

Corresponding author: LI Jinming, E-mail: jmli@nccl.org.cn

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出版历程

目录

通讯作者:
李金明, E-mail: jmli@nccl.org.cn