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宏基因组高通量测序技术的临床应用:现状、挑战与前景

刁振丽 李金明

刁振丽, 李金明. 宏基因组高通量测序技术的临床应用:现状、挑战与前景[J]. 协和医学杂志, 2023, 14(5): 905-910. doi: 10.12290/xhyxzz.2023-0089
引用本文: 刁振丽, 李金明. 宏基因组高通量测序技术的临床应用:现状、挑战与前景[J]. 协和医学杂志, 2023, 14(5): 905-910. doi: 10.12290/xhyxzz.2023-0089
DIAO Zhenli, LI Jinming. Metagenomic Next-generation Sequencing: Current Status, Challenges and Prospects of Clinical Application[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(5): 905-910. doi: 10.12290/xhyxzz.2023-0089
Citation: DIAO Zhenli, LI Jinming. Metagenomic Next-generation Sequencing: Current Status, Challenges and Prospects of Clinical Application[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(5): 905-910. doi: 10.12290/xhyxzz.2023-0089

宏基因组高通量测序技术的临床应用:现状、挑战与前景

doi: 10.12290/xhyxzz.2023-0089
详细信息
    通讯作者:

    李金明, E-mail: jmli@nccl.org.cn

  • 中图分类号: R446.5; R51

Metagenomic Next-generation Sequencing: Current Status, Challenges and Prospects of Clinical Application

More Information
  • 摘要: 传统微生物学检测方法难以满足临床复杂多变的病原体检测需求。近年来,新兴的宏基因组高通量测序(metagenomic next-generation sequencing, mNGS)技术被广泛应用于各种感染性疾病诊断、新发及突发传染病病因分析、耐药基因检测和宿主免疫应答分析等领域。mNGS检测流程十分复杂,检测过程中每一步骤引入的变异均会影响检测结果的准确性。检测方法缺乏标准化、人员认知和能力不足、仪器试剂成本高昂是mNGS临床推广面临的主要障碍,因此亟需对检测方法进行标准化、规范化,快速提升检测人员的认知和综合能力。未来,整合mNGS病原体信息、转录组信息和耐药基因信息以提高对感染性疾病患者的临床管理水平是mNGS检测的重要发展方向。
    作者贡献:刁振丽负责资料收集和论文撰写;李金明负责选题设计并审阅定稿。
    利益冲突:所有作者均声明不存在利益冲突
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出版历程
  • 收稿日期:  2023-02-17
  • 录用日期:  2023-03-20
  • 网络出版日期:  2023-03-29
  • 刊出日期:  2023-09-30

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