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视神经脊髓炎谱系疾病相关神经病理性疼痛动物模型研究进展

宋淑佳 裴丽坚 徐雁 张雪 黄宇光

宋淑佳, 裴丽坚, 徐雁, 张雪, 黄宇光. 视神经脊髓炎谱系疾病相关神经病理性疼痛动物模型研究进展[J]. 协和医学杂志, 2023, 14(3): 598-602. doi: 10.12290/xhyxzz.2022-0647
引用本文: 宋淑佳, 裴丽坚, 徐雁, 张雪, 黄宇光. 视神经脊髓炎谱系疾病相关神经病理性疼痛动物模型研究进展[J]. 协和医学杂志, 2023, 14(3): 598-602. doi: 10.12290/xhyxzz.2022-0647
SONG Shujia, PEI Lijian, XU Yan, ZHANG Xue, HUANG Yuguang. Research Progress on the Animal Models of Neuromyelitis Optica Spectrum Disorders-related Neuropathic Pain[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(3): 598-602. doi: 10.12290/xhyxzz.2022-0647
Citation: SONG Shujia, PEI Lijian, XU Yan, ZHANG Xue, HUANG Yuguang. Research Progress on the Animal Models of Neuromyelitis Optica Spectrum Disorders-related Neuropathic Pain[J]. Medical Journal of Peking Union Medical College Hospital, 2023, 14(3): 598-602. doi: 10.12290/xhyxzz.2022-0647

视神经脊髓炎谱系疾病相关神经病理性疼痛动物模型研究进展

doi: 10.12290/xhyxzz.2022-0647
基金项目: 

中央高水平医院临床科研专项 2022-PUMCH-B-006

中央高校基本科研业务费专项基金 3332021015

详细信息
    通讯作者:

    黄宇光, E-mail: garypumch@163.com

  • 中图分类号: R741.02;R-332

Research Progress on the Animal Models of Neuromyelitis Optica Spectrum Disorders-related Neuropathic Pain

Funds: 

National High Level Hospital Clinical Research Funding 2022-PUMCH-B-006

Fundamental Research Funds for the Central Universities 3332021015

More Information
  • 摘要: 视神经脊髓炎谱系疾病(neuromyelitis optica spectrum disorders, NMOSD)是一种中枢神经系统炎性脱髓鞘性疾病,临床特征包括视神经炎、纵向长节段脊髓炎及其引起的疼痛,其中以神经病理性疼痛最为常见。目前已有研究证实神经病理性疼痛在NMOSD动物模型中存在,但受限于动物模型的缺乏,疼痛机制尚不明确,未来亟需建立可靠且简便易行的NMOSD神经病理性疼痛动物模型,以阐明发病机制,探索有效的预防及治疗策略。本文就NMOSD动物模型的建立及疼痛机制的研究进展作一综述,以期为建立理想的NMOSD神经病理性疼痛动物模型提供参考。
    作者贡献:宋淑佳负责查阅文献资料、撰写论文;裴丽坚、徐雁、张雪、黄宇光负责指导论文撰写、提出修改意见。
    利益冲突:所有作者均声明不存在利益冲突
  • 表  1  NMOSD动物模型及其特征

    文献 动物 模型 病变位置 AQP4缺失 GFAP缺失 炎性细胞浸润 补体沉积 脱髓鞘 AQP4-IgG剂量 注射后观察时间点 行为表现 疼痛表现
    Kinoshita等[19] 雌性Lewis大鼠 EAE+NMO-IgG腹腔注射 脊髓 巨噬细胞、中性粒细胞、嗜酸性粒细胞 20 mg/d×4 d 4 d EAE评分增加 -
    Chan等[18] 雌性C57BL/6小鼠 EAE+NMO-IgG腹腔注射 脊髓 2 mg/d×3 d 4 d EAE评分为0 -
    Saini等[20] 雌性C57/BL6小鼠 EAE+ NMO-IgG+补体腹腔注射 脊髓、视神经 T细胞、粒细胞 - 10 mg 0~60 d EAE评分增加 -
    Kurosawa等[16] 雌性Lewis大鼠 EAE+ AQP4单克隆抗体(E5415)腹腔注射 脊髓 中性粒细胞 1 mg 2 d EAE评分增加 机械痛阈值降低持续至建模后21 d
    Luo等[21] 雌性C57BL/6 WT小鼠 EAE+MELFUS+NMO-IgG+补体静脉注射 脊髓、大脑和视神经 单核细胞 100 μg 7 d - -
    Saadoun等[24] CD1小鼠 NMO-IgG+补体颅内注射 大脑 单核细胞 6~38 g/L,16.8 μL 12 h/7 d 右转 -
    Asavapanumas等[27] Lewis大鼠 NMO-IgG颅内注射 大脑 中性粒细胞、巨噬细胞 10 μg 5 d - -
    Marignier等[28] 雄性OFA大鼠 NMO-IgG持续颅内注射 大脑、脊髓、视神经 轻微 400 μg 3、7、10、14、21 d 运动协调性变差 -
    Lee等[25] 雌性C57BL/6小鼠 EAE+NMO-IgG+补体重复鞘内注射 大脑、脊髓 - - 0.6 g/L, 10 μL 0~21 d EAE评分增加 -
    Zhang等[26] CD59敲除小鼠 NMO-IgG+补体鞘内注射 脊髓 巨噬细胞/小胶质细胞、中性粒细胞 10 μg 2 d 后肢无力 -
    Geis等[30] 雌性Lewis大鼠 NMO-IgG鞘内注射 脊髓 导管尖端有巨噬细胞轻微浸润 100 g/L或12 g/L,10 μL×15 d 19 d 后肢不对称性麻痹 -
    Harada等[29] Wistar大鼠 NMO-IgG鞘内注射 脊髓 T细胞、小胶质细胞 - 20 μg 0~30 d 后肢麻痹 -
    Ishikura等[23] 雌性Lewis大鼠 抗AQP4重组抗体脊髓注射 脊髓 - - - 20 μg 1~28 d 机械痛阈值降低7 d恢复,热痛阈值无改变
    Zeka等[31] Lewis大鼠 AQP4特异性T细胞转运+NMO-IgG腹腔注射 视神经、大脑、脊髓 T细胞、小胶质细胞和巨噬细胞 10 mg 7 d 尾部张力丧失 -
    Matsumoto等[32] SD大鼠 NMO-IgG视神经鞘下注射 视神经、视网膜 CD11+细胞 - 2 μL 7~14 d - -
    NMOSD: 视神经脊髓炎谱系疾病;AQP4:水通道蛋白4;GFAP:胶质纤维酸性蛋白;EAE:实验性自身免疫性脑脊髓炎;MELFUS:微泡增强低频超声;-:未提及
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  • [1] Wingerchuk DM, Banwell B, Bennett JL, et al. Interna-tional consensus diagnostic criteria for neuromyelitis optica spectrum disorders[J]. Neurology, 2015, 85: 177-189. doi:  10.1212/WNL.0000000000001729
    [2] Lucchinetti CF, Guo Y, Popescu BF, et al. The pathology of an autoimmune astrocytopathy: lessons learned from neuromyelitis optica[J]. Brain Pathol, 2014, 24: 83-97. doi:  10.1111/bpa.12099
    [3] Asseyer S, Cooper G, Paul F. Pain in NMOSD and MOGAD: A Systematic Literature Review of Pathophysiology, Symptoms, and Current Treatment Strategies[J]. Front Neurol, 2020, 11: 778. doi:  10.3389/fneur.2020.00778
    [4] Ayzenberg I, Richter D, Henke E, et al. Pain, Depression, and Quality of Life in Neuromyelitis Optica Spectrum Disorder: A Cross-Sectional Study of 166 AQP4 Antibody-Seropositive Patients[J]. Neurol Neuroimmunol Neuroinflamm, 2021, 8: e985. doi:  10.1212/NXI.0000000000000985
    [5] Kanamori Y, Nakashima I, Takai Y, et al. Pain in neuromyelitis optica and its effect on quality of life: a cross-sectional study[J]. Neurology, 2011, 77: 652-658. doi:  10.1212/WNL.0b013e318229e694
    [6] Qian P, Lancia S, Alvarez E, et al. Association of neuromyelitis optica with severe and intractable pain[J]. Arch Neurol, 2012, 69: 1482-1487. doi:  10.1001/archneurol.2012.768
    [7] Zhao S, Mutch K, Elsone L, et al. Neuropathic pain in neuromyelitis optica affects activities of daily living and quality of life[J]. Mult Scler, 2014, 20: 1658-1661. doi:  10.1177/1352458514522103
    [8] Zhang X, Xu Y, Pei LJ. Review of Neuromyelitis Optica Spectrum Disorder with Pain-Depression Comorbidity[J]. Chin Med Sci J, 2021, 36: 316-322.
    [9] Asseyer S, Cooper G, Paul F. Pain in NMOSD and MOGAD: A Systematic Literature Review of Pathophysiology, Symptoms, and Current Treatment Strategies[J]. Front Neurol, 2020, 11: 778. doi:  10.3389/fneur.2020.00778
    [10] Zhang X, Pei L, Xu Y, et al. Factors correlated with neuropathic pain in patients with neuromyelitis optica spectrum disorder[J]. Mult Scler Relat Disord, 2022, 68: 104213. doi:  10.1016/j.msard.2022.104213
    [11] Li X, Xu H, Zheng Z, et al. The risk factors of neuropathic pain in neuromyelitis optica spectrum disorder: a retrospective case-cohort study[J]. BMC Neurology, 2022, 22: 304. doi:  10.1186/s12883-022-02841-9
    [12] Grace PM, Loram LC, Christianson JP, et al. Behavioral assessment of neuropathic pain, fatigue, and anxiety in experimental autoimmune encephalomyelitis (EAE) and attenuation by interleukin-10 gene therapy[J]. Brain Behav Immun, 2017, 59: 49-54. doi:  10.1016/j.bbi.2016.05.012
    [13] Khan N, Woodruff TM, Smith MT. Establishment and characterization of an optimized mouse model of multiple sclerosis-induced neuropathic pain using behavioral, pharmacologic, histologic and immunohistochemical methods[J]. Pharmacol Biochem Behav, 2014, 126: 13-27. doi:  10.1016/j.pbb.2014.09.003
    [14] Kwilasz AJ, Green Fulgham SM, Duran-Malle JC, et al. Toll-like receptor 2 and 4 antagonism for the treatment of experimental autoimmune encephalomyelitis (EAE)-related pain[J]. Brain Behav Immun, 2021, 93: 80-95. doi:  10.1016/j.bbi.2020.12.016
    [15] Iwamoto S, Itokazu T, Sasaki A, et al. RGMa Signal in Macrophages Induces Neutrophil-Related Astrocytopathy in NMO[J]. Ann Neurol, 2022, 91: 532-547. doi:  10.1002/ana.26327
    [16] Kurosawa K, Misu T, Takai Y, et al. Severely exacerbated neuromyelitis optica rat model with extensive astrocytopathy by high affinity anti-aquaporin-4 monoclonal antibody[J]. Acta Neuropathol Commun, 2015, 3: 82. doi:  10.1186/s40478-015-0259-2
    [17] Hillebrand S, Schanda K, Nigritinou M, et al. Circulating AQP4-specific ao-antibodies alone can induce neuromyelitis optica spectrum disorder in the rat[J]. Acta Neuropathol, 2019, 137: 467-485. doi:  10.1007/s00401-018-1950-8
    [18] Chan KH, Zhang R, Kwan JSC, et al. Aquaporin-4 autoantibodies cause asymptomatic aquaporin-4 loss and activate astrocytes in mouse[J]. J Neuroimmunol, 2012, 245: 32-38. doi:  10.1016/j.jneuroim.2012.02.001
    [19] Kinoshita M, Nakatsuji Y, Kimura T, et al. Neuromyelitis optica: Passive transfer to rats by human immunoglobulin[J]. Biochem Biophys Res Commun, 2009, 386: 623-627. doi:  10.1016/j.bbrc.2009.06.085
    [20] Saini H, Rifkin R, Gorelik M, et al. Passively transferred human NMO-IgG exacerbates demyelination in mouse experimental autoimmune encephalomyelitis[J]. BMC Neurol, 2013, 13: 104. doi:  10.1186/1471-2377-13-104
    [21] Luo J, Xie C, Zhang W, et al. Experimental mouse model of NMOSD produced by facilitated brain delivery of NMO-IgG by microbubble-enhanced low-frequency ultrasound in experimental allergic encephalomyelitis mice[J]. Mult Scler Relat Disord, 2020, 46: 102473. doi:  10.1016/j.msard.2020.102473
    [22] Xiang W, Xie C, Luo J, et al. Low Frequency Ultrasound With Injection of NMO-IgG and Complement Produces Lesions Different From Experimental Autoimmune Encephalomyelitis Mice[J]. Front Immunol, 2021, 12: 727750. doi:  10.3389/fimmu.2021.727750
    [23] Ishikura T, Kinoshita M, Shimizu M, et al. Anti-AQP4 autoantibodies promote ATP release from astrocytes and induce mechanical pain in rats[J]. J Neuroinflammation, 2021, 18: 181. doi:  10.1186/s12974-021-02232-w
    [24] Saadoun S, Waters P, Bell BA, et al. Intra-cerebral injection of neuromyelitis optica immunoglobulin G and human complement produces neuromyelitis optica lesions in mice[J]. Brain, 2010, 133: 349-361. doi:  10.1093/brain/awp309
    [25] Lee CL, Wang KC, Chen SJ, et al. Repetitive intrathecal injection of human NMO-IgG with complement exacerbates disease severity with NMO pathology in experimental allergic encephalomyelitis mice[J]. Mult Scler Relat Disord, 2019, 30: 225-230. doi:  10.1016/j.msard.2019.02.025
    [26] Zhang H, Verkman AS. Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59[J]. J Autoimmun, 2014, 53: 67-77. doi:  10.1016/j.jaut.2014.02.011
    [27] Asavapanumas N, Ratelade J, Verkman AS. Unique neuromyelitis optica pathology produced in naïve rats by intracerebral administration of NMO-IgG [J]. Acta Neuropathol, 2014, 127: 539-551. doi:  10.1007/s00401-013-1204-8
    [28] Marignier R, Ruiza, Cavagna S, et al. Neuromyelitis optica study model based on chronic infusion of autoantibodies in rat cerebrospinal fluid[J]. J Neuroinflammation, 2016, 13: 111. doi:  10.1186/s12974-016-0577-8
    [29] Harada K, Fujita Y, Okuno T, et al. Inhibition of RGMa alleviates symptoms in a rat model of neuromyelitis optica[J]. Sci Rep, 2018, 8: 34. doi:  10.1038/s41598-017-18362-2
    [30] Geis C, Ritter C, Ruschil C, et al. The intrinsic pathogenic role of autoantibodies to aquaporin 4 mediating spinal cord disease in a rat passive-transfer model[J]. Exp Neurol, 2015, 265: 8-21. doi:  10.1016/j.expneurol.2014.12.015
    [31] Zeka B, Hastermann M, Hochmeister S, et al. Highly encephalitogenic aquaporin 4-specific T cells and NMO-IgG jointly orchestrate lesion location and tissue damage in the CNS[J]. Acta Neuropathol, 2015, 130: 783-798. doi:  10.1007/s00401-015-1501-5
    [32] Matsumoto Y, Kanamori A, Nakamura M, et al. Sera from patients with seropositive neuromyelitis optica spectral disorders caused the degeneration of rodent optic nerve[J]. Exp Eye Res, 2014, 119: 61-69. doi:  10.1016/j.exer.2013.12.010
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出版历程
  • 收稿日期:  2022-11-14
  • 录用日期:  2022-12-12
  • 网络出版日期:  2023-01-30
  • 刊出日期:  2023-05-30

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