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A Study on the Invention of Folin-Wu Method and Hsien Wu's Contribution in the Process
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摘要: Folin-Wu法是血糖测定的经典方法之一，是我国著名生化学家吴宪早年的代表性科研成果。本研究通过查阅福林、吴宪等人的原始论文及相关二手史料，追溯并梳理了Folin-Wu法的提出和改进过程，讨论了科研领域的竞争与合作对Folin-Wu法进一步完善的推动作用，旨在基于史料评价Folin-Wu法的影响，并进一步明确吴宪在此方法研究过程中的学术贡献。Abstract: The Folin-Wu method is one of the classic methods for blood glucose determination. It is the representative scientific research achievement of Hsien Wu, one of the most celebrated biochemists in China. This study focuses on the invention process of the Folin-Wu method by referring to the original papers of Folin and Hsien Wu and relevant second-hand historical materials. The implication of competition and cooperation in scientific community on further improvement of the Folin-Wu method is also discussed. The purpose is to evaluate the influence of the Folin-Wu method based on historical data, and to further clarify the academic contribution of Hsien Wu in the research process of this method.
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Key words:
- history of medicine /
- Hsien Wu /
- biochemistry
作者贡献：杜海琳负责文献整理、论文初稿撰写；谷晓阳负责文献检索、修订论文；李嘉珊协助文献整理、内容校对。利益冲突：所有作者均声明不存在利益冲突 -
表 1 Folin-Wu法的主要试剂及改进过程
年份(年) 主要试剂及实验步骤 本尼迪克特的批评 改进方法及优点 改进方法的主要提出者 1919 ①钨酸钠溶液+血液——滤除血液中的蛋白质
②碱性铜试剂(碳酸钠、酒石酸和硫酸铜)——发生铜还原反应
③Folin-Denis试剂(钨酸钠、磷钼酸、磷酸)与苯酚混合——比色定量实验过程中存在亚铜再氧化 ①引入Folin-Wu式血糖管，避免亚铜再氧化
②改良版比色试剂，提高比色准确性吴宪 1920 ①钨酸钠溶液+血液——滤除血液中的蛋白质
②碱性铜试剂(碳酸钠、酒石酸和硫酸铜)——发生铜还原反应
③改良版试剂(磷酸，钨酸钠和钼酸)——比色定量
④反应在Folin-Wu式血糖管内进行测量准确率较低 提高测量的准确性：改良版的铜试剂——为葡萄糖和铜离子反应提供更稳定的环境 福林 1926 ①钨酸钠溶液+血液——滤除血液中的蛋白质
②改良版碱性铜试剂(碳酸氢钠、酒石酸和硫酸铜)——发生铜还原反应
③改良版试剂(磷酸，钨酸钠和钼酸)——比色定量
④反应在Folin-Wu式血糖管内进行无法测定尿糖 - - 
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