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西藏地区藏族人群胃癌组织中H3K27me3表达水平及其临床意义

罗含欢,  师杰,  边巴扎西,  杨旭,  尼玛卓玛,  王倩,  李梅,  王寒,  廖瑞倩,  次仁曲珍

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文章导航 > 协和医学杂志  > 2022 >  13(6): 1088-1093
罗含欢, 师杰, 边巴扎西, 杨旭, 尼玛卓玛, 王倩, 李梅, 王寒, 廖瑞倩, 次仁曲珍. 西藏地区藏族人群胃癌组织中H3K27me3表达水平及其临床意义[J]. 协和医学杂志, 2022, 13(6): 1088-1093. doi: 10.12290/xhyxzz.2021-0727
引用本文: 罗含欢, 师杰, 边巴扎西, 杨旭, 尼玛卓玛, 王倩, 李梅, 王寒, 廖瑞倩, 次仁曲珍. 西藏地区藏族人群胃癌组织中H3K27me3表达水平及其临床意义[J]. 协和医学杂志, 2022, 13(6): 1088-1093. doi: 10.12290/xhyxzz.2021-0727
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LUO Hanhuan, SHI Jie, BIANBA Zhaxi, YANG Xu, NIMA Zhuoma, WANG Qian, LI Mei, WANG Han, LIAO Ruiqian, CIREN Quzhen. Expression of H3K27me3 in Tibetan Patients with Gastric Cancer and Its Significance[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(6): 1088-1093. doi: 10.12290/xhyxzz.2021-0727
Citation: LUO Hanhuan, SHI Jie, BIANBA Zhaxi, YANG Xu, NIMA Zhuoma, WANG Qian, LI Mei, WANG Han, LIAO Ruiqian, CIREN Quzhen. Expression of H3K27me3 in Tibetan Patients with Gastric Cancer and Its Significance[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(6): 1088-1093. doi: 10.12290/xhyxzz.2021-0727
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西藏地区藏族人群胃癌组织中H3K27me3表达水平及其临床意义

doi: 10.12290/xhyxzz.2021-0727
  • 1.

    西藏自治区人民医院病理科，拉萨 850000

  • 2.

    中国医学科学院北京协和医院病理科，北京 100730

  • 3.

    西藏自治区人民医院普外科，拉萨 850000

  • 4.

    中国医学科学院北京协和医院肝脏外科，北京 100730

基金项目: 

西藏自治区自然科学基金组团式援藏医学项目 XZ2017ZR-ZY33

详细信息
    通讯作者:

    师杰, E-mail：shijie@pumch.cn

  • 中图分类号: R365

Expression of H3K27me3 in Tibetan Patients with Gastric Cancer and Its Significance

  • 1.

    Department of Pathology, Tibet Autonomous Region People's Hospital, Lhasa 850000, China

  • 2.

    Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China

  • 3.

    Department of General Surgery, Tibet Autonomous Region People's Hospital, Lhasa 850000, China

  • 4.

    Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China

Funds: 

Tibet Natural Science Funding Committee Grant XZ2017ZR-ZY33

More Information

    摘要
  • 摘要:   目的  探究H3K27me3在西藏地区藏族人群胃癌组织中的表达水平及其与临床、病理特征的相关性。  方法  回顾性收集2019年8月至2021年8月西藏自治区人民医院藏族胃癌患者及同期藏族非胃癌患者的临床与病理资料。采用免疫组化法检测胃癌患者手术切除的肿瘤组织、癌旁正常胃黏膜组织及非胃癌患者正常胃黏膜组织中H3K27me3表达情况，并比较不同临床与病理特征胃癌患者之间H3K27me3表达差异。  结果  共入选符合纳入和排除标准的藏族胃癌患者54例，藏族非胃癌患者55例。H3K27me3定位于细胞核, 阳性表达时细胞核呈棕黄色颗粒状着色。胃癌组织中H3K27me3高表达率显著高于癌旁正常胃黏膜组织[64.8%(35/54)比29.6%(16/54)，P＜0.001]和非胃癌患者正常胃黏膜组织[64.8%(35/54)比34.5%(19/55)，P=0.002]，癌旁正常胃黏膜组织中H3K27me3高表达率与非胃癌患者正常胃黏膜组织无显著差异(P=0.683)。H3K27me3表达水平在不同年龄、性别及Lauren分型、分化程度、浸润深度、肿瘤最大径、TNM分期等胃癌患者之间均无显著差异(P均＞0.05)。  结论  西藏地区藏族人群胃癌组织中H3K27me3表达水平显著增高，暂未发现H3K27me3表达水平与藏族胃癌患者临床及病理特征具有相关性。
    Abstract:   Objective  To investigate the expression and its significance of H3K27me3 in Tibetan patients with gastric cancer.  Methods  Clinical and pathological data were retrospectively collected from Tibetan patients with gastric cancer in the Tibet Autonomous Region People's Hospital from August 2019 to August 2021 and Tibetan non-gastric cancer patients during the same period. The expression of H3K27me3 in gastric cancer tissues, corresponding adjacent normal gastric mucosa and normal gastric mucosa of Tibetan patients with non-gastric cancer tissues was detected by immunohistochemical method, and the differences of H3K27me3 expression between gastric cancer patients with different clinical and pathological characteristics was compared.  Results  A total of 54 Tibetan gastric cancer patients and 55 Tibetan non-gastric cancer patients who met the inclusion and exclusion criteria were enrolled. H3K27me3 was localized in the nucleus, and the nucleus showed brownish-yellow granular staining when positively expressed. The high expression rate of H3K27me3 in gastric cancer tissues was significantly higher than that in adjacent normal gastric mucosa tissue[64.8%(35/54) vs. 29.6%(16/54), P < 0.001]and normal gastric mucosal tissue of Tibetan patients with non-gastric cancer[64.8%(35/54) vs. 34.5%(19/55), P=0.002], and the high expression rate of H3K27me3 in the adjacent normal gastric mucosa tissue was not significantly different from that in normal gastric mucosal tissue of Tibetan patients with non-gastric cancer(P=0.683). H3K27me3 expression in gastric cancer tissues was not related to gender, age, degree of differentiation, depth of invasion, maximum tumor size, Lauren's classification, lymph node metastasis, vascular and nerve invasion and TNM stage.  Conclusions  H3K27me3 is highly expressed in gastric cancer tissues of Tibetan people in Tibet. There is no significant difference in H3K27me3 positive expression cells in adjacent tissues and normal gastric mucosa. The significance of H3K27me3 in Tibetan patients with gastric cancer is uncertain and requires further investigations.
    作者贡献：罗含欢负责试验设计、免疫组化结果判读及论文撰写；师杰负责免疫组化结果判读和论文修订；杨旭进行数据统计学分析；边巴扎西、尼玛卓玛、王倩、王寒、廖瑞倩、次仁曲珍、李梅负责临床病理资料收集、免疫组化制片。
    利益冲突：所有作者均声明不存在利益冲突
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  • 图  1  H3K27me3蛋白在胃癌组织中表达的免疫组化图(EnVision, ×200)

    A.高表达；B.低表达

    下载: 全尺寸图片 幻灯片

    图  2  H3K27me3蛋白在癌旁正常胃黏膜组织中表达的免疫组化图(EnVision，×100)

    A. 高表达；B. 低表达

    下载: 全尺寸图片 幻灯片

    图  3  H3K27me3蛋白在非胃癌患者正常胃黏膜组织中表达的免疫组化图(EnVision，×100)

    A. 高表达；B. 低表达

    下载: 全尺寸图片 幻灯片

    表  1  不同临床与病理特征西藏地区藏族胃癌患者H3K27me3表达情况比较[n(%)]

    指标 低表达(n=19) 高表达(n=35) P值
    年龄 0.128
      ≤60岁 16(84.2) 22(62.9)
      ＞60岁 3(15.8) 13(37.1)
    性别 ＞0.999
      男 13(68.4) 24(68.6)
      女 6(31.6) 11(31.4)
    Lauren分型 0.723
      肠型 6(31.6) 15(42.9)
      弥漫型 7(36.8) 11(31.4)
      混合型 6(31.6) 9(25.7)
    分化程度 0.388
      中-高分化 10(52.6) 13(37.1)
      低分化 9(47.4) 22(62.9)
    浸润深度 0.506
      pT1+pT2 6(31.6) 7(20.0)
      pT3+pT4 13(68.4) 28(80.0)
    肿瘤最大径 0.263
      ≤4 cm 6(31.6) 17(48.6)
      ＞4 cm 13(68.4) 18(51.4)
    脉管侵犯 0.577
      是 7(36.8) 16(45.7)
      否 12(63.2) 19(54.3)
    神经侵犯 0.760
      是 11(57.9) 23(65.7)
      否 8(42.1) 12(34.3)
    淋巴结转移 0.534
      是 12(63.2) 26(74.3)
      否 7(36.8) 9(25.7)
    TNM分期 0.394
      Ⅰ~Ⅱ期 11(57.9) 15(42.9)
      Ⅲ~Ⅳ期 8(42.1) 20(57.1)
    下载: 导出CSV
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  • 收稿日期:  2021-11-02
  • 录用日期:  2021-12-13
  • 刊出日期:  2022-11-30

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