Abstract: The 3rd edition of Formulary of Peking Union Medical College Hospital pressed in 1927 was the earliest collection in our hospital. It was revealed that those common references in English including United States Pharmacopoeia, British Pharmacopoeia, National Formulary (United States), etc., were the most of the bases for the enrollment and quality standard of medications used in our hospital. Both useful and economic characteristics were given priority to the formulary in order to promote the teaching of rational drug therapy, to secure the economy compatible with the welfare of patients, and to limit the number and cost of drugs in stock. It also reflected the unique pharmacy administration, medication-supply system and the pace kept with the international advanced medical level in our hospital nearly a hundred years ago. Its objects, frame, enrolled principles and contents were briefly introduced here.