Abstract: In recent years, research on stem cell has made great progression and widespread clinical application in disease treatments, tissue repair, anti-aging, cosmetology, and other fields. It has become one of the most important researching directions in the field of life science, and has been widely concerned around the world. A high-quality stem cell bank of the clinical grade is not only the precondition for stem cell products to enter clinical practice, but also the key factor for the success of the stem cell industry. Besides, it will provide an indispensable resource for the clinical research of stem cells. Based on the situation of our country and the practice and exploration experience of construction and management of a clinical stem cell bank of East Hospital Affiliated to Tongji University, the article focuses on the planning, construction, and the management of a whole process chain of a stem cell bank of the clinical grade, and covers collection, preparation, testing, cryopreservation, distribution, the establishment of a quality management system, information tracking, and data management of stem cell lifecycle.