Abstract: The role that Musashi-2(Msi-2) protein plays in maintaining the undifferentiated status and self-renewal ability of stem cells has been a hot issue since its discovery. In recent years, it has been confirmed that Msi-2 plays a key role in the development and progression of malignant tumors. The existing study is not limited to Msi-2's function in promoting the development of malignant tumor. The use of Msi-2 in the diagnosis of malignant tumors, and its value in individual treatment have become increasingly prominent. We will review the role of Msi-2 in the development of malignant tumors and its latest research progress as a diagnostic and therapeutic target.