糖化血红蛋白浓度的短期生物学变异

李薇; 黄春梅; 吴卫; 胡莹莹; 陈倩; 郭野; 张玉娟; 崔巍

doi:10.3969/j.issn.1674-9081.2014.03.005

糖化血红蛋白浓度的短期生物学变异

Short-term Biological Variation of Glycated Hemoglobin-A1c

摘要

摘要:
目的研究糖化血红蛋白(glycated hemoglobin-A1c, HbA_1c)浓度短期内的生物学变异。
方法 30名健康志愿者在第1天、第3天和第5天的上午8:00、中午12:00和下午4:00分别采集静脉血, 进行HbA_1c浓度的测定。分析计算HbA_1c浓度的个体内变异(within-subject coefficient of variations, CV_I)、个体间变异(between-subject coefficient of variations, CV_G)、分析变异(analytical coefficient of variations, CV_A)、个体指数(index of individuality, II)和参考变化值(reference change value, RCV)。
结果总体、女性及男性CV_I分别为1.39%、1.49%和1.32%;CV_G分别为2.65%、2.33%和2.90%;CV_A均为0.6%;II分别为0.5、0.6和0.5;95% RCV分别为4.22%、4.45%和4.02%;99% RCV分别为5.19%、5.86%和5.29%。HbA_1c上午8:00、中午12:00和下午4:00的日内变异差异无统计学意义(P=0.28);第1天、第3天和第5天的周内日间变异差异无统计学意义(P=0.31);男女性别间差异亦无统计学意义(P=0.18)。
结论 HbA_1c浓度的日内和周内日间生物学变异很小, 结果相对稳定, 男性和女性之间亦无明显差异。

Abstract:
Objective To study the short-term biological variation of glycated hemoglobin-A1c (HbA_1c).
Methods Blood samples from 30 healthy volunteers 17 men and 13 women; aged 23-34 years (median, 24 years) were drawn at 8:00 am, 12:00 pm, and 4:00 pm each day for 3 days (days 1, 3 and 5), and HbA_1c values were measured in duplicate by high performance liquid chromatography (Variant Ⅱ Yurbo Analyser, Bio-Rad).
Results The within-subject coefficient of variations (CV_I) of total, male, and female subjects in this study were 1.39%, 1.49%, and 1.32%, respectively. The between-subject coefficient of variations (CV_G) of the three groups mentioned above were 2.65%, 2.33%, and 2.90%, respectively. The analytical coefficient of variations (CV_A) of these three groups were all 0.6%. The index of individuality (II) of the three groups were 0.5, 0.6, and 0.5, respectively. The 95% probabilities of reference change value (RCV) of the three groups were 4.22%, 4.45%, and 4.02%, respectively. The 99% probabilities of RCV were 5.19%, 5.86%, and 5.29%, respectively. Furthermore, intra- and inter-day biological variations of HbA_1c were fairly constant among the population examined(P=0.28, 0.31), and no significant difference was observed between the male and female participants(P=0.18).
Conclusions The short-term CV_I and CV_G of HbA_1c in healthy individuals are minimal. The laboratory findings are quite constant and homogeneous for HbA_1c in short term and between genders.

HTML全文

参考文献(6)

施引文献

资源附件(0)