不同剂量和剂量率对宫颈癌细胞的放射敏感性

沈捷; 明越; 汤贤超; 胡克; 刘志凯; 孙显松; 李文博; 杨志伟; 姜菲; 李楠; 李利民; 张福泉

doi:10.3969/j.issn.1674-9081.2013.04.008

不同剂量和剂量率对宫颈癌细胞的放射敏感性

Radiosensitivity of Cervical Cancer Cells to Different Radiation Doses and Dose Rates

摘要

摘要:
目的比较不同类型宫颈癌细胞株在不同剂量和剂量率射线照射下的放射敏感性特点。
方法选取3种宫颈癌细胞株:CaSki、HeLa和SiHa细胞株, 建立3种细胞放射敏感性模型, 运用克隆形成实验分析不同剂量和剂量率照射下3种细胞的放射敏感性及其差别。
结果 3种宫颈癌细胞放射敏感性参数如下:CaSki细胞在2 Gy照射后的存活分数为57.37%, HeLa细胞为70.62%, SiHa细胞为74.77%, 三者差异具有统计学意义(P=0.002)。CaSki、HeLa、SiHa细胞在不同剂量率200、400、600 cGy/min照射后的存活分数差异无统计学意义(P > 0.05)。
结论 3种宫颈癌细胞的放射敏感性以CaSki为最高, HeLa次之, SiHa最低。本研究所用剂量率对3种细胞的存活分数无明显影响。

Abstract:
Objective To explore the radiosensitivity of different types of cervical cancer cells to different radiation doses and dose rates.
Methods We established three cell radiosensitivity models with three cervical cancer cell lines:CaSki, HeLa, and SiHa. We then used clonogenic assay to analyze the radiosensitivity of these three cell lines to different radiation doses and dose rates.
Results The survival fractions at 2 Gy (SF2) were 57.37%, 70.62%, and 74.77% for CaSki, HeLa, and SiHa, respectively (P=0.002). However, the survival fractions did not significantly differ when the three cell lines were treated with dose rates of 200, 400, and 600 cGy/min (P > 0.05).
Conclusions Among these three cell lines, CaSki has the highest radiosensitivity, followed by HeLa and SiHa. The dose rate used in our experiment showed no effect on the the survival fraction of three cervical cancer cell lines.

HTML全文

参考文献(8)

施引文献

资源附件(0)