高渗盐溶液对兔肠缺血/再灌注所致肺损伤的保护作用

徐军; 郑亮亮; 刘业成; 李毅; 朱华栋; 郭树彬; 于学忠; 马遂

doi:10.3969/j.issn.1674-9081.2013.03.007

高渗盐溶液对兔肠缺血/再灌注所致肺损伤的保护作用

Protective Effect of Hypertonic Saline Solution on Lung Injury Induced by Intestinal Ischemia-reperfusion in Rabbits

摘要

摘要:
目的探讨高渗盐溶液(hypertonic saline solution, HS)对肠缺血/再灌注(intestinal ischemia-reperfusion, ⅡR)所致肺损伤的作用。
方法 72只新西兰白兔按随机数字表法分为4组(n=18):空白对照组、ⅡR组、4%HS治疗组、7.5%HS治疗组。空白对照组采用仅开腹、游离但不阻断肠系膜上动脉的假手术处理; 其余实验组采用完全夹闭肠系膜上动脉1 h后开放再灌注的方法制备ⅡR模型, 2个治疗组均在肠系膜上动脉开放前5 min输注相应浓度的氯化钠溶液。选择缺血前与再灌注后2、4、6 h 4个时间点取血测定各组肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)和白细胞介素-10(interleukin-10, IL-10)水平。再灌注后6 h每组处死8只动物, 取其肺组织测定组织中的髓过氧化物酶(myeloperoxidase, MPO)活性、肺组织湿/干比, 光镜下观察病理形态改变。
结果 ⅡR组再灌注后循环中TNF-α和IL-10水平、肺组织MPO活性、肺组织湿/干比以及病理损伤评分均较空白对照组显著升高(P < 0.05);2个HS治疗组与ⅡR组相比, 循环中TNF-α和IL-10水平、肺组织MPO活性、肺组织湿/干比以及病理损伤评分均显著下降(P < 0.05);而7.5%HS组在4%HS组基础上各指标进一步下降(P < 0.05)。
结论 HS治疗对ⅡR所致肺损伤具有保护作用, 该效应在一定范围内随着浓度的增加而增强。

Abstract:
Objective To study the effect of hypertonic saline solution (HS) on lung function in intestinal ischemia-reperfusion (ⅡR) models.
Methods Totally 72 rabbits were randomly divided into 4 groups (n=18):control group, ⅡR group, 4%HS group, and 7.5%HS group. The ⅡR models were produced by clamping super mesenteric artery (SMA) for 1 hour and declamping SMA for 6 hours in ⅡR group, 4%HS group, and 7.5%HS group, whereas animals in control group underwent sham operation. The corresponding sodium chloride solution was infused in the 2 treatment groups in 5 minutes before declamping SMA. Before and 2, 4, 6 hours after ⅡR, the plasma levels of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were measured. At 6 hours after ⅡR, 8 rabbits in each group were killed to measure the levels of lung water and myeloperoxidase (MPO) activity in lung tissue. Meanwhile, lung morphological changes were observed under light microscope.
Results The plasma TNF-α and IL-10 levels, the levels of lung water and MPO activity in lung tissue, and lung morphological score significantly increased in ⅡR group when compared with the control group(P < 0.05). Compared with the ⅡR group, the TNF-α levels in plasma, the levels of lung water and MPO activity in lung tissue, and lung morphological score in both two HS groups significantly decreased (P < 0.05), particularly in 7.5%HS group which was significantly lower than those in the 4%HS group (P < 0.05).
Conclusions HS has a protective effect on the lung function during ⅡR. Such a protective effect increases along with the increase of HS concentration within certain range.

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