直接免疫荧光技术在自身免疫性大疱性皮肤病诊断中的价值

Role of Direct Immunofluorescence in Diagnosis of Autoimmune Bullous Dermatoses: a Retrospective Analysis of 155 Cases

摘要:
目的探讨直接免疫荧光(direct immunofluorescence, DIF)技术在诊断自身免疫性大疱性皮肤病(autoimmune bullous dermatoses, AIBD)中的价值。
方法回顾性分析2009年1月至2011年12月间北京协和医院皮肤科确诊的155例AIBD的DIF检查结果, 总结各类型AIBD的DIF表现特点, 并与组织病理检查对AIBD诊断的敏感性进行比较。
结果各类型AIBD的DIF表现各具特征性。DIF诊断AIBD的敏感性为92.9%, 与组织病理检查敏感性(85.2%)比较差异无统计学意义(χ²=2.063, P=0.058)。
结论 DIF技术敏感性高, 对AIBD的诊断和鉴别诊断有重要意义。

Abstract:
Objective To explore the diagnostic value of direct immunofluorescence (DIF) for autoimmune bullous dermatoses (AIBD).
Methods The DIF features were reviewed in 155 AIBD patients who were admitted to Peking Union Medical College Hospital from January 2009 to December 2011. The diagnostic sensitivity of DIF was compared with that of histopathologic examination.
Results Various types of AIBD showed different DIF features. The diagnostic sensitivity of DIF was 92.9%, which showed no significant difference with that of histopathologic findings (85.2%) (χ²=2.063, P=0.058).
Conclusion DIF plays a significant role in the diagnosis and differential diagnosis of AIBD.