Let-7a和miR-96对胰腺癌K-RAS基因的调节作用

于双妮; 孟云霄; 马怡晖; 卢朝辉; 陈杰

doi:10.3969/j.issn.1674-9081.2012.01.007

Let-7a和miR-96对胰腺癌K-RAS基因的调节作用

Regulatory Effects of Let-7a and miR-96 Molecules on K-RAS in Pancreatic Cancer

摘要

摘要:
目的探讨miRNA分子Let-7a和miR-96对胰腺癌系K-RAS基因的抑制作用及其致病机制。
方法通过检测胰腺癌细胞系miRNA分子(Let-7a和miR-96)水平、K-RAS基因mRNA和蛋白质表达水平, 观察Let-7a和miR-96水平与K-RAS蛋白表达的关系; 并通过升高胰腺癌细胞Let-7a和miR-96水平, 检测其对K-RAS蛋白表达水平的影响。
结果胰腺癌细胞系K-RAS的mRNA与蛋白质表达均保持在一个较高的水平; 胰腺癌细胞系miRNA分子Let-7a和miR-96水平均较低; 升高胰腺癌细胞Let-7a或miR-96水平会降低K-RAS蛋白表达水平。
结论正常状态下, Let-7a和miR-96通过调控K-RAS基因发挥对肿瘤的抑制作用, 胰腺癌细胞的Let-7a和miR-96水平呈一定程度的降低, 削弱了对KRAS基因的抑制作用, 进而参与肿瘤的致病过程。

Abstract:
Objective To explore the effects of Let-7a and miR-96 molecules on inhibition K-RAS in pancreatic cancer.
Methods The levels of K-RAS mRNA and protein were detected in 9 pancreatic cancer cell lines, and the abundance of Let-7a and miR-96 in 7 of these 9 cell lines were determined to analyze the relation between the levels of Let-7a or miR-96 and K-RAS protein expression. Then, the levels of Let-7a or miR-96 was increased in PANC-1 cell line by transfection to observe K-RAS protein change.
Results A high level of K-RAS mRNA was observed in 9 pancreatic cancer cell lines, and K-RAS protein was also at a higher level than normal ones. But Let-7a and miR-96 maintained were at a lower level in pancreatic cancer cell lines. An increase of Let-7a or miR-96 decreased the K-RAS protein expression in PANC-1 cell line.
Conclusion Compared to normal condition, the levels of Let-7a and miR-96 decrease in pancreatic cancer, leading to the attenuation of inhibition to K-RAS, which contributes to pancreatic cancer formation and progression.

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