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两种聚羟基乙酸支架与猪软骨样细胞体外相容性比较

Comparison of Two Different Scaffolds in Terms of Their in vitro Compatibilities with Chondrogenic-like Cells

    摘要
  • 摘要:
      目的  观察猪软骨样细胞与2种不同聚羟基乙酸支架的体外相容性, 为气管组织工程寻求合适的种子细胞载体。
      方法  将猪骨髓间充质干细胞体外诱导分化的软骨样细胞与单纯聚羟基乙酸(polyglycolicacid, PGA)支架和胶原包埋PGA支架体外复合培养, 采用倒置显微镜和扫描电镜观察猪软骨样细胞在2种支架上的生长、黏附情况, MTT法测定软骨样细胞活性, 并与无支架的空白对照比较。
      结果  倒置相差显微镜和扫描电镜示软骨样细胞在胶原包埋PGA支架上吸附、生长良好, 而单纯PGA支架组未见到细胞附着、生长。MTT示软骨样细胞在2种支架材料上与无支架的单纯培养软骨样细胞的活性基本相同。
      结论  胶原包埋PGA支架材料是一种具有良好生物相容性的支架材料, 可做为气管组织工程的种子细胞载体。

     

    Abstract:
      Objective  To study the compatibility of two different scaffolds with chondrogenic-like cells in vitro.
      Methods  Chondrogenic-like cells were derived from bone marrow mesenchymal stem cells. Chondrogenic-like cells and two different scaffolds(PGA scaffold and PGA\collagen composite scaffold) were cocultured in vitro. Morphological features were observed by HE staining and electron microscopy. Cell adhesion and proliferation were detected with MTT assay and compared to the control group.
      Results  Histological and ultrastructural observations showed the cells grew well on the surface of the PGA\collagen composite scaffold. The cells were not adhered on the surface of the PGA scaffold. The MTT assay showed that both two scaffolds did not inhibit the proliferation of chondrogenic-like cells.
      Conclusion  The PGA\collagen composite scaffold has good biocompatibility with chondrogenic-like cells and can be used as a vehicle for trachea tissue engineering.

     

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