Abstract: Objective To summarize the distribution characteristics of human papillomavirus (HPV) infection types in patients with cervical squamous intraepithelial lesion (SIL) and cervical cancer (CC), and to explore the impact of HPV vaccination, HPV infection types, and general clinical data on different grades of cervical lesions. Methods Clinical data of women attending the gynecological colposcopy clinic of Shenzhen People􀆳s Hospital from January 2020 to December 2023 were retrospectively collected. Patients with HPV geno- typing records and histopathologically diagnosed SIL or CC were included and divided into three groups based on pathological results:low-grade squamous intraepithelial lesion (LSIL) group, high-grade squamous intraepi- thelial lesion (HSIL) group, and CC group. The distribution of high-risk HPV subtypes was analyzed among the three groups, and multivariate Logistic regression was used to identify influencing factors for high-grade cer- vical lesions. Results A total of 4162 patients were included, comprising 4057 cervical SIL patients (3317 LSIL and 740 HSIL) and 105 CC patients. The overall mean age was (39.9±11.2) years. The HPV infection rate was 95.1% (3959/4162), and 25.0% (1040/4162) of patients had received HPV vaccination. Among high-risk HPV infections, HPV 52, HPV 16, HPV 58, and HPV 18 were the most common subtypes. HPV 52 had the highest infection rate in the LSIL group (27.6%), while HPV 16 was the most prevalent in the HSIL group (45.3%) and CC group (64.9%). Multivariate Logistic regression analysis showed that HPV vaccination (OR=0.63, 95% CI:0.50-0.79, P<0.001), non-high-risk HPV single infection (OR=0.17, 95% CI:0.09-0.33, P<0.001), HPV 16 single infection (OR=7.05, 95% CI:4.60-10.82, P<0.001), multiple HPV infections (OR=1.63, 95% CI:1.08-2.48, P=0.022), age > 60 years (OR=2.22, 95% CI:1.45-3.40, P<0.001), parity of 2 (OR=1.52, 95% CI:1.14-2.02, P=0.004), and parity ≥ 3 (OR=2.02, 95% CI:1.44-2.84, P<0.001) were influencing factors for high- grade cervical lesions (including HSIL and CC). Conclusions HPV infection is common in patients with SIL and CC, but the distribution of high-risk HPV subtypes varies among different grades of cervical lesions. It is recommended to strengthen cervical cancer screening and monitoring of key high-risk HPV infections in older and multiparous women in Shenzhen, and to continue promoting HPV vaccination.