Abstract: Intestinal tuberculosis (ITB) is a specific infection caused by Mycobacterium tuberculosis invading the intestinal wall. ITB can be categorized into primary and secondary forms, with secondary ITB often resulting from extraintestinal tuberculosis, such as cavitary tuberculosis. Over recent years, there has been an observed increase in ITB incidence. Nevertheless, the early symptoms of patients are mostly inconspicuous and lack specificity. Most progression of the disease is prolonged with slow onset. Therefore, accurate diagnosis of ITB patients is of great significance for the control and prevention of ITB. Currently, the primary laboratory methods for tuberculosis diagnosis include bacteriological examination, histopathological examination, molecular biology diagnosis and imaging techniques. This paper summarizes the progress of various diagnostic methods in ITB diagnosis, with the hope of providing a reference for a wide range of healthcare professionals.