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RSSSLAMF7在小鼠肠道组织及肠道炎症中的表达及意义
Expression and Significance of SLAMF7 in Intestinal Tissue and Intestinal Inflammation in Mice
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摘要:目的 探讨细胞表面受体信号淋巴细胞激活分子家族成员7(signaling lymphocytic activation molecule family member 7, SLAMF7)在小鼠肠道正常组织和炎症组织中的表达及意义。方法 选择8~10周龄C57BL/6J野生型(wild-type)雄性小鼠5只, 正常饮用水喂养, 提取其结肠固有层淋巴细胞(lamina propria lymphocytes, LPLs)和肠上皮细胞(intestinal epithelial cells, IECs), Trizol试剂提取细胞总RNA, 逆转录聚合酶链式反应(reverse transcription polymerase chain reaction, RT-PCR)和实时荧光定量聚合酶链式反应(real-time fluorescence quantitative polymerase chain reaction, RT-qPCR)检测细胞中SLAMF7的mRNA表达情况。选择8~10周龄C57BL/6J野生型雄性小鼠10只, 随机分为对照组和模型组, 每组各5只。对照组用正常饮用水喂养, 模型组用含2.5% 葡聚糖硫酸酯钠盐(dextran sodium sulfate, DSS)的饮用水喂养, 建立小鼠溃疡性结肠炎(ulcerative colitis, UC)模型。于喂养第5天处死两组小鼠, 采用流式细胞术检测对照组与模型组SLAMF7在免疫细胞亚群中的表达情况。结果 相较于结肠IECs, SLAMF7在结肠LPLs中表达显著升高(P=0.017);DSS诱导肠炎后, SLAMF7在中性粒细胞中的表达上调(P=0.001), 在CD4+T淋巴细胞、CD8+T淋巴细胞、CD19+B淋巴细胞、巨噬细胞、经典树突状细胞(conventional dendritic cells, cDCs)中的表达无明显变化(P均＞0.05)。结论 SLAMF7可能通过中性粒细胞相关途径在UC的发生发展过程中发挥重要作用。Abstract:Objective To investigate the expression and significance of cell surface receptor signaling lymphocyte activation molecule family member 7 (SLAMF7) in normal intestinal tissues and intestinal inflammatory tissues of mice.Methods Five C57BL/6J wild-type male mice aged 8-10 weeks were chosen and fed normally. Lamina propria lymphocytes (LPLs) and intestinal epithelial cells (IECs) were extracted, and totalcell RNA was extracted by Trizol reagent. The mRNA expression of SLAMF7 in cells was detected by reverse transcription polymerase chain reaction (RT-PCR) and real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). Then 10 C57BL/6J wild-type male mice aged 8-10 weeks were randomly divided into control group (n=5) and model group (n=5). The control group was fed with normal drinking water, whereas the model group was fed with 2.5% dextran sodium sulfate (DSS) drinking water to establish a model of ulcerative colitis (UC). The mice in both groups were killed on the 5th day. Flow cytometry was used to detect the expression of SLAMF7 in immune cell subpopulations in the control group and the model group.Results Compared with colonic IECs, the expression of SLAMF7 was higher in colonic LPLs(P=0.017). After DSS induced enteritis, the expression of SLAMF7 was up-regulated in neutrophils (P=0.001), but had no significant changes in CD4+T cells, CD8+T cells, CD19+B cells, macrophages and conventional dendritic cells(all P > 0.05).Conclusions SLAMF7 may play an important role in the occurrence and development of UC through neutrophil-related pathways.
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