Abstract: Precision medicine is a medical method that fully considers the differences of individual patients in gene, environment, life style, and so on in the process of diagnosis and treatment. Gene testing is an important way to realize precision medicine. Since the human genome data was analyzed, people have a deeper understanding of the role of genetic factors in the occurrence, development and prognosis of cardiovascular diseases. The emerging precision medicine improves the prevention and treatment of diseases through big data, promotes the progress of genetic research on cardiovascular diseases, and sets the foundation of individualized medicine, which organically combines clinical medicine, basic medicine, and transformational medicine. Genetic variation and gene polymorphism are important factors leading to coronary heart disease. We can not only identify people at high risk of coronary heart disease, but also formulate the best prevention and treatment strategies according to different molecular pathological pathways by genetic detection. All of these approaches promote the application of precision medicine. This review focused on the application of genetic testing in the prevention, diagnosis, and treatment of coronary heart disease and its related diseases.