Abstract: In the past decades, with the rapid development and progress of artificial intelligence (AI), its impact on the field of medicine is deepening. The AI guideline released by the Ministry of Science and Technology of the People's Republic of China also suggested increasing support for the application of AI in medicine. As an important guiding document in medical practice, clinical practice guidelines are of great significance and value in regulating the behavior of healthcare professionals and bridging the gap between the best evidence of research and current practice. How to use AI to accelerate the development process, improve efficiency, innovate the format of dissemination and implementation, and even lead the future of clinical practice guidelines has received more attention from researchers all over the world. This paper analyzed and discussed the current situation and prospects of AI in clinical practice guidelines. We also proposed ideas and suggestions on how to promote the integration of AI and guidelines.