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基于复合多聚体材料的缓释局麻药在慢性痛动物模型中的应用

孙丰润 李默晗 贺渝淼 王涛 马超 黄宇光

孙丰润, 李默晗, 贺渝淼, 王涛, 马超, 黄宇光. 基于复合多聚体材料的缓释局麻药在慢性痛动物模型中的应用[J]. 协和医学杂志, 2022, 13(3): 433-439. doi: 10.12290/xhyxzz.2022-0086
引用本文: 孙丰润, 李默晗, 贺渝淼, 王涛, 马超, 黄宇光. 基于复合多聚体材料的缓释局麻药在慢性痛动物模型中的应用[J]. 协和医学杂志, 2022, 13(3): 433-439. doi: 10.12290/xhyxzz.2022-0086
SUN Fengrun, LI Mohan, HE Yumiao, WANG Tao, MA Chao, HUANG Yuguang. Application of Extended-release Local Anesthetics Based on Composite Polymer Materials in an Animal Model of Chronic Pain[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(3): 433-439. doi: 10.12290/xhyxzz.2022-0086
Citation: SUN Fengrun, LI Mohan, HE Yumiao, WANG Tao, MA Chao, HUANG Yuguang. Application of Extended-release Local Anesthetics Based on Composite Polymer Materials in an Animal Model of Chronic Pain[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(3): 433-439. doi: 10.12290/xhyxzz.2022-0086

基于复合多聚体材料的缓释局麻药在慢性痛动物模型中的应用

doi: 10.12290/xhyxzz.2022-0086
基金项目: 

国家自然科学基金面上项目 81771205

中国博士后科学基金面上项目 2020M680453

详细信息
    通讯作者:

    马超, E-mail:machao@ibms.cams.cn

    黄宇光, E-mail:garypumch@163.com

    孙丰润、 李默晗对本文同等贡献

    孙丰润、 李默晗对本文同等贡献

  • 中图分类号: R614

Application of Extended-release Local Anesthetics Based on Composite Polymer Materials in an Animal Model of Chronic Pain

Funds: 

National Natural Science Foundation of China 81771205

Postdoctoral Science Foundation of China 2020M680453

More Information
  • 摘要:   目的  对复合多聚体材料组成的局麻药缓释载体的药物释放特征和镇痛效果进行测评。  方法  使用静电纺丝技术制备含盐酸布比卡因的PLGA(聚乳酸-羟基乙酸共聚物)缓释膜(静电纺丝膜, M组), 并以PLGA-PEG(聚乙二醇)-PLGA温敏凝胶包裹纺丝膜制备成含盐酸布比卡因的缓释递送系统(凝胶包裹静电纺丝膜形成的复合缓释载体, G组), 评定两组体外释放特征、体内镇痛效果及安全性。  结果  体外条件下, M组对盐酸布比卡因的累积释放时间可达5 d以上, G组累积释放时间可延长至10 d。体内条件下, M组和G组对坐骨神经慢性压迫损伤大鼠模型的镇痛时间均可达14 d, 两组镇痛效果无显著差异(P>0.05)。M组盐酸布比卡因血药浓度在术后第1天达峰值[(0.294±0.029)μg/L], G组于术后第3天达峰值[(0.192±0.064)μg/L], 且峰值较M组降低, 两组盐酸布比卡因血药浓度均在安全范围内。HE染色示, M组、G组大鼠的心脏、肝脏、脾脏、肺、肾脏组织均未见明显病理改变。  结论  本研究制备的以盐酸布比卡因纳米纺丝缓释膜为基础, PLGA-PEG-PLGA温敏凝胶包裹为介质的复合缓释材料, 可进一步延长盐酸布比卡因缓释时间, 具有长效镇痛效果且无毒性作用。
    作者贡献:孙丰润、李默晗负责实验实施、数据处理和论文撰写;贺渝淼、王涛负责实验设计、实验指导和论文修订;马超、黄宇光负责研究设计、论文修订与审核。
    利益冲突:所有作者均声明不存在利益冲突
  • 图  1  静电纺丝扫描电子显微镜观察结果(bar=500 nm)

    A~D分别为15%、20%、25%、30%六氟异丙醇质量体积比

    图  2  溶胶-凝胶转变示意图

    图  3  缓释膜体外释放特征

    M:静电纺丝膜;G:凝胶包裹静电纺丝膜形成的复合缓释载体

    图  4  3组大鼠模型机械痛阈值变化折线图

    *M组与NC组比较,P<0.05;ζM组与NC组比较,P<0.001;#G组与NC组比较,P<0.05; & G组与NC组比较,P<0.001;NC:正常对照; M、G:同图 3

    图  5  盐酸布比卡因血药浓度变化柱状图

    虚线表示盐酸布比卡因的最低致毒血浆浓度
    M、G:同图 3

    图  6  大鼠术后14 d组织病理图(HE,×100)

    NC:同图 4; M、G:同图 3

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出版历程
  • 收稿日期:  2022-02-25
  • 录用日期:  2022-04-11
  • 刊出日期:  2022-05-30

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