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强直性脊柱炎患者肠道菌群特征与疾病活动度及外周血淋巴细胞亚群的相关性

宋子怡 张升校 赵蓉 乔军 宋珊 程婷 王彩虹 李小峰

宋子怡, 张升校, 赵蓉, 乔军, 宋珊, 程婷, 王彩虹, 李小峰. 强直性脊柱炎患者肠道菌群特征与疾病活动度及外周血淋巴细胞亚群的相关性[J]. 协和医学杂志, 2022, 13(5): 821-830. doi: 10.12290/xhyxzz.2022-0064
引用本文: 宋子怡, 张升校, 赵蓉, 乔军, 宋珊, 程婷, 王彩虹, 李小峰. 强直性脊柱炎患者肠道菌群特征与疾病活动度及外周血淋巴细胞亚群的相关性[J]. 协和医学杂志, 2022, 13(5): 821-830. doi: 10.12290/xhyxzz.2022-0064
SONG Ziyi, ZHANG Shengxiao, ZHAO Rong, QIAO Jun, SONG Shan, CHENG Ting, WANG Caihong, LI Xiaofeng. Characteristics of Gut Microbiota and Their Association with Lymphocyte Subsets and Disease Activity in Ankylosing Spondylitis[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(5): 821-830. doi: 10.12290/xhyxzz.2022-0064
Citation: SONG Ziyi, ZHANG Shengxiao, ZHAO Rong, QIAO Jun, SONG Shan, CHENG Ting, WANG Caihong, LI Xiaofeng. Characteristics of Gut Microbiota and Their Association with Lymphocyte Subsets and Disease Activity in Ankylosing Spondylitis[J]. Medical Journal of Peking Union Medical College Hospital, 2022, 13(5): 821-830. doi: 10.12290/xhyxzz.2022-0064

强直性脊柱炎患者肠道菌群特征与疾病活动度及外周血淋巴细胞亚群的相关性

doi: 10.12290/xhyxzz.2022-0064
基金项目: 

国家自然科学基金 82001740

详细信息
    通讯作者:

    李小峰, E-mail: lxf_9859@sxmu.edu.cn

  • 中图分类号: R593.23

Characteristics of Gut Microbiota and Their Association with Lymphocyte Subsets and Disease Activity in Ankylosing Spondylitis

Funds: 

National Natural Science Foundation of China 82001740

More Information
  • 摘要:   目的  探究强直性脊柱炎(ankylosing spondylitis, AS)患者肠道菌群变化特征及其与疾病活动度、外周血淋巴细胞亚群的关系。  方法  本研究为回顾性分析。研究对象为2019年12月至2020年6月山西医科大学第二医院住院治疗的AS患者及性别、年龄与之相匹配的健康人群。收集两组肠道菌群生物信息学分析结果以及AS患者外周血淋巴细胞亚群和疾病活动度指标。比较两组肠道菌群差异,并采用Spearman相关法分析AS患者肠道菌群与疾病活动度、外周血淋巴细胞亚群的相关性。  结果  共入选符合纳入和排除标准的AS患者62例(低、高、极高疾病活动度分别为11例、26例、25例),健康人群62名。α多样性分析显示,AS患者肠道菌群Chao1指数和ACE指数均低于健康人群(P均<0.05);β多样性分析显示,两组菌群结构存在差异(P<0.01)。在肠道微生态构成分析中,发现两组样本肠道菌群优势菌门均以厚壁菌门、拟杆菌门、变形菌门为主,但二者在门和属水平上多种菌群的相对丰度存在差异。在Stamp差异菌群分析中,AS患者在门、属水平亦显示出不同于健康人群的特征:在门水平上,AS患者变形菌门、髌骨细菌门等菌群的相对丰度升高(P均<0.05),厚壁菌门、梭杆菌门等菌群的相对丰度降低(P均<0.05);在属水平上,AS患者大肠杆菌志贺菌属、克雷伯氏菌属、肠球菌属等菌群的相对丰度升高(P均<0.05),普雷沃氏菌属、粪杆菌属等菌群的相对丰度降低(P均<0.05)。Spearman相关性分析表明,AS患者粪杆菌属、瘤胃球菌属及克雷伯氏菌属等菌属的相对丰度与疾病活动度或其相关指标呈正相关(P均<0.05);阿加杆菌属的相对丰度与T细胞(r=0.302,P=0.017)、CD4+T细胞(r=0.310,P=0.014)、B细胞(r=0.292,P=0.021)、Th2细胞(r=0.429,P<0.001)、Th17细胞(r=0.288,P=0.023)水平,链球菌属的相对丰度与B细胞水平(r=0.270,P=0.034),普雷沃氏菌属的相对丰度与Th1细胞(r=0.279,P=0.028)、Th17细胞(r=0.262,P=0.040)水平,CAG-352菌属的相对丰度与Th1细胞水平(r=0.283,P=0.030)均呈正相关;大肠杆菌志贺菌属的相对丰度与Th2细胞水平(r=-0.261,P=0.040),其他肠杆菌科细菌属的相对丰度与CD4+T细胞水平(r=-0.255,P=0.046)均呈负相关。  结论  AS患者肠道菌群多样性降低,致病菌表达增多,且与外周血淋巴细胞亚群和疾病活动度具有相关性,可能参与了AS的发生与发展。
    作者贡献:宋子怡负责选题设计并撰写论文初稿;张升校、乔军负责实验和数据分析;赵蓉、宋珊、程婷负责数据收集和文献查阅;王彩虹、李小峰负责论文修订。
    利益冲突:所有作者均声明不存在利益冲突
  • 图  1  AS患者与健康人群肠道菌群α多样性稀释曲线图

    AS:强直性脊柱炎

    图  2  AS患者与健康人群肠道菌群多样性比较

    A.α多样性;B.β多样性
    AS:同图 1

    图  3  基于Stamp分析和t检验的AS患者与健康人群肠道菌群差异图

    A.门水平;B.属水平
    AS:同图 1

    图  4  差异菌群相对丰度与疾病活动度、外周血淋巴细胞亚群的相关性

    ESR: 红细胞沉降率;CRP: C反应蛋白;ASDAS: 强直性脊柱炎疾病活动度评分;* P<0.05,** P<0.01,*** P<0.001

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出版历程
  • 收稿日期:  2022-02-16
  • 录用日期:  2022-08-02
  • 网络出版日期:  2022-09-06
  • 刊出日期:  2022-09-30

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