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Volume 11 Issue 6
Nov.  2020
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Article Navigation >  Medical Journal of Peking Union Medical College Hospital  > 2020  >  11(6): 649-653
NING Ya-ting, HOU Xin, LU Min-ya, WU Xian, LI Yong-zhe, XU Ying-chun. Application of the Technology of Serum Specific Antibody in Detecting 2019 Novel Coronavirus[J]. Medical Journal of Peking Union Medical College Hospital, 2020, 11(6): 649-653. doi: 10.3969/j.issn.1674-9081.20200050
Citation: NING Ya-ting, HOU Xin, LU Min-ya, WU Xian, LI Yong-zhe, XU Ying-chun. Application of the Technology of Serum Specific Antibody in Detecting 2019 Novel Coronavirus[J]. Medical Journal of Peking Union Medical College Hospital, 2020, 11(6): 649-653. doi: 10.3969/j.issn.1674-9081.20200050
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Application of the Technology of Serum Specific Antibody in Detecting 2019 Novel Coronavirus

doi: 10.3969/j.issn.1674-9081.20200050

  • Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China

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  • Corresponding author: XU Ying-chun  Tel: 86-10-69159766, E-mail: xycpumch@139.com
  • Received Date: 2020-02-25
  • Accepted Date: 2020-03-02
    • Available Online: 2020-03-06
  • Publish Date: 2020-11-30
    Abstract
  • The outbreak of coronavirus disease 2019 in Wuhan in December, 2019 develops rapidly. Early diagnosis of 2019 novel coronavirus (2019-nCoV) infection is the key to epidemic prevention and control. Although a positive nucleic acid test result of 2019-nCoV is the "gold standard" for the diagnosis in those suspected patients, its operation is tedious and time-consuming, and the test results are affected by many factors, such as the sample quality, the infection site and expression level of the virus. Therefore, the single nucleic acid test cannot meet the requirements for rapid screening and diagnosis during the epidemic. Serum specific antibody is another key evidence for the diagnosis of virus infection. Antibody detection can be used as an important means to assist nucleic acid diagnosis and rapid screening. This review interpreted the characteristics of antibody production, the methods and sensitivity/specificity of antibody testing, analysis of false negative and false positive problems, and the combination of the nucleic acid test and antibody detection to promote the establishment and application of the technology of 2019-nCoV serum antibody testing.
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    • References(31)
  • [1] Malik YS, Sircar S, Bhat S, et al. Emerging novel coronavirus (2019-nCoV): current scenario, evolutionary perspective based ongenome analysis and recent developments[J]. Vet Q, 2020, 8: 1-12.
    [2] Lu R, Zhao X, Li J, et al. Genomic characterisation andepidemiology of 2019 novel coronavirus: implications for virusorigins and receptor binding[J].Lancet, 2020, 395:565-574.
    [3] 中华人民共和国国家卫生健康委员会.新型冠状病毒肺炎诊疗方案(试行第六版)[EB/OL].(2020-02-18). http://www.nhc.gov.cn/yzygj/s7653p/202002/8334a8326dd94d329df351d7da8aefc2.shtml.
    [4] Yu F, Du L, Ojcius DM, et al. Measures for diagnosing and treating infections by a novel coronavirus responsible for a pneumonia outbreak originating in Wuhan, China[J]. Microbes Infect, 2020, 22:74-79.
    [5] 何超, 江虹, 谢轶, 等.新型冠状病毒肺炎诊治的实验室检验路径探讨[J].中国呼吸与危重监护杂志, 2020, 19:125-127.
    [6] 黄丹.献血者血液样本核酸检测降低经血传播感染性疾病风险的效果分析[J].泰山医学院学报, 2017, 38: 1043-1044.
    [7] Murat JB, Dard C, Fricker Hidalgo H, et al. Comparison of the vidas system and two recent fully automated assays for diagnosis and follow-up of toxoplasmosis in pregnant women and newborns[J].Clin Vaccine Immunol, 2013, 20: 1203-1212.
    [8] 赵晓飞, 张彩荣, 刘晓娟, 等.表达HIV-1 gag蛋白的重组乳酸乳球菌诱导小鼠产生体液免疫应答[J].细胞与分子免疫学杂志, 2014, 30: 1142-1145.
    [9] 邱峰, 王慧君, 张子康, 等.新型冠状病毒SARS-CoV-2的实验室检测技术[J].南方医科大学学报, 2020, 40:164-167.
    [10] 金晶. ELISA法和化学发光法对血清中HIV-1/HIV-2抗体、梅毒抗体和丙型肝炎抗体的检测分析[J].中国医药指南, 2018, 4: 49.
    [11] 姜大娥.探讨ELISA和化学发光法对血清中HIV-1/HIV-2抗体、梅毒抗体和丙肝抗体的检测[J].中国卫生检验杂志, 2012, 22: 137-138, 145.
    [12] 金建平.单克隆抗体亲和力的测定及其意义[J].生物工程学报, 1987, 3:110-113.
    [13] Li Z, Yi Y, Luo X, et al. Development and Clinical Application of A Rapid IgM-IgG Combined Antibody Test for SARS-CoV-2 Infection Diagnosis[J].J Med Virol, 2020. doi:  10.1002/jmv.25727.[Epub ahead of print].
    [14] 吴敏.基于CdSe/ZnS量子点-荧光免疫层析法对心肌损伤类指标定量快速的检测[D].郑州: 河南大学, 2018.
    [15] 徐秋波, 黄丽娟.吖啶酯直接化学发光法和ELISA定量检测CCP抗体作用分析[J].中外医疗, 2018, 37:191-193.
    [16] 黄小云.提高免疫学检测灵敏度的新型信号传导系统研究[D].南昌: 南昌大学, 2018.
    [17] 王景云.提高免疫层析试纸条灵敏度方法的研究[D].昆明: 云南大学, 2016.
    [18] 张文静.不同粒径金纳米花对双抗夹心免疫层析试纸条检测性能的影响[D].南昌: 南昌大学, 2019.
    [19] Chan JF, Yuan S, Kok KH, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster[J].Lancet, 2020, 395: 514-523.
    [20] Wang J, Wen J, Li J, et al. Assessment of immunoreactive synthetic peptides from the structural proteins of severe acute respiratory syndrome coronavirus[J].Clin Chem, 2003, 49: 1989-1996.
    [21] Lee HK, Lee BH, Dutta NK, et al. Detection of antibodies against SARS-coronavirus using recombinant truncated nucleocapsid proteins by ELISA[J].J Microbiol Biotechnol, 2008, 18: 1717-1721.
    [22] 庄金秋, 梅建国, 张颖, 等.禽流感病毒H7N9亚型实验室检测技术研究进展[J].家禽科学, 2019, 40: 53-59.
    [23] 邰平, 王进. ELISA法检测梅毒抗体假阳性原因分析及其对策[J].江苏预防医学, 2010, 21: 56-57.
    [24] 蒋旭, 李青竹, 邱月红, 等.胶体金免疫层析技术研究进展[J].畜禽业, 2015, 36:28-30.
    [25] 赵玉锋, 张勇, 彭玉芳.化学发光微粒子免疫分析法在HIV检测中假阳性分析[J].医疗装备, 2017, 30: 41.
    [26] Sickinrer E, Jonas G, Yem AW, et al. Performance evaluation of the new full yautomated human immunodeficiency virus antigen-antibody combination assay designed for blow screening[J].Transfusion, 2008, 48: 584-593.
    [27] 方雯丹, 李闻文. HIV感染窗口期及其检测技术的研究[J].检验医学教育, 2012, 3: 43-45.
    [28] Laperche S. Blood safety and nucleicacid testing in Europe[J]. Euro Surveill, 2005, 10: 3-4.
    [29] Fricker-Hidalgo H, Bailly S, Marie-Pierre B, et al. How to estimate time of infection with Toxoplasma gondii in pregnant women. Use of specific IgG and IgM kinetics by 7 techniques on 691 sera[J].Diagn Microbiol Infect Dis, 2020, 15: 114987.
    [30] Phan T. Novel coronavirus: from discovery to clinical diagnostics[J]. Infect Genet Evol, 2020, 79: 104211.
    [31] 刘春华, 孙国清, 薛秀娟, 等.抗体检测与核酸检测在HIV诊断中的优势分析[J].中医临床研究, 2014, 15: 114-116.
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