Expression of ARHI and HuR in PANC-1 Cells

  Objective  To investigate the effect of ARHI transfection on the expression of HuR protein in PANC-1 cells.

  Methods  Plasmids pLNCX2-ARHI-EGFP which express ARHI and empty plasmid pLNCX2-EGFP were used to transfect PANC-1 cells, which were divided into three groups: pLNCX2-ARHI-EGFP group, pLNCX2-EGFP group, and blank control.G418 was used to select stable transfected cell lines. ARHI gene expression was detected by PCR and Western blot, and the expression of HuR protein was detected by Western blot.

  Results  PANC-1 cells transfected with pLNCX2-ARHI-EGFP, the mRNA and protein expression of ARHI were detected. HuR protein expression appeared significantly decreased in PANC-1 cells transfected with pLNCX2-ARHI-EGFP(0.2226±0.0644) compared with the pLNCX2-EGFP-transfected cells (0.4063±0.0925, P=0.029) and PANC-1 blank control cells (0.4178±0.1319, P=0.022).

  Conclusion  ARHI gene in PANC-1 cells may reduce the expression of HuR protein.