Establishing Rat Model of Tibial Cancer Pain with Walker 256 Mammary Gland Carcinoma Cells

  Objective  To explore the feasibility of establishing a rat model of tibial cancer pain with Walker 256 mammary gland carcinoma cells.

  Methods  Totally 36 female SD rats were randomly divided into 4 groups:Sarcoma group (n=8), Sham 1 group (n=10); Sham 2 group (n=10); and Naïve group (n=8). In each treatment group, 105 syngenenic Walker 256 mammary gland carcinoma cells, ascetic fluid without tumor cells, and normal saline were respectively injected into the tibia medullary cavity via intercondylar eminence. The pain behaviors including mechanical paw withdrawal threshold (MWT) and paw withdrawal thermal latency (PWTL), bone radiology, and bone histology were evaluated.

  Results  Observational results via pain behaviors, radiology, and histology demonstrated that rat model of tibial cancer pain was successfully established with Walker 256 mammary gland carcinoma cells. MWT significantly decreased to 37.59±2.02g on the 7th day after tumor cell injection in the Sarcoma group (P < 0.01 compared with Sham 1 group, Sham 2 group, and Naïve group). PWTL significantly decreased to 6.87±1.00s on the 11th day (P < 0.01 compared with Sham 1 group, Sham 2 group, and Naïve group).

  Conclusion  Rat model of tibial cancer pain can be successfully established with Walker 256 mammary gland carcinoma cells.