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Radiosensitivity of Cervical Cancer Cells to Different Radiation Doses and Dose Rates
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摘要:
目的 比较不同类型宫颈癌细胞株在不同剂量和剂量率射线照射下的放射敏感性特点。 方法 选取3种宫颈癌细胞株:CaSki、HeLa和SiHa细胞株, 建立3种细胞放射敏感性模型, 运用克隆形成实验分析不同剂量和剂量率照射下3种细胞的放射敏感性及其差别。 结果 3种宫颈癌细胞放射敏感性参数如下:CaSki细胞在2 Gy照射后的存活分数为57.37%, HeLa细胞为70.62%, SiHa细胞为74.77%, 三者差异具有统计学意义(P=0.002)。CaSki、HeLa、SiHa细胞在不同剂量率200、400、600 cGy/min照射后的存活分数差异无统计学意义(P > 0.05)。 结论 3种宫颈癌细胞的放射敏感性以CaSki为最高, HeLa次之, SiHa最低。本研究所用剂量率对3种细胞的存活分数无明显影响。 Abstract:Objective To explore the radiosensitivity of different types of cervical cancer cells to different radiation doses and dose rates. Methods We established three cell radiosensitivity models with three cervical cancer cell lines:CaSki, HeLa, and SiHa. We then used clonogenic assay to analyze the radiosensitivity of these three cell lines to different radiation doses and dose rates. Results The survival fractions at 2 Gy (SF2) were 57.37%, 70.62%, and 74.77% for CaSki, HeLa, and SiHa, respectively (P=0.002). However, the survival fractions did not significantly differ when the three cell lines were treated with dose rates of 200, 400, and 600 cGy/min (P > 0.05). Conclusions Among these three cell lines, CaSki has the highest radiosensitivity, followed by HeLa and SiHa. The dose rate used in our experiment showed no effect on the the survival fraction of three cervical cancer cell lines. -
Key words:
- cervical cancer /
- radiosensitivity /
- radiotherapy
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表 1 CaSki、HeLa和SiHa细胞照射后单靶多击数学模型拟合细胞存活曲线参数
细胞 PE D0 N SF2 Dq 曲线拟合度 CaSki 0.606222 2.0576 1.794 0.5737* 1.202† 0.9993 HeLa 0.685778 2.5458 2.014 0.7062* 1.782† 0.9936 SiHa 0.756444 1.6989 3.737 0.7477* 2.240† 0.9999 PE：贴壁率；D0：平均致死量；N：外推数；SF2：2 Gy照射的细胞存活分数；Dq：准域剂量; 三种细胞间比较，*P=0.005，†P=0.002 
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表 2 CaSki、HeLa和SiHa细胞不同剂量率照射后存活分数(x±s)
细胞 0 cGy/min 200 cGy/min 400 cGy/min 600 cGy/min CaSki 1.00±0.00(1.00~1.00) 0.52±0.15(-0.85~1.88) 0.45±0.06(-0.07~0.97) 0.47±0.06(-0.86~1.02) HeLa 1.00±0.00(1.00~1.00) 0.53±0.17(80.49~0.57) 0.48±0.02(0.45~0.52) 0.42±0.16(0.26~0.58) SiHa 1.00±0.00(1.00~1.00) 0.74±0.03(0.66~0.82) 0.62±0.03(0.55~0.69) 0.57±0.07(0.40~0.73)
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