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目的 观察二期梅毒患者外周血中白细胞介素-2(interleukin-2, IL-2)的水平。 方法 收集2010年9月至12月在北京协和医院皮肤科性病中心就诊的二期梅毒患者26例, 其中男20例, 女6例, 年龄16~76岁, 平均(40±13)岁, 所有病例均经快速血浆反应素环状卡片试验(rapid plasma reagin, RPR)和梅毒螺旋体明胶凝结试验确诊; 应用酶联免疫吸附试验检测26例二期梅毒患者和14名年龄匹配的健康对照者血清IL-2的水平。 结果 二期梅毒患者血清IL-2水平较正常对照者明显升高[分别为(2.510±0.529) pg/ml和(2.225±0.157) pg/ml, P=0.016];而二期梅毒患者年龄、性别、是否接受治疗、IgM是否阳性及RPR滴度对IL-2水平均无明显影响。 结论 梅毒螺旋体入侵可能引起早期梅毒患者外周血中IL-2水平升高, 增强宿主对梅毒螺旋体的免疫反应, 有利于梅毒螺旋体的清除。 Abstract:Objective To observe the change of interleukin-2 (IL-2) levels in the peripheral blood of patients with secondary syphilis. Methods Totally 26 patients (20 males and 6 females) with acquired syphilis aged 16-76 years (mean 40±13 years), who were treated in the Sexually Transmitted Disease Clinic of Peking Union Medical College Hospital from September to December 2010, were enrolled in this study. Definite diagnosis was based on the results of rapid plasma reagin (RPR) and Treponema pallidum particle agglutination (TPPA) assay. Serum IL-2 level was detected by enzyme-linked immunosorbent assay (ELISA) in these patients and 14 age-matched uninfected volunteers (controls). Results Compared with the controls, serum IL-2 level significantly increased in patients with secondary syphilis[(2.510±0.529) pg/ml vs. (2.225±0.157) pg/ml, P=0.016]. However, there were no significant differences between the secondary syphilis patients grouped with different age, gender, treatment, IgM, or RPR titers. Conclusions The invasion of Treponema pallidum induces the increase of serum IL-2 levels in patients with early syphilis, which may reinforce the immune response against Treponema pallidum and facilitate the clearance of this pathogen. -
Key words:
- syphilis /
- interleukin-2
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表 1 不同组间IL-2水平的比较
组别 例数 IL-2水平(pg /ml，x±s) t值 P值 分组 正常对照组 14 2. 225 ± 0. 157 2. 540 0. 016 二期梅毒组 26 2. 510 ± 0. 529 年龄 ≤40岁 12 2. 679 ± 0. 713 1. 457 0. 169 ＞40岁 14 2. 365 ± 0. 242 性别 男 20 2. 581 ± 0. 580 1. 270 0. 216 女 6 2. 272 ± 0. 181 治疗情况 未治疗 13 2. 325 ± 0. 299 － 1. 863 0. 075 治疗后 13 2. 694 ± 0. 648 IgM 阳性 18 2. 555 ± 0. 618 0. 642 0. 527 阴性 8 2. 409 ± 0. 236 RPR滴度 ≥1:8 14 2. 394 ± 0. 325 1. 217 0. 236 阴性 8 2. 409 ± 0. 236 ＜1:8 12 2. 645 ± 0. 689 
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