Histological Evaluation of Skin In Vitro Treated by Noval Er: YSGG Ablative Fractional Laser
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摘要:
目的 评估新型剥脱性饵:钇钪镓石榴石(erbium-doped yttrium scandium gallium garnet, Er:YSGG)2790 nm激光治疗不同深度的表皮和真皮组织获得的剥脱效果。 方法 收集手术切除的16例新鲜离体人体皮肤标本, 包括面部、颈部、胸部、上肢屈侧部位各4例, 采用Er:YSGG 2790 nm激光照射聚焦于300 μm直径光斑大小, 能量为每光斑60~160 mJ, 扫描面积10 mm × 10 mm, 在离体皮肤上形成微阵列模式。通过HE染色观察损伤的深度和宽度, 评估不同的脉冲能量对不同皮肤组织的治疗效果。 结果 Er:YSGG 2790 nm激光可在离体人体皮肤上形成剥脱性热损伤微阵列, 用不同能量可选择性到达皮肤的不同深度(99~456 μm), 包括表皮、真皮浅层、中层和深层, 腔隙间距为294~405 μm, 腔隙焦化内壁厚度为22~77 μm。 结论 新型剥脱性Er:YSGG 2790 nm激光以点阵方式操作, 可在人体不同组织中产生热反应。使用不同的脉冲能量治疗, 可以在表皮和真皮层形成不同深度的微阵列模式。 Abstract:Objective To evaluate the ablative effects of a noval ablative erbium-doped yttrium scandium gallium garnet (Er:YSGG) 2790-nm laser treatment at different depths of the epidermis and dermis tissue. Methods Freshly isolated human skin specimens were obtained from surgically resected face, neck, chest, and upper limb flexor side tissues in 16 patients. The specimens were irradiated with Er:YSGG 2790-nm laser, with a focused spot diameter of 300 μm, an energy of 60-160 mJ, and a scanning area of 10 mm × 10 mm. Microarray mode was established in the skin in vitro. The depth and width of the damage were assessed by HE staining. The therapeutic effectiveness of different pulse energies on different skin tissues were evaluated. Results After the irradiation of Er:YSGG 2790-nm laser, ablative fractional heat damage microarrays formed in the human skin in vitro. Different depths of skin (99-456 μm), including the epidermis, superficial dermis, and deep dermis, were selectively reached when the laser was applied at different pulse energies. The cavities were separated for 294-405 μm, and the inner coking walls of cavity ranged 22-77 μm. Conclusions The no-val ablative Er:YSGG 2790-nm laser, when applied in fractional operations, can create thermal reaction in different body tissues. Treatment with different pulse energies, the microarray mode can be formed in the epidermis and dermis layers of different depths. -
表 1 不同Er: YSGG激光能量作用于不同部位皮肤的深度(μm)
能量(mJ) 面部60 颈部 胸部 上肢屈侧 60 99 ~ 105 120 ~ 125 118 ~ 124 180 ~ 190 80 287 ~ 299 149 ~ 154 205 ~ 209 230 ~ 235 120 345 ~ 354 182 ~ 190 235 ~ 245 317 ~353 160 450~456 260~270 269~276 385~391 表 2 不同Er: YSGG激光能量作用于不同部位皮肤的腔隙间距(μm)
能量(mJ) 面部60 颈部 胸部 上肢屈侧 60 353 ~ 360 344 ~ 374 321~ 356 294 ~ 372 80 359 ~ 387 335~ 398 344 ~ 368 389 ~ 401 120 372 ~ 398 365~ 378 357 ~ 398 311 ~369 160 380~405 389~404 381~398 386~391 表 3 不同Er: YSGG激光能量作用于不同部位皮肤的焦化内壁厚度(μm)
能量(mJ) 面部60 颈部 胸部 上肢屈侧 60 22 ~ 45 35 ~ 37 26~ 29 37 ~ 41 80 55 ~ 58 31~ 37 33 ~ 36 57 ~ 60 120 70 ~ 72 42~ 44 40 ~ 45 56 ~63 160 71~77 70~72 52~57 70~74 表 4 不同Er: YSGG激光脉冲能量对皮肤组织的平均损伤深度
能量(mJ) 面部60 颈部 胸部 上肢屈侧 60 表皮 表皮 表皮 表皮 80 真皮浅层 真皮浅层 表皮或真皮浅层 表皮或真皮浅层 120 真皮中层 真皮中层 真皮中层 真皮中层 160 真皮深层 真皮深层 真皮中层 真皮中层 -
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