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Cyclin D1在乳腺浸润性小叶癌的表达及其意义

师杰,  梁智勇,  刘彤华

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文章导航 > 协和医学杂志  > 2012 >  3(1): 8-12
师杰, 梁智勇, 刘彤华. Cyclin D1在乳腺浸润性小叶癌的表达及其意义[J]. 协和医学杂志, 2012, 3(1): 8-12. doi: 10.3969/j.issn.1674-9081.2012.01.004
引用本文: 师杰, 梁智勇, 刘彤华. Cyclin D1在乳腺浸润性小叶癌的表达及其意义[J]. 协和医学杂志, 2012, 3(1): 8-12. doi: 10.3969/j.issn.1674-9081.2012.01.004
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Jie SHI, Zhi-yong LIANG, Tong-hua LIU. Expression of Cyclin D1 in Invasive Lobular Carcinoma of the Breast and Its Significance[J]. Medical Journal of Peking Union Medical College Hospital, 2012, 3(1): 8-12. doi: 10.3969/j.issn.1674-9081.2012.01.004
Citation: Jie SHI, Zhi-yong LIANG, Tong-hua LIU. Expression of Cyclin D1 in Invasive Lobular Carcinoma of the Breast and Its Significance[J]. Medical Journal of Peking Union Medical College Hospital, 2012, 3(1): 8-12. doi: 10.3969/j.issn.1674-9081.2012.01.004
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Cyclin D1在乳腺浸润性小叶癌的表达及其意义

doi: 10.3969/j.issn.1674-9081.2012.01.004

  • 中国医学科学院 北京协和医学院 北京协和医院病理科, 北京 100730

详细信息
    通讯作者:

    刘彤华 电话:010-65295523, E-mail:liuthp-umch@yah

  • 中图分类号: R737.9;R365

Expression of Cyclin D1 in Invasive Lobular Carcinoma of the Breast and Its Significance

  • Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China

More Information
    Corresponding author: LIU Tong-hua Tel: 010-65295523, E-mail:liuthp-umch@yah

    摘要
  • 摘要:   目的  探讨Cyclin D1在乳腺浸润性小叶癌中的表达及其意义。  方法  采用免疫组化染色方法对75例具有经典型乳腺浸润性小叶癌结构的标本进行Cyclin D1免疫组化检测。  结果  Cyclin D1阳性表达率为93.33%(70/75), 其中41例(54.67%)呈弥漫强阳性表达(3+, 6~7分), 21例(28%)呈中阳性表达(2+, 4~5分), 8例(10.67%)呈弱阳性表达(1+, 2~3分); 仅有5例(6.67%)呈阴性表达(0~1分)。Cyclin D1的阳性表达在患者年龄是否≥ 50岁、肿瘤最大径是否≥ 2 cm、淋巴结是否有转移、是否累及乳头间质及其下大导管、雌孕激素受体有无及P53是否突变等因素的分组中无统计学差异(P > 0.05);而Cyclin D1阳性表达程度与淋巴结是否有转移具有一定的负相关性(P < 0.05)。  结论  Cyclin D1在乳腺浸润性小叶癌中呈过表达状态, 该蛋白过表达提示其在基因水平上可能出现了某种程度的异常, 这一假设尚需进一步行基因检测加以证实。
    Abstract:   Objective  To investigate the expression and its significance of Cyclin D1 in invasive lobular carcinoma (ILC) of the breast.  Methods  The specimens from 75 cases of ILC were detected using immunohistochemical method.  Results  Of these 75 cases, Cyclin D1 was positive on the nucleus in 70 cases (93.33%), among which 41 (54.67%) showed strong diffuse staining (3 +, 6-7 points), and 21 (28%) were moderately positive (2 +, 4-5 points), and 8 (10.67%) were weak positive (1 +, 2-3 points). Only 5 (6.67%) cases showed negative results (0-1 points). The positive expression of Cyclin D1 showed no significant differences among patients in terms of age (> 50 years), maximum tumor diameter (> 2cm), lymph node metastasis, involvement of papillary interstitial tissue and the large duct beneath it, existence of estrogen and progesterone receptors, and the possible mutation of P53. However, an inverse correlation was found between the degrees of the positive staining of Cyclin D1 and the lymph node metastasis (P < 0.05).  Conclusion  Cyclin D1 is overexpressed in ILC, suggesting that there may be certain genetic abnormalities.
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  • 图  1  单一形态的小细胞呈单排线性排列或围绕导管呈环靶状排列(HE, ×40)

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    图  2  高倍镜下肿瘤细胞形态均一, 单个散在或单排线性浸润在间质中(HE, ×200)

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    图  3  Cyclin D1在肿瘤细胞核呈弥漫强阳性表达(3 +, 7分, ×40), 肿瘤组织内残存正常小导管呈阴性表达

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    图  4  Cyclin D1在肿瘤细胞核呈弥漫强阳性表达(3 +, ×100)

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    图  5  Cyclin D1在肿瘤细胞核呈中等强度阳性表达(2 +, ×100)

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    图  6  Cyclin D1在肿瘤细胞核呈弱阳性表达(1 +, ×100)

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    图  7  Cyclin D1在肿瘤细胞核呈阴性表达(-, ×100)

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    表  1  Cyclin D1阳性与临床、病理特征的关系

    表  2  Cyclin D1阳性表达程度与临床、病理特征的相关性
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  • 收稿日期:  2011-10-25
  • 刊出日期:  2012-01-30

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