Comparison of Two Different Scaffolds in Terms of Their in vitro Compatibilities with Chondrogenic-like Cells
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摘要:
目的 观察猪软骨样细胞与2种不同聚羟基乙酸支架的体外相容性, 为气管组织工程寻求合适的种子细胞载体。 方法 将猪骨髓间充质干细胞体外诱导分化的软骨样细胞与单纯聚羟基乙酸(polyglycolicacid, PGA)支架和胶原包埋PGA支架体外复合培养, 采用倒置显微镜和扫描电镜观察猪软骨样细胞在2种支架上的生长、黏附情况, MTT法测定软骨样细胞活性, 并与无支架的空白对照比较。 结果 倒置相差显微镜和扫描电镜示软骨样细胞在胶原包埋PGA支架上吸附、生长良好, 而单纯PGA支架组未见到细胞附着、生长。MTT示软骨样细胞在2种支架材料上与无支架的单纯培养软骨样细胞的活性基本相同。 结论 胶原包埋PGA支架材料是一种具有良好生物相容性的支架材料, 可做为气管组织工程的种子细胞载体。 Abstract:Objective To study the compatibility of two different scaffolds with chondrogenic-like cells in vitro. Methods Chondrogenic-like cells were derived from bone marrow mesenchymal stem cells. Chondrogenic-like cells and two different scaffolds(PGA scaffold and PGA\collagen composite scaffold) were cocultured in vitro. Morphological features were observed by HE staining and electron microscopy. Cell adhesion and proliferation were detected with MTT assay and compared to the control group. Results Histological and ultrastructural observations showed the cells grew well on the surface of the PGA\collagen composite scaffold. The cells were not adhered on the surface of the PGA scaffold. The MTT assay showed that both two scaffolds did not inhibit the proliferation of chondrogenic-like cells. Conclusion The PGA\collagen composite scaffold has good biocompatibility with chondrogenic-like cells and can be used as a vehicle for trachea tissue engineering. -
Key words:
- chondrogenic-like cells /
- scaffold /
- biocompatibility /
- porcine
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图 2 扫描电镜所见胶原包埋PGA支架 SEM ×500
PGA:同图 1
图 3 倒置显微镜下胶原包埋PGA细胞种植后7天的形态HE染色10 × 10
PGA:同图 1
图 4 扫描电镜示软骨样细胞在胶原包埋PGA支架表面黏附、生长 SEM × 2300
PGA:同图 1
表 1 单纯PGA组、胶原包埋PGA组及空白对照组光吸收值比较
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[1] Bjerre L, Bunger CE, Kassem M, et al.Flow perfusion culture of human mesenchymal stem cells on silicate substituted tricalcium phosphate scaffolds[J].Biomaterials, 2008, 29:2616-2627. doi: 10.1016/j.biomaterials.2008.03.003 [2] 韩志军, 刘晓峥, 任华.猪骨髓间质干细胞分离培养及向成软骨细胞表型诱导分化的实验研究[J].中国胸心血管外科临床杂志, 2007, 14:354-357. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgxxxgwk98200705009 [3] Wang L, Tran I, Seshareddy K, et al.A comparison of human bone marrow-derived mesenchymal stem cells and human umbilical cord-derived mesenchymal stromal cells for cartilage tissue engineering[J].Tissue Eng Part A, 2009, 15:2259-2266. doi: 10.1089/ten.tea.2008.0393 [4] Butler DL, Gooch C, Kinneberg KR, et al.The use of mesenchymal stem cells in collagen-based scaffolds for tissue-engineered repair of tendons[J].Nat Protoc, 2010, 5:849-863. doi: 10.1038/nprot.2010.14 [5] Tan H, Wu J, Lao L, et al.Gelatin/chitosan/hyaluronan scaffold integrated with PLGA microspheres for cartilage tissue engineering[J].Actaiomaterialia, 2009, 5:328-337. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=f6c6b3c9bc87be3d958effc4101ec12a [6] Kawazoe N, Chen G, Tateishi T.Developmentof novel biomaterials for bone and cartilage tissue engineering[J].Clin Calcium, 2008, 18:1713-1720. https://www.ncbi.nlm.nih.gov/pubmed/19043184 [7] Karageorgiou V, Kaplan D.Porosity of 3D biomaterial scaffolds and osteogenesis[J].Biomaterials, 2005, 26:5474-5491. doi: 10.1016/j.biomaterials.2005.02.002 [8] 初殿伟, 王玮, 张华亮, 等.骨软骨组织工程支架的研究现状及发展趋势[J].国际生物医学工程杂志, 2009, 32:105-107. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gwyx-swyxgc200902011 [9] Butler DL, Gooch C, Kinneberg KR, et al.The use of mesenchymal stem cells in collagen-based scaffolds for tissue-engineered repair of tendons[J].Nat Protoc, 2010, 5:849-863. doi: 10.1038/nprot.2010.14 [10] Lu H, Ko YG, Kawazoe N, et al.Cartilage tissue engineering using funnel-like collagen sponges prepared with embossing ice particulate templates[J].Biomaterials, 2010, 31:5825-5835. doi: 10.1016/j.biomaterials.2010.04.019 [11] Cho SW, Jeon O, Lim JE, et al.Preliminary experience with tissue engineering of a venous vascular patch by using bone marrowderived cells and a hybrid biodegradable polymer scaffold[J].J Vasc Surg, 2006, 44:1329-1340. doi: 10.1016/j.jvs.2006.07.032 [12] Hannouche D, Terai H, Fuchs JR, et al.Engineering of implantable cartilaginous structures from bone marrow derived mesenchymal stem cells[J].Tissue Eng, 2007, 13:87-99. doi: 10.1089/ten.2006.0067