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两种聚羟基乙酸支架与猪软骨样细胞体外相容性比较

韩志军 任华

韩志军, 任华. 两种聚羟基乙酸支架与猪软骨样细胞体外相容性比较[J]. 协和医学杂志, 2011, 2(1): 42-45. doi: 10.3969/j.issn.1674-9081.2011.01.009
引用本文: 韩志军, 任华. 两种聚羟基乙酸支架与猪软骨样细胞体外相容性比较[J]. 协和医学杂志, 2011, 2(1): 42-45. doi: 10.3969/j.issn.1674-9081.2011.01.009
Zhi-jun HAN, Hua REN. Comparison of Two Different Scaffolds in Terms of Their in vitro Compatibilities with Chondrogenic-like Cells[J]. Medical Journal of Peking Union Medical College Hospital, 2011, 2(1): 42-45. doi: 10.3969/j.issn.1674-9081.2011.01.009
Citation: Zhi-jun HAN, Hua REN. Comparison of Two Different Scaffolds in Terms of Their in vitro Compatibilities with Chondrogenic-like Cells[J]. Medical Journal of Peking Union Medical College Hospital, 2011, 2(1): 42-45. doi: 10.3969/j.issn.1674-9081.2011.01.009

两种聚羟基乙酸支架与猪软骨样细胞体外相容性比较

doi: 10.3969/j.issn.1674-9081.2011.01.009
详细信息
    通讯作者:

    任华  电话:010-65296046, E-mail:renjohnh@yahoo.com.cn

  • 中图分类号: R318.08

Comparison of Two Different Scaffolds in Terms of Their in vitro Compatibilities with Chondrogenic-like Cells

More Information
  • 摘要:   目的  观察猪软骨样细胞与2种不同聚羟基乙酸支架的体外相容性, 为气管组织工程寻求合适的种子细胞载体。  方法  将猪骨髓间充质干细胞体外诱导分化的软骨样细胞与单纯聚羟基乙酸(polyglycolicacid, PGA)支架和胶原包埋PGA支架体外复合培养, 采用倒置显微镜和扫描电镜观察猪软骨样细胞在2种支架上的生长、黏附情况, MTT法测定软骨样细胞活性, 并与无支架的空白对照比较。  结果  倒置相差显微镜和扫描电镜示软骨样细胞在胶原包埋PGA支架上吸附、生长良好, 而单纯PGA支架组未见到细胞附着、生长。MTT示软骨样细胞在2种支架材料上与无支架的单纯培养软骨样细胞的活性基本相同。  结论  胶原包埋PGA支架材料是一种具有良好生物相容性的支架材料, 可做为气管组织工程的种子细胞载体。
  • 图  1  倒置显微镜下单纯PGA无纺网状纤维支架  IM 10×10

    PGA:聚羟基乙酸

    图  2  扫描电镜所见胶原包埋PGA支架  SEM ×500

    PGA:同图 1

    图  3  倒置显微镜下胶原包埋PGA细胞种植后7天的形态HE染色10 × 10

    PGA:同图 1

    图  4  扫描电镜示软骨样细胞在胶原包埋PGA支架表面黏附、生长  SEM × 2300

    PGA:同图 1

    表  1  单纯PGA组、胶原包埋PGA组及空白对照组光吸收值比较

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出版历程
  • 收稿日期:  2010-09-28
  • 刊出日期:  2011-01-30

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