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Correlation of Memory B Cell and B Cell Activation Factor with Clinical Indicators in Primary Sj?gren's Syndrome Patients and Their First-degree Relatives
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摘要:
目的 评价干燥综合征患者及一级亲属记忆性B细胞(CD19+CD27+B细胞)及B细胞激活因子(Bcell activation factor, BAFF)的外周表达及其与临床指标的相关性。 方法 以北京协和医院确诊的原发性干燥综合征(primary Sjögren’s syndrome, pSS)患者41例、患者一级亲属7名及正常健康献血者27名的外周血为标本, 用流式细胞仪分析记忆性B细胞, 用ELISA法检测血浆B细胞激活因子(BAFF)水平, 结合患者临床资料进行分析。 结果 原发性干燥综合征患者记忆性B细胞数目较正常人显著升高(11.10%±5.10% vs.9.24%±6.99%, P=0.0002), BAFF水平亦较正常人显著升高[(1.18±0.72)ng/ml vs.(0.43±0.19)ng/ml, P=0.0005];患者与其一级亲属的记忆性B细胞数目无显著性差异(16.26%±8.30% vs.8.75%±3.82%, P=0.088), 患者的BAFF水平与其一级亲属比较显著升高[(1.29±0.46)ng/ml vs.(0.56±0.11)ng/ml, P=0.015]。结合临床资料分析, 发现记忆性B细胞与5-min未刺激唾液量呈正相关(r=0.345, P=0.027), 与眼科角结膜评分呈负相关(r=-0.321, P=0.041), 与血清IgM、IgA、IgG、补体C3、补体C4无相关关系(P > 0.05)。BAFF与血清IgM、IgA、IgG、补体C3、补体C4、口腔科灶性指数、眼科角结膜评分无相关关系(P>0.05)。 结论 记忆性B细胞在患者及其一级亲属高表达, 与临床客观指标评价相反, 提示记忆性B细胞在原发性干燥综合征疾病发生发展过程中可能存在从外周向腺体组织的迁移; 患者BAFF水平明显高于正常人及其一级亲属, 与临床指标不相关, 表明BAFF可能参与原发性干燥综合征发生后的一系列反应而并非直接引起疾病, 并且其水平尚不足以反映疾病的严重程度。 Abstract:Objectives To investigate the expression of CD19+CD27+ (memory B cell) and B cell activation factor(BAFF), in the peripheral blood of patients with primary Sjögren's syndrome (pSS) and their relatives and to analyze the correlations of the cells with clinical indicator. Methods Peripheral venous bloodsamples were collected from 41 newly diagnosed pSS patients[females, age 48.7±9.1 years, anti-SSA/SSB (+) 73.2%] from Sjögren's International Collaborative Clinical Alliance(SICCA) and Peking Union Medical College Hospital(PUMCH), and 27 healthy controls (females, age 47.0±3.8 years) and 7 first-degree relatives of the patients (females, age 50.1±5.0 years). The levels of memory B cells in the peripheral blood were measured by flow-cytometric. The level of BAFF in serum was determined with enzyme-linked immunosorbent assay (ELISA). Clinical indicators including IgG, IgA, IgM, C3, C4, Focus score, 5-min salivary rate, ocular score, Schirmer test results were tested simultaneously. Results The level of memory B cell in the blood of pSS patients was 11.10%±5.10%, which was significantly higher than that of the healthy controls 9.24%±6.99% (P=0.0002), but was not significant differences between pSS patients and their first-degree relatives (P>0.05). BAFF in pSS was 1.18±0.72 ng/ml, which was significantly higher than that of the healthy controls (0.43±0.19 ng/ml) (P=0.0005) and than that of their first-degree relatives (0.56±0.11 ng/ml) (P=0.015). CD27+ B cell were positively correlated with 5-min salivary rate (r=0.345, P=0.027), negatively correlated with ocular score (r=-0.321, P=0.041); however, it was not correlated with IgG, IgA, IgM, C3, C4, focus score, and Schirmer test results. BAFF was not correlated with IgG, IgA, IgM, C3, C4, focus score, 5-min salivary rate, ocular score, and Schirmer test (P > 0.05). Conclusions The memory B cell is highly expressed in pSS patients and their first-degree relatives and is negatively correlated with clinical indicators, suggesting that memory B cell may migrate from peripheral tissues to the gland tissues during the pathogenesis of pSS. BAFF level is remarkably higher in pSS patients than in their first-degree relatives and in healthy controls, suggesting that BAFF may be involved in the development of pSS but is not parallel with the severity of this disease. -
表 1 CD19 +CD27 +B细胞与临床资料的相关性
表 2 BAF F血浆水平与临床资料的相关性
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